We narrowed to 1,027 results for: ELL

...receptors to selectively control and investigate cell signaling pathways. Receptors are remotely controlled...and genetic engineering to measure and manipulate cells (frequently neurons) and their governing biomolecular...detect, measure, and control molecular signals and cells in order to understand their functions. Read More...

...element controls the rate of transcription. Bacterial cells contain sigma factors that assist the RNA polymerase...other promoters can drive expression in specific cells or under different conditions. Promoter Expression...ribosomal RNA (rRNA), which is a main component of a cell’s ribosome structure. Ribosomes are the site of protein...

...another. The size of the primer is very important as well. Short primers are mainly used for amplifying a ...

...clothing from potential spills. Gloves should fit well enough to be snug to your hand, but not so tight...

... a wider gel comb; or c) loading less DNA in the well. How do you get better separation of bands? If you...

...Sequence Scanner (PC), and Chromas (PC). How can I tell if a peak is mis-called? Open the trace file and...

...restriction digests needed in molecular biology, and thaw cell lines. There are many different types of water baths...

...the correct orientation. For detailed protocols as well as tips and tricks see the Restriction Digest and...

...same amount of liquid into different tubes or into wells of a microplate, you may consider using a multichannel...

...Note: (Link opens in a new window) Primer3 is an excellent resource for choosing primers. Place thin-walled...

... the plasmids of interest (for example, fewer satellite colonies will grow). However, it is also more ...

...homology. While this approach doesn't work in other cell types, it can be a major time- and cost-saver for...

...plot of CD3 (T cell marker) versus CD19 (B cell marker) to separate the B cell and T cell into distinct...separate the B cell population into progenitor B cells, immature B cells, plasma cells or others.   ...determining cell health, analyzing cell cycle stages, and detecting specific populations of cells in a complex... the cellular level, it cannot be used for subcellular analysis such as morphology or subcellular localization.... As cells pass through the flow cell the size of the sample stream is reduced forcing the cells to line... photons varies from cell to cell depending on the level of fluorescence. A cell expressing high levels...compatible with 96-well plates as collection vessels and can sort single cells into each well allowing users...

...out all CD8+ T cells from a mix of CD8+ T cells, CD4+ T cells, B cells, and dendritic cells. In this case...dumped”. From left to right: B cell, CD4+ T cell, dendritic cell, CD8+ T cell. Created using biorender.com...in flow cytometry are cell proliferation and cell viability. Unlike intracellular labeling, dye staining...be done on live cells. For cellular proliferation, esterified dyes that can enter cells freely are typically...amines on the cell surface, which yields a much weaker signal on live cells compared to dead cells. As a result...comes to labeling cells for flow cytometric analysis, the most common method is a cell surface label, where...through the cell membrane freely, while others require a permeabilized membrane to enter cells. Depending...

...activity, and consists of other cell types, especially immune cells and cells of epithelial origin. Suppressing...turns off T cell function upon interaction with APCs. Furthermore, tumor cells alter their cell-surface proteome...molecules needed for T cell effector function. Cancer cells also produce programmed cell death ligand (PD-L1... activity of CTLs and NK cells. These cells would normally kill cancer cells but are directed not to through...to use whole cell therapeutics consisting of various types of immune cells. Dendritic cells (DCs) are particularly... intravenously. B cells and T cells activated ex vivo are also contestants for cell-based immunotherapy... include: Cell heterogeneity Tumors are composed of a wide variety of cell types. This cell heterogeneity...

...Many cell types can be found in the tumor microenvironment (tumor cells, immune cells, blood cells and ...about 53% of my cells are dendritic cells and ~13% are CD8+ T cells. Around 34% of the cells are neither ...neither of the two cell types (likely B cells and others). Almost no cells stain positively for both markers...measure your cells of interest. In many experiments you’ll be working with a heterogeneous cell population...processed piece of tissue, where cells are present that vary in cell type, size, and marker proteins. ...analyzing a homogenous cell population in culture, it is normal to observe diversity in cell size, marker expression...illuminates the passing cell with a laser and detects the light scattered by that cell at a low angle (FSC...

...Pluripotent Stem Cells by Direct Delivery of Reprogramming Proteins." Cell Stem Cell 4, no. 6 (2009): ...Jaenisch. "Induced Pluripotent Stem Cells Meet Genome Editing." Cell Stem Cell 18, no. 5 (2016): 573-86. PubMed...Reprogram Peripheral Blood Cells Check Out Our Interview with Stem Cell Researcher Darrell Kotton Four Factors... express a cocktail of stem cell reprogramming factors and wait for cells to de-differentiate. However...transgene-free and vector-free cells Genome integration Need to sequence cells to verify excision didn’t ...vector-free cells No genomic integration Can be difficult to fully remove virus from cells Plasmid Non-integrating...transduces a wide range of cell types and replicates in the cytoplasm independent of the cell cycle. A challenge...

...label an intracellular target.   Figure 1: HCT 116 WT and SYT1 KO cells were labelled with a green...levels in your cell lines, you can consider a cell treatment approach. If there is a cell treatment (e....antibody shown to perform well in one set of conditions may not perform as well in other conditions. For...select a candidate cell line (e.g. DepMap) – you’ll need to ensure that the parental cell lines of your chosen...the parental cell line, but not in the iso-genic knockout control. This can involve cell surface labeling...selectivity for cell surface labeling may become less selective if used in intracellular labeling because...because the intracellular compartment may contain cross-reacting antigens not present on the cell surface....

...performed on whole-cell extracts from the initial stable cell pool (pool) and from individual cell expansions ... in several human cancers) in A549 cells (a human lung cancer cell line), armed only with viruses obtained...need our A549 cells to express Cas9. In these particular experiments, we generated A549 cells stably expressing...52962-LV, Figure 1). Some cells were then used to generate a monoclonal cell lines by limiting dilution...The rest of the cells were frozen down. Figure 1: Cas9 expression in A549 cells transduced with...blotting was performed on whole-cell extracts from stable pools of A549 cells transduced with Cas9 expressing...A549 cells are relatively easy to transduce. I ended up with too many Cas9 integrations per cell, and ...

...experiments including subcellular visualization of 6 different organelles, a cell cycle indicator for mouse...recorded in the cell's DNA under the right conditions, and could initiate specified cellular processes or...Understanding intracellular pH regulation is important as pH regulation has roles in many cellular functions...signaling pathways to the cell cycle. Good tools for measuring intracellular pH are required in order ...useful tools for light-mediated subcellular localization in mammalian cell culture and reversible control...in live cells is that one can now follow movement of two or more targeted loci during the cell cycle or...mammalian cells, yeast, and Drosophila. Synchronization of secretory protein traffic in mammalian cells The...