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We narrowed to 569 results for: CIT

Showing: 201 - 220 of 569 results

  1. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse...Transcriptional Devices Dependent On GFP). In testing the specificity of their system, Tang et al. found that YFP and... using the rTetR system, but Cre-DOG lacks the toxicity that can be seen with high levels of transcription...

  2. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy...

  3. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...on the position in the protospacer, the editing capacity of the ABE8s ranged from 1.5x higher for positions...For applications that require high DNA editing specificity, for example for potential therapeutic approaches...ABE8s demonstrate an overall improved base editing capacity, even at sites that used to be difficult to target...

  4. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...antibody’s specificity. Strength and accuracy The strength is based on binding specificity and exposure...public service to the science community, and I am excited to be a part of it! If you are interested to get...

  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...rate (Redfern et al., 1999) or altered neuron excitability (Zhu et al., 2014). The system allows you to...important to consider that the same receptor can elicit different responses depending on what cell type... chemogenetic receptors can be used to alter excitability by lowering or raising the threshold for signaling...

  7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...trials, wild type AAV is not associated with any pathogenicity and has a low immunogenic profile. AAV was first...new and refined forms of capsids with improved specificity. AAV vectors are able to facilitate and enhance...abilities to infect target tissues. Improved specificity through control of gene expression and the selection...

  8. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ... the Science City The conference was located in the modern, relatively new “Science City” area on the...choose to locate there. I made time to go into the city of Guangzhou and see some of the sites. Most fascinating...

  9. Of Myc and Men

    Type
    Blog Post
    ...this, there are close to 10,000 citations for anti-Myc 9E10 in CiteAb, many of which are from this year...Locate a promising monoclonal antibody with high specificity. (2) Narrow down the specific epitope that your...

  10. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...moments. The models function as thinking tools – eliciting questions like “what does this model representt...proteins for researchers. Modeling CRISPR We were excited when Jacob Corn contacted us in 2014 to ask if ...in a rationally engineered Cas9 with improved specificity. Hold this flexible model in your hand for a ...

  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...light by using a pinhole.  However, the excitation light excites all of the fluorophores as it passes through...order to improve axial resolution and reduce phototoxicity for imaging live specimens, they combined light...

  12. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...history was very much in the making. One of the exciting, and challenging, aspects of talking to 8th graders... proficient in science, regardless of race or ethnicity. Perhaps you don’t have children but are interested...strawberry, or demonstrated that they’re living in an exciting, tumultuous time in scientific history. Whatever...

  13. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...application and submitting it, to evaluating the capacity of an organization to receive and manage funds... was moved from one department to another within city government. Throughout these moves and program changes... our program didn’t stay in one place within the city infrastructure. My career path changed when I was...

  14. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... however, either lack the sensitivity or the specificity to detect nucleic acids at low concentrations...atomolar sensitivity and single base pair mismatch specificity.  SHERLOCKv2 SHERLOCK had a few limitations when...detect nucleic acids with high sensitivity and specificity without compromising speed, ease of use, and ...

  15. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...community is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate ...programs for K-12 students. You can see why I was excited to be invited to attend. My job is to ensure that...marmorated stink bugs to make them pink. We’re excited to see new tools being developed and shared for...

  16. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...single molecules of an RNA species with high specificity (Gootenberg et al., 2017). This system, dubbed...Cas13a in mammalian cells The Zhang lab made the exciting discovery that some Cas13a orthologs can function...Cas13b as a tool for RNA base editing,, and we are excited to see this group of enzymes continue to make a...

  17. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...or “Do you have additional data regarding the specificity of this antibody?” You will also help troubleshoot... customers everyday, it can be fascinating and exciting to listen to the many individual stories and get... of science communication, and is particularly excited to hear about novel developments in the fields ...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...plasmids, optimized for a variety of species.   Specificity concerns? If off-target effects are a worry, ...nickases (Cas9n) to the target locus, enhancing specificity over a single SpCas9. The drawback of this approach...and consider using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9...

  19. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...something that could move your project onto the exciting—and unanticipated—road of discovery. Monitoring...inhibition), and behavioral (remote memory retrieval) specificity. Optogenetics can be used to more precisely associate....    References 1. Stuber, Garret D., et al. "Excitatory transmission from the amygdala to nucleus accumbens...

  20. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ... appropriate as they allow you to assess the specificity of your observed result. Non-targeting controls...important reference point when analyzing the specificity of the shRNA targeting human Gene X. The non-...describe these a bit more below, but the papers cited in the reference section provide more in depth information...
Showing: 201 - 220 of 569 results