Addgene

We narrowed to 362 results for: Pol

Showing: 301 - 330 of 362 results

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New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Type

Blog Post

... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ...
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Type

Blog Post

...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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Blog Post

... Marc Sze, Victoria McGovern, and Julie Wolf. Apologies to anyone I’ve inadvertently left off this list...
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Type

Blog Post

...Wieschaus. "Mutations affecting segment number and polarity in Drosophila." Nature287.5785 (1980): 795-801...
Lab to Office Culture Shock

Type

Blog Post

...of the causes of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list...
Deciphering the Mysteries of Behavior with Viral Vectors

Type

Blog Post

... psychiatric diseases such as schizophrenia or bipolar disorder. In both diseases, animals have reduced...
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Type

Blog Post

..., including investigations into environmental pollutants and health, embryo growth, brain function, and...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...
CRISPR 101: Targeting RNA with Cas13a (C2c2)

Type

Blog Post

...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...
The Challenges of Cell Culture

Type

Blog Post

...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...
Plasmids 101: E. coli Strains for Protein Expression

Type

Blog Post

...induction system. The DE3 lysogen expresses T7 RNA polymerase (RNAP) from the bacterial genome under control...
Adeno-associated Viruses (AAVs) for Genome Editing

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Blog Post

...choice. These vectors are very similar, both with polylinkers for the addition of gene-specific 1 kb homology...
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Type

Blog Post

...mitochondrial matrix (Rhee et al., 2013) and determine the topology of the mitochondrial calcium uniporter (Martell...
Antibodies 101: Introduction to Immunofluorescence

Type

Blog Post

...common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

...Bendezú, Felipe O., et al. "Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based...
Harnessing Bacterial Toxins for Allelic Exchange

Type

Blog Post

...tuner: a computer program for the construction of polylinker sequences of molecular vectors". Proceedings ...
Multiple Plasmids at a Low Price: Kits from Addgene

Type

Blog Post

...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

... Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG If you need more...
CRISPR 101: Epigenetics and Editing the Epigenome

Type

Blog Post

...https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy...
Is this the right place for me? 8 tactics for choosing a lab

Type

Blog Post

...treat one another (especially those low on the totem pole). Can you see yourself enjoying this atmosphere?...
Finding Your Perfect Job After University

Type

Blog Post

...and non-profit) you can also get a lot of office politics (many individuals trying to divert more resources...
Sequencing Options for CRISPR Genotyping

Type

Blog Post

.... 2. Vouillot, Léna, Aurore Thélie, and Nicolas Pollet. "Comparison of T7E1 and surveyor mismatch cleavage...
Transferable Skills Guide: Teamwork

Type

Blog Post

...scientific team came as an undergrad at Worcester Polytechnic Institute. Though some WPI students chose to ...
A History of Genome Engineering in Popular Culture

Type

Blog Post

...what would happen if these corporations would extrapolate genetic engineering to exploit human beings. ...
A Guide to Getting Started in Undergrad Research

Type

Blog Post

...dedicated to different career options from science policy to science communication and much more. I hope ...
Mycoplasma Contamination: Where Does It Come From and How to Prevent It

Type

Blog Post

... Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction. In: Methods in Molecular Biology...
Prime Editing: Adding Precision and Flexibility to CRISPR Editing

Type

Blog Post

...transcriptase domain copies the desired edit, directly polymerizing new DNA onto the nicked target strand. The initial...

Showing: 301 - 330 of 362 results

Results per page:

New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Lab to Office Culture Shock

Deciphering the Mysteries of Behavior with Viral Vectors

Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Overcoming the AAV Size Limitation for CRISPR Delivery

CRISPR 101: Targeting RNA with Cas13a (C2c2)

The Challenges of Cell Culture

Plasmids 101: E. coli Strains for Protein Expression

Adeno-associated Viruses (AAVs) for Genome Editing

Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Antibodies 101: Introduction to Immunofluorescence

Antibodies 101: Single Chain Fragment Variables (scFvs)

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Harnessing Bacterial Toxins for Allelic Exchange

Multiple Plasmids at a Low Price: Kits from Addgene

Using Phosphoserine to Study Protein Phosphorylation

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Antibodies 101: Multiplex Immunofluorescence

CRISPR 101: Epigenetics and Editing the Epigenome

Is this the right place for me? 8 tactics for choosing a lab

Finding Your Perfect Job After University

Sequencing Options for CRISPR Genotyping

Transferable Skills Guide: Teamwork

A History of Genome Engineering in Popular Culture

A Guide to Getting Started in Undergrad Research

Mycoplasma Contamination: Where Does It Come From and How to Prevent It

Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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