We narrowed to 464 results for: LARGE

1. Plasmids 101: TOPO Cloning

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   Blog Post

   ...low concentration or you are cloning an extremely large insert. Since the standard ligation reaction is...

2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   Blog Post

   ...rates of approximately 1%. This is by no means a large number, but it was attained without any of the error-prone...

3. Resume Writing for Non-academic Science Careers

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   Blog Post

   ... data, then the chance is that you have handled large data sets and are familiar with statistical analysis...

4. History of CRISPR Cas - A tale of survival and evolution

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   Blog Post

   ...translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR systems between and ...

5. Brain Initiative Collection

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   Collection

   ...development and application of innovative tools enabling large-scale real-time interrogation and monitoring of ...

6. CRISPR Plasmids - Empty gRNA Vectors

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   Collection

   ...S. pyogenes BFP Kuhn pLKO.1-puro U6 sgRNA BfuAI large stuffer 52628 Mammalian/Lentiviral BfuAI none S....

7. Learning Technical Skills at a Lab Central Workshop

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   Blog Post

   ...central We’re working on a project where we aim to do large scale DNA preps with multiple samples without cross...

8. Plasmids 101: Inducible Promoters

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   Blog Post

   ...therefore determines the timing of gene expression and largely defines the amount of recombinant protein that ...

9. A Novice's Perspective on Neuroscience - SfN2017

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   Blog Post

   ...neurobiology (and really in all of biology) require large data sets. Whether these data sets include videos...

10. A Deep Dive into BioBuilder

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    Blog Post

    ...for all who wish to participate. All new teams, large or small, receive the BioBuilder textbook, a laboratory...

11. Antibodies 101: Monoclonal Antibodies

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    Blog Post

    ... the antibody, meaning that the antibodies are largely identical between lots.  Disadvantages of monoclonal...

12. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post

    ...to generate these antibodies were based off of a large collection of mouse monoclonal antibodies (mAbs)...

13. Sweating the Small Stuff: Details in the Lab

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    Blog Post

    ...  Prevent Interruptions If you need to focus, a large set of headphones can act as a Do Not Disturb sign...

14. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    Blog Post

    ...with an average of 206 cells per TF ORF. From this large sequencing endeavor, a map of possible TF-induced...

15. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    Blog Post

    ...instrumental in interpreting human variation at large scales and making personalized medicine a reality...

16. Targeted Mutagenesis with EvolvR

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    Blog Post

    ...phenotypes, and forward genetics harnesses this at a large scale by screening diverse libraries of genetic ...increasing the editing window of EvolvR to ~56 bp. A larger editing window also increased the likelihood that...

17. Four Factors that Differentiate the Stem Cell Field

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    Blog Post

    ...affected the stem cell community – a community largely created due to Yamanaka’s seminal work. It didn't...

18. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post

    ...making science easier and more transparent. That’s largely my job [at the Broad] too… to make science easier...

19. 3 Challenges in Plant Synthetic Biology

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    Blog Post

    ...pieces developed over the years were generated by large agricultural companies and carry associated intellectual...

20. Plasmids 101: Mammalian Vectors

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    Blog Post

    ...virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (293E or 293T cells), allow for episomal...