...interview is another way to make the virtual environment more comfortable and help you to keep yourself...institution, you can get a better sense of the environment of the department, the lab setting, and how students... can also be nerve wracking to ask questions in front of other prospective students; an online meeting...by talking to current students about the work environment and social aspects of the department, you may...Options Thanks to the much more developed virtual environment resulting from the pandemic, students who are...

...images. In the early days of structural biology, Byron Rubin, a crystallographer working with Jane Richardson...solution to this problem with the development of Byron’s Bender – a mechanical device that could be used...protein’s structure into experimentally determined electron density, have been known to say something silly... in the education community that All models are wrong….Some models are useful. While a physical model ...first Cas9 model is certainly useful, it is also wrong in at least one serious way. We represented the ...

... having already been immersed in an industry environment, have a better understanding of the fast, deadline-driven...up in an industry job, being immersed in that environment sooner rather than later can be helpful. Publishing...project might be challenging. In an academic environment, surrounded by lab members who are all working...Novartis, Biogen, Amgen, New England Biolabs, Regeneron, and Genentech, among others, and the list is ...opportunity to do some great science in a supportive environment. Both paths will give you myriad opportunities...

... In LSFM the laser light sheet, typically 2-6  microns, illuminates only one thin plane of the sample ...image acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...of transgenic mice in which a subpopulation of neurons are brightly labeled with GFP (e.g. Thy1-YFP-H ...LSFM with multiple tissue clearing strategies and neuronal labeling methods will greatly aid our understanding...331–336. Pubmed. P. Keller, M.B. Ahrens.  (2015) Neuron, 85, 462-483. Pubmed. E.H.K. Stelzer. (2015). Nature...

...dimeric green fluorescent protein (pdDronpa) (Zhou et al., 2018). pdDronpa dimers dissociate in response to... to cyan light (500nm). By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft...binding in the dark. Upon light stimulation, the pdDronpa moieties move apart, allowing DNA binding and ...Cas9, dCas9 Gene editing Zhou et al., 2018   pdDronpa Cas9, dCas9 Gene editing, transcription Light-Dependent...photoactivatable transcription systems to induce neuronal differentiation. Nat Methods 14:963–966 . https...

...cloning capacity of ~8 kb, so either a single polycistronic vector or multiple single-gene vectors can be...endogenous pluripotency genes. Retroviral vectors are prone to incomplete silencing of reprogramming transgenes...differentiation potential. Finally, retroviruses are prone to insertional mutagenesis and random integration...retroviral vectors. This makes it possible to use a polycistronic cassette that expresses multiple reprogramming...factors can be delivered either as a single polycistronic transgene or with four different adenoviruses... ~14.5kb minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG...

...dependent on the photon budget and pushing the frontiers of fluorescence microscopy often requires more...easier and more functional in complex biological environments such as live cells and tissues (Figure 1). These...several others can be made fluorogenic using environmentally sensitive fluorophores. Overall, self-labeling...photon budget and allow us to push further the frontiers of biological imaging.  *Worried about your budget...Central PMCID: PMC4344395. 3. Lavis, Luke D., and Ronald T. Raines. "Bright ideas for chemical biology."...

...system that could do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus HIV-1...experimental use. In 1996, Naldini et al. transduced rat neurons in vivo with beta-gal and other reporters, and ...vectors are incredibly popular in the lab - the Trono lab’s lentiviral packaging and envelope plasmids...Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. Science. 1996 Apr 12;272(5259):263-7. PubMed ...Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. ...

...myself in a technical support role only when I was confronted one day with a product issue in the lab. The ...the world easier. You should also demonstrate a strong willingness to help others with their problems,...sometimes comprised of several layers of support: A front-line team accepts any incoming requests and answers...because there are just so many things which can go wrong in a lengthy protocol. And customers may tell all...careers are open to experienced professionals with strong interest in the technical aspects of science and...

