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  1. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...breed. Some problems with transgenic mice that can impact reproduction include embryonic lethality, a short...should be tested in cell lines to determine how it impacts the expression level of the transgene. In existing...

  2. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...counterparts, so they will be easier to deliver in low-capacity vectors like AAV. AAV (adeno-associated viral ...tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into...

  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...that is complementary to the target site, or protospacer, in the genome. Native Cas9 has two catalytic...which cleaves one strand of DNA upon binding the protospacer. The resulting double strand break (DSB) stimulates...

  5. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... of the protein’s backbone in three-dimensional space, but their flexible and jiggly nature suggested ...was short-lived as powerful molecular graphics packages were soon developed that allowed researchers to...

  6. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...a team to develop therapies that could directly impact patients. And, to be honest, I’ve never enjoyed...better understanding of the fast, deadline-driven pace of biotech. In other words, industry postdocs already...

  7. PhD Applications After COVID

    Type
    Blog Post
    ...with picking universities?” and “How does COVID-19 impact all of this?” are all very valid questions, and...program will allow for you to have the time and space to delve into what matters to you. It can also be...

  9. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...genomic integration. Lab-engineered viruses will package your genetic cargo in lieu of their own genomes...genomes and deposit this cargo upon host infection. Packaging viruses also have various serotypes – the surface...

  10. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...mutations which do not interfere with ACE2 binding, but impact recognition by specific anti-SARS-CoV-2 antibodies...intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus lugdunensis that...

  11. Common Injection Routes in Mice

    Type
    Blog Post
    ...injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular...cerebral spinal fluid. The injection site is the space in and around the vertebrae, so hitting the correct...

  13. Antibody Guide

    Type
    Collection
    ...antibodies are collected, tested against the antigen, packaged, and sold. This is the most cost-efficient way...antibodies to upwards of twenty. Laser and software capacity theoretically allow for roughly fifty different...

  14. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...event-planning base and will know how to reserve space, time events, and network for volunteers. Speaking...curriculum, would be hard-pressed to maintain the pace of CRISPR. Two of the examples I used in my presentation...

  15. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...decided to take a different approach. His own most impactful learning experiences happened in the lab, and ...analyze their recently purified MDH to study the impact of phosphorylation on the enzyme’s structure, function...

  16. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...well-documented history of racial bias, which still impacts and influences current research. Understanding ... reflects European ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency...

  17. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...much the spread of the virus to adjacent areas impacted the experiments we wanted to perform. It required...injection titers (1,2), and cell types used for packaging viral particles (6), can influence vector performance...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...

  19. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...Cas9’s, but with the direct repeat preceding the spacer rather than following it. The Cpf1 crRNA is also...counterparts, they will also be easier to deliver in low-capacity vectors, such as adeno-associated viral (AAV) ...

  21. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...application and submitting it, to evaluating the capacity of an organization to receive and manage funds...up-to-date on the changing guidelines and regulations impacting grant management, we track grant opportunity deadlines...

  22. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...from quenching by aqueous solvent. This tightly-packed structure explains the importance of the entire...stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be used, when...

  25. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...colleague and give you a million dollar startup package. There are three steps to chalk talk preparation...have developed your research vision, you need to package it as a roughly 30 minute chalk talk. You will ...

  27. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...sharing knowledge allows for life to happen without impacting the overall work. Each of our sprints is themed...

  28. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... clearance. It is more convenient to update the spacer(20nt) on pEcgRNA plasmid as only two 24 nt oligos... proteins Cas6, Cas7, Cas8, and the crRNA. The spacers of the crRNA can be customized as well, including...

  29. How to Be an Excellent Trainee

    Type
    Blog Post
    ... walking through the protocol, by going to each space in order to double-check you remember where the ...Resources Resources on the Addgene blog Trading Spaces – Visiting Scientist Edition Mentoring for Scientists...

  30. Five Popular Model Organisms

    Type
    Blog Post
    ...mice to study how different mutations in leukemia impact different treatment regimens (Zuber et al., 2009... our own cells. Like human cells, yeast DNA is packaged into chromosomes and about 23% of yeast genes ...
Showing: 301 - 330 of 556 results