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  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...We've discussed promoters before so in this blog post we’ll cover basics of multicistronic vectors: why... plasmids. We've combined our Plasmids 101 blog posts and additional resources into one downloadable PDF... 2008 May 11.) PubMed.   Read more Plasmids 101 posts or browse Addgene's Plasmid Guide for more molecular...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...are transcribed from the RNAP III promoters. This post features promoters for general RNAP II and RNAP ...constructs and we will discuss these in a future post on viral vector parts. Promoter specificity Aside...Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter...

  3. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...This post was contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at...continue my scientific endeavors. I hope that this post can provide some perspective and advice to any undergraduate... you. Be proactive Rather than applying only to posted job offerings, the best research experiences typically... offer application fee waivers. One of the blog posts that helped me quite early on was Christine Liu’...graduate and/or undergraduate classes Postdoctoral researcher (postdoc) Getting more scientific and professional...or 9-to-5 job. I had to ask to find out what a “postdoc” is, and that “PI” stood for principal investigator...admin duties Staff scientist: similar role to a postdoc but more permanent Visiting scholar/student: visiting...

  4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ... sound almost too good to be true! In a previous post, we covered systemic capsids, which allow AAVs to...tissues like the central nervous system. In this post, we’ll dive into the opposite: AAV serotypes with...ll cover the most commonly used serotypes in this post. However, tissue tropism varies both between species...AAV2-retro) We’ve covered AAVrg in a previous blog post, but to summarize briefly: AAVrg is derived from...

  5. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...This post was contributed by Kartik Lakshmi Rallapalli, a graduate student at the University of California...preference than the ZFNs.   Genetic Engineering Era: Post CRISPR  CRISPR The strategy of using a protein to...the Genome Editing Trading Card seen in this blog post?Download high resolution versions here.  Many thanks...Additional resources on the Addgene blog Find more blog posts about CRISPR Learn more about the history of CRISPR...

  6. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated with a ...

  8. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...which contains the viral genome. In today’s blog post, we’ll focus on the AAV transfer plasmid and take...and combinations out there not discussed in this post, you’ve got the knowledge to be able to design a...WPRE The Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary...

  9. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...for our first post, I thought it best to define language you’ll see throughout the posts and introduce...field, often through manuscripts, seminars, or posters. It is important to note that it is the actual ...

  11. The Strength of Story Telling

    Type
    Blog Post
    ... This post was contributed by guest blogger Jack Leeming Editor of Naturejobs, part of Nature magazine...Resources on the Addgene Blog Check out our blog posts on science communication Find interviews scientists...

  12. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...graduate student in the Roth lab, for editing the blog post. References Olsen RHJ, DiBerto JF, English JG, Glaudin...blog Browse all of our fluorescent protein blog posts Find other fluorescent biosensors Resources on ...

  13. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...) and subject age at time of injection. How long post-injection did you wait until performing the experiment...process of designing your experiment, read our blog post on important considerations when using AAVs. Experimental...

  14. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...For more details on this technology, see our blog post Overview of AAV Encoded Calcium Sensors). Plasmid...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral ...

  16. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...This post was contributed by guest blogger Sean Stacey who recently attended both online and on site ...publications. My classmates consisted of graduate students, post-grads, and early career scientists. Over the course...

  17. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...vector educational resources as well, with both posts on the blog and pages on the Addgene website. blog.addgene.org...virus. You can find all of our viral vector blog posts here. If you have virus expertise you’d like to ...

  18. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...This post was contributed by guest blogger Meghan Monroy, a graduate student in Protein Science at the...on Your Plasmid Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for...

  19. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional Resources on Addgene.org Check Out ...

  20. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...communication skills. So, where can a graduate student or post-doc start? The good news is there are more opportunities...science on the one you already have. Read my earlier post about Scientists on Twitter here. 4)   If you enjoy...

  21. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... Plasmids 101 blog posts Learn more about the origin of replication Find blog posts about plasmid cloning...maintained in one bacterial cell. In this Plasmids 101 post, we’ll cover why this happens, how it might affect...

  22. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...Professor at Georgetown University, contributed to this post. Adeno-associated viruses (AAVs) make fantastic...enhance the efficiency of genome editing. In this post we'll walk through a variety of ways you can use...many applications of AAV in some of our other blog posts and have also been able to browse some of the ready... al. "CRISPR/Cas9-mediated genome editing via postnatal administration of AAV vector cures haemophilia...

  23. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...chemotherapeutics, and ultraviolet light. In this post, we will describe the mechanism of homology directed... than 10 bp away if possible. Check out our blog post on Improving HDR efficiency for more details on ...highest frequency of HDR edits.  Check out our blog post on Easi-CRISPR for more details. References and...single-stranded DNA templates. Jacobi SA, et al. Poster at Keystone Precision Genome Engineering. 2017....

  24. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...This post was originally written by Joel McDade and significantly updated in 2022 by Susanna Stroik.  ...verify your genome edit can be found in our blog post entitled “CRISPR 101: Validating Your Genome Edit...More resources on the Addgene blog Read all the posts in our CRISPR 101 Series Learn about the PAM Requirements...

  25. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...This post was contributed by guest blogger Derek Simon. The actual experiments you do will be determined...topic you’re interested in studying, but, in today’s post, we’ll discuss some of the important considerations...optogenetics to be fully summarized in a short blog post, but some examples I’m personally interested in ...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

  26. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...This post was contributed by Damien Wilpitz founder and lead consutant at Experiment Designs Consulting...start up budget, create a start up list.  In this post we’ll help you successfully negotiate a start up...        Additional Resources Find other career posts on the Addgene Blog! Learn more about Experimental...

  28. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...This post was contributed by guest blogger Talley Lambert, a Research Associate at Harvard Medical School...online guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining..., using a popular database management system (PostgreSQL), that aims to eventually be a comprehensive ...

  29. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...For more details on this technology, see our blog post Overview of AAV Encoded Calcium Sensors). We are...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral ...

  30. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ... This post was contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. ...Additional resources on the Addgene blog Find blog posts on scientific sharing Get tips on writing a review... submission to the subsequent journal.  As a postdoctoral researcher studying plant development and abiotic...HapMap population in Amsterdam. Currently, she is a postdoc at KAUST, Saudi Arabia, in the group of Prof. Mark...
Showing: 301 - 330 of 782 results