...virtue of liberating data from paper into an electronic environment and hence making it searchable and shareable...founder of Research Space. There are many types of electronic lab notebooks (ELNs), each with its own pros ... of Research Space, which provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts...on the Addgene blog Find tips on choosing an electronic lab notebook Watch a livestream of the Minisymposium...

...CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson & Bird, 2019) and is a global...of symptoms in animal models, Rett syndrome is a strong translational candidate for gene replacement therapy...therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian Kaspar... half of the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has...Modifications in Human-Induced Pluripotent Stem Cells. Front Genet 10: . https://doi.org/10.3389/fgene.2019.00625...

...aspects of research at National Aeronautics and Space Agency, the Environmental Protection Agency, and the ...Additionally, interest groups are often pandered to when strong or overlooked when small, but data is not going...Science face deep cuts in Trump’s first budget Environmental Science and Technology: Crossing The Imaginary...having evidence’ on Trump’s wiretap claim The Chronicle of Higher Education: “March for Science” Organizer...

... and the linkage of intra- and extracellular environments. Found on nearly every cell in the human body...membrane, physically connecting the extracellular environment and the cytoskeleton. Most integrins have three...responses to mechanical forces in the cell’s microenvironment. The actin cytoskeleton exerts tensile force... by the ligand embedded in the extracellular environment. This further stabilizes the extended open integrin...integrin inhibitors and natural ligands, such as fibronectin, to block receptor function, e.g., cell adhesion...

...reviewer’s concerns, you are making your hypothesis stronger. When you share data at a conference poster session.... It can be extremely toxic in a lab or work environment if left unchecked. Substantive conflict occurs...handy: Empathy: If you often find yourself in confrontational situations with others because you simply cannot...Empathizing with people, especially people with whom you strongly disagree, is a critical component of conflict ...will be considered in the right, and one in the wrong. If you arbitrate, one party might get exactly what...

... increase diversity in the academic research environment. The “how many?” question lies at the base of... fact that training in the academic research environment is setup to prepare trainees for further work...receive as postdocs is not transferrable to other environments. Slide from Paula Stephan’s Talk (All slides...succeed in publishing in the current academic environment. Some great tips from this panel included:  ... the structural problems within the research environment itself. At Addgene we hope that all parties involved...

... the midst of a rapidly changing, globalized environment re-emerging from international financial struggle...but also nurture a supportive and protective environment where individuals are empowered to productively...opportunities that truly exist outside their immediate frontiers. Moreover, sharing a culture/country of origin...work? IMP Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant expertise and...and entrepreneurial visions thereby opening new frontiers for young talent around the world. The wealth ...

...climate change to study plant and marine biology, environmental microbiology, or biofuel production. There’s...Research requires dedication and effort, so building a strong understanding of what you’re getting yourself into...all just people. I’ve found that in the right environment and with supportive mentors, being open and vulnerable... what I don’t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what...the vital things to look for is a supportive environment—it makes all the difference in a lab experience...

...now available in Addgene’s repository for use in neurons, both in culture and in living brain tissue. “The...explained. “It helps us adapt to influences in our environments. We are able to react and learn. All of this... the LITEs to control gene expression in mouse neurons and in the brains of living mice both quickly and... want to apply LITEs for the transformation of neurons, she offers a tip: save yourself some time by making...

...fluorescent tags in neurons. Their toolkit named ORANGE (Open Resource for the Application of Neuronal Genome Editing...CRISPR/Cas9-based toolkit for epitope tagging in neurons Harold MacGillavry’s lab developed a CRISPR-based...targeted genomic integration of fluorescent tags in neurons.  Read the paper in bioRxiv Find the ORANGE toolkit...

...notebooks and binder on your desk. Arrange them in chronological order (or by project, if that works better for...can easily spot the colors and text. Tip 2: Use electronic to-do lists to access your tasks on the go Never...could easily lend one to another labmate? Though electronic lab notebooks (ELNs) have gained traction with...to borrow most frequently. Copy these into an electronic file sharing tool, such as Google Drive or Dropbox...