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We narrowed to 741 results for: REV

Showing: 201 - 220 of 741 results

  1. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...Mechanisms. Annual Review of Biophysics, 46, 505–529. https://doi.org/10.1146/annurev-biophys-062215-010822...lobes are inactive in the absence of guide RNA, preventing Cas9 from making off-target cuts.   Pro tip! ...CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/...High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nature...

  2. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...brain-visceral organ signaling. The problem  Previously, there were very few tools available for monitoring...   To develop these fibers, the labs looked at previous implementation of optogenetic tools, calcium sensors...stimulation using NeuroStack and the fibers as previously described, then measured electrophysiological...optoelectronics for neural interfaces. Chemical Society Reviews, 48(6), 1826–1852. https://doi.org/10.1039/C8CS00710A...

  3. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...real, peer-reviewed study. The second group read the same abstract but with the names reversed, meaning ...looked at how people perceive and evaluate peer-reviewed sources of information if those sources affirm...meaning that the findings of the study were also reversed. The third group read a version of the abstract where...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...of the fluorescent protein dsRed that changes irreversibly its color from green to red when it is oxidized...et al., PLOS One 2013 Oct 7;8(10):e76117. Irreversible peptide-peptide ligation using SpyLigase Building...excitation of neurons. The paper describes five previously unknown channelrhodopsins from different species...designed two vectors for use in conditional and reversible gene silencing in RNAi transgenic mouse models...Jesse Rinehart and Dieter Söll at Yale University previously changed the way researchers can explore important...engineering demonstrate improved efficiency over previous reports. For additional information on using ...

  5. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...identified have little sequence similarity to previously identified Cas13a-c orthologs, but they do include...system. Since Cas13d is functionally similar to previously discovered Cas13 enzymes - what makes these orthologs...80% for other Cas13a and Cas13b effectors. Like previously characterized Cas13 enzymes, CasRx also displays... manipulate RNA species through exon skipping. Previous CRISPR exon-skipping approaches used two guide...

  6. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...and exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient signal for in vivo...increased sensitivity and faster imaging compared to previous publications. They also developed calcium, cAMP...described in Suzuki et al. improve the brightness of previous Nano-lanterns by 2-4 fold and permit five-color... its substrate furimazine. In addition to the previously described applications, the enhanced signal of...

  7. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ... pR, preventing expression of a reporter gene. When light is present, YFI is inactive, preventing repressor...inactive because a bound repressor protein actively prevents transcription. Once an inducer binds the repressor...activation. In this system, the activator rtTA (reverse tetracycline-controlled transactivator) is normally...

  8. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...reporters in the near future.  SRIRACCHA (stable, but reversible, integrated reporter for assaying CRISPR/Cas-...target region into the N-terminus of mCherry. To prevent alternative start site initiation, the lab removed... this one mutated methionine was not enough to prevent alternative initiation, so they mutated the methionines..., Davidson NO, Madison BB (2017) A stable but reversible integrated surrogate reporter for assaying CRISPR...

  9. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...lot of trial and error.” The structural details revealed two exposed RNA loops, which Konermann recognized...of all, the Cas9s can be turned on and off, to reversibly activate or repress genes of interest. They also...in your experiments, Addgene recommends that you review the steps for how to use pooled lentiviral CRISPR... CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  10. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...treatment regimen and were not preexisting. This was previously debated in the cancer field and was difficult...be amplified using the same sets of forward and reverse primers). The abundance of a particular barcode...replicate; prior to treatment, there are small (previously undetectable) populations of cancer cells that...that combination treatment with multiple drugs reveals even smaller preexisting populations with resistance...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...In previous posts for our Plasmids 101 series, we examined a number of important plasmid elements – promoters...and the region of DNA between the loxP sites is reversed.   Deletion: If the sites face in the same direction... cassette) upstream of a gene of interest will prevent gene expression in the absence of Cre. In the presence...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-...

  12. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo et al. Nat Commun. 2019...options. While the BiFC interaction is typically irreversible and requires some time for chromophore maturation...maturation, splitFAST displays rapid and reversible complementation. TagBiFC (split HaloTag) provides maximum...important for their interaction. You can read our previous blog post for more on BiFC principles and experimental...

  13. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To see even more ...uses loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a colorimetric readout...completed in just 45 minutes. The team deposited the reverse transcriptase, protease, and Bst polymerase constructs... this toolkit include ones for expressing MMLV reverse transcriptase and Taq polymerase that can be used...

  14. 6 Tips for Grant Writing

    Type
    Blog Post
    ..., you will need to correct it). It’s easy for reviewers to spot when an application is rushed (whether...that you ask a PI or mentor (your supervisor/ a previous supervisor / someone else in the department) to... times and will have useful advice about what reviewers in your area are looking for. Remember though,...for grant success The two most important things reviewers will be looking for in your research proposal ...

  15. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...broken during cross-link reversal (not shown in diagram). Cross-links are reversed so that DNA fragments ...cells (~5x107). Together, these two requirements prevent the use of CAPTURE with primary cells or rare cell...multiplexed CAPTURE 2.0 largely replicated interactions previously identified with the original CAPTURE method. ...

  16. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ... are voltmeters for cells! In this blog we will review how these indicators work, their general applications...of the membrane, a concern with several of the previously described systems. Several adaptations of this...   Viral Vectors and Voltage Indicators As previously mentioned, cellular voltage is measured at the... Components of the types of voltage indicators reviewed.   References and Resources   References ...

  17. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...new managers and I had a “Eureka” moment while reviewing the results of my Myers-Briggs test:  my undergrads...motivation, but he didn’t want to spend hours reviewing papers to piece together the best protocol;  he...associate feedback with end-of-the-year performance review, but feedback should be given throughout the year...References Becoming the boss. Harvard Business Review, January 2007. Three Rules for Managing a Successful...

  18. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...then dropped to a range in which the forward and reverse primers are stable. At this temperature the primers...can also be used to quantitate RNA levels using reverse transcription-qPCR (RT-qPCR). The first step of...process requires RNA to be converted to cDNA using reverse transcription and the cDNA is subsequently quantified...template is amplified using several forward and reverse primer sets.  Multiple template PCR - multiple...

  19. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...Cas13-based RNA editing system would likely be reversible and would avoid genomic off-targets or indels... RNA knockdown. In initial tests, Cas13b from Prevotella sep. P5-125 (PspCas13b) yielded 62.9% average...editing plasmids here! Future directions Building on previous RNA targeting work with Cas13, REPAIR is the first...edit RNA has multiple advantages, including reversibility and use in non-dividing cells. With the expanded...

  20. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...ambitious, that’s because it is. Zimmer, whose previous books focused primarily on fluorescent proteins...racism and sexism in science, problems with peer-reviewed publications and funding, bioethics in the age...unlikely to be surprised by many of Zimmer’s revelations. The issues he discusses have been making the...of his book today--the day you are reading this review--in the current period of rapid flux, the chapter...
Showing: 201 - 220 of 741 results