...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency...the reverse transcriptase. Many PE enzymes and related tools are available: PE2 - introduction of five...

...Thursday, September 20 6:00 PM-8:00 PM Group Dinner Relax and Connect with Fellow Speakers and Attendees Friday...you will have final approval before anything is released publicly, but please only share materials that...

...101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related protein LC3 B Autophagosomes 101784 SLC25A17-mEGFP...junctions 107579 RAB5A-mEGFP AICSDP-39 mEGFP Ras-related protein Rab-5A Endosomes 107580 AAVS1-mTagRFPT-...

...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (..., computational and experimental resources that relate to the control of gene expression in the grasses...

...lineage tracing in multicellular systems typically relies on genetic markers, somatic mutations, or labeling... previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled...

...our in-stock viral prep service.  AAV9 AAV9 has relatively broad tissue tropism and high transduction efficiency...

.... Speed: AAV transduction experiments are relatively quick. We have found that expression occurs quickly...roles of certain cell types in locomotion. We have relied heavily upon AAV vectors to deliver chemogenetic...AAV selection, there are three important factors related to location. Location (1): What tissue? Location... (2007): 1740-1743. PubMed PMID: 17882145. 2. Zincarelli, Carmela, et al. "Analysis of AAV serotypes 1...

...course, many types of teams. Some teams may be relatively permanent; some teams may only need to exist ...

...neurodegenerative disease because they had close relatives suffering from similar diseases. Others were inspired...t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what I thought was...

... day or a week planning an experiment, even a relatively simple experiment. However, by taking this time...fellowship grants) A project with sufficient preliminary data to establish your own lab and get an independent...Also, it is unlikely that a mentor has the only relevant expertise and perspective for your project. Furthermore...surprising findings when studying a seemingly unrelated problem. However, you have finite time and resources...Present your work often Surprisingly, many PFs are reluctant to give talks or posters about their research....requires evaluation of all of the data and any recent relevant studies in the literature. Plus, presentations...too much practice writing Young group leaders are rarely great writers. There are two ingredients to becoming...

... NOT Cre No Dual recombinase-dependent: Rabies-related Addgene ID Plasmid Logic Sites and Mutations In-Stock... of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs...

...years later, PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve...developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors directly...

...between 1,000 nm and 1,200 nm, where there is relatively little tissue absorption, weak tissue scattering...understanding of pathogen-immune cell interaction relied on in vitro studies where it was difficult to apprehend...experiments, experience has shown that some are more reliable and more efficient at tracking cells. Good two-photon...

...extract light from a single, thin plane within a relatively thicker three dimensional sample (see Figure ...great image is contrast. The human visual system relies primarily on contrast to identify individual objects...single camera exposure. This is much faster than relying on raster scanning as is done in confocal or 2-... possible. Therefore, 2-photon microscopy which relies on deeply penetrating infra-red excitation light...scanning confocal and 2-photon microscopes which rely on building an entire image pixel by pixel are unable...

...technique is remarkably straightforward to learn and relatively cheap (compared to many other technologies and...region can result in a particular neurotransmitter release or post-synaptic current. Fortunately, if your ...just optogenetics. Successful stereotaxic surgery relies on identification of the bregma and lambda sutures...coordinates at bregma which allows you to quickly and reliably locate your injection site. We recently purchased...

...Human Mouse IgG2a 114490 Anti-Kirrel3, short and long [N321C/49R] Kirrel3, short and long Rat Mouse IgG2a... Rat Mouse IgG2a 149452 Anti-Kirrel3, short isoform 3 [N320/48R] Kirrel3, short isoform 3 Mouse Mouse ...

...CMV ALS Alicia Pickrell 131792 pHAGE-TBK1-C-FLAG-HA TBK1 STOP codon CMV ALS Alicia Pickrell 131889 pDONR221...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic Lateral...pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ALS Trina ...

...impenetrable by a needle, and must be able to close securely. Biohazard signs should be placed in all areas...

... Note: This step causes the bacteria to lyse, releasing their contents, including plasmid DNA, into solution...

...Engineering Browse the list of available plasmids related to synthetic biology below. If there are plasmids...