Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 335 results for: c rel

Showing: 201 - 220 of 335 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...and others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish...Zebrafish Xenopus References Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, Romero ...23287718 Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Côté J, Doyon Y. 2015. A Scalable Genome-Editing-Based...Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann ...years later, PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve...developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors directly...

  2. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L., & Baker, D. (2003)....John Jumper! The Baker Lab focuses on designing entirely new proteins, improving our understanding of how...

  3. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...predictable kinetics over 14 hours of expression in C. elegans embryos. However, this initial timer was ...timer could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus embryos. Of course, timers...of protein concentration. Calibration curves correlating this ratio to the total expression time can therefore...used to: Monitor dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle... situation where one wants to understand the relationship between the age of a cell, protein, or cellular...nascent tissues and the formation of limbs are correlated with changes in gene expression. In a developing...

  4. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ... a AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract...Deisseroth. (2014) Nat Protoc., 9, 1682-1697. Pubmed. C. Soderblom, D.-H. Lee, A. Dawood, M. Carballosa, et...major advances in light microscopy, particularly related to the neurosciences.  These developments in microscopy...microscopy) (16).  Both 3DISCO and CLARITY have relied on the use of transgenic mice in which a subpopulation... our understanding of the structure-function relationship of the central nervous system under normal and...

  6. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...construct. Reactions are often cycled between 37°C and 16°C to provide optimal reaction temps for both the...practices for assemblies of more than 10 modules often rely on two-step hierarchical approaches using different...pre-determined manner. If the original insert religates into its original vector, it will subsequently...

  7. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies/uL V = Volume of virus ...polymerase chain reaction Browse our blog posts related to viral vector protocols and tips Read all of ... by qPCR Browse our collection of viral vector related protocols Learn more about the Addgene viral service...

  8. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Most FP fusions for FRET studies are at the N- or C-terminus of your target protein, but even these modifications...can insert your protein of interest, resulting in a C-terminal fusion of a tetracysteine tag. In addition...for observed FRET efficiencies between 80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with...poly-histidine tag often used for protein purification is a reliable FRET acceptor labeling site when used in conjunction...

  9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...overhangs Cas13a (C2c2) 2x HEPN crRNA RNA 3' A, U, or C (not required by all orthologs) Specific RNA cleavage...spacer sequence and consists of a single A, U, or C base pair. In bacteria, once Cas13a has recognized...Class 2, candidate 1, 2, or 3). C2c1 and C2c3 are related to Cpf1, but they require both a tracrRNA and crRNA...

  10. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... epitope such as c-Myc, GFP or V5 can be used to tag the protein of interest on the C- or N-terminus. ...conformations and interactions. For difficult to release proteins, including those in the nucleus, more ...

  11. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...into an attL-containing entry vector.   Method C: Restriction cloning of a restriction enzyme fragment...attL-containing entry vector.   Figure 5: Method C to create an entry clone: Restriction cloning the ...their desired DNA sequences. Gateway technology relies on the two reactions described below: The BP Reaction...

  12. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...reactions, where a fraction of one of the four bases (A, C, T, or G) is supplied as a dideoxyribonucleoside triphosphate...regions of interest, or complex sequences such as G-C rich areas, secondary structures, or repetitive regions...widely used DNA sequencing technology. The process relies on “sequencing-by-synthesis,” where each base is...sequencing.  Prior to short-read sequencing, Addgene relied on Sanger sequencing for QC. We occasionally still...Second-generation sequencing”)  Short-read sequencing also relies on sequencing by synthesis, but modifies the original...

  13. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...agarose gel to confirm product presence, size, and relative quantity. End point PCR is most commonly used ...     References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic...reagents and several heating and cooling steps. PCR relies on heat resistant DNA polymerase from the thermophilic...

  14. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...8.2 kb, and in vivo transgene expression ceases relatively quickly due to immune response against AdV. Importantly...4. Mittereder, Nanette, Keith L. March, and Bruce C. Trapnell. "Evaluation of the concentration and bioactivity...

  15. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...Nicotiana benthamiana. N. benthamiana is a tobacco relative and a good model for plant disease because it ...assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLoS One. 2011...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...specificity of GFP expression for the indicated ssAAV5s. C) Quantifications showing high microglia transduction... microglia-targeting AAV plasmids here! Serrano, C., et al. (2023). Simple and Highly Specific Targeting...fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar...dCas9-VPR, prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various...

  18. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...these methods a try.  References:  Branon, Tess C., et al. "Efficient proximity labeling in living cells...1837. PubMed PMID: 30676735. Kim, D. I., Jensen, S. C., Noble, K. A., KC, B., Roux, K. H., Motamedchaboki...array of biological contexts.  Proximity labelling relies on the labelling of neighboring proteins within...interactions in vivo (Ge et al., 2019). This technique relies on an S. auerus transpeptidase sortase A variant...

  19. Neurodegeneration Research Collection

    Type
    Collection
    ...aggregation. Saha et al. Nat Commun. 2023 Feb 2. Study the C terminal domain of TDP-43 to better understand its... atlas for whole-brain neuronal identification in C. elegans. Yemini et al. Cell. 2021 Jan 7. Use a FRET-based...common disorders followed by a list of some of the relevant collections available at Addgene. Amyotrophic ...growing collection of general and neuroscience -related antibodies including: Anti-KChIP1 K+ channel [K55...diagnostics and treatments for Alzheimer’s disease and related disorders. The site curates information on useful...

  20. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 177559 Anti-c-Fos [N486/32R] c-Fos Human Mouse IgG2a 177560 Anti-c-Fos [N486/76R] c-Fos Human Mouse...IgG2a 177520 Anti-LRRK2/Dardarin, C-terminus [N241A/34R] LRRK2/Dardarin, C-terminus Human Mouse IgG2a 177521...glutamate receptor Mouse Mouse IgG2a 206619 Anti-c-Fos [N486/25R] c-Fos Human Mouse IgG2a 206620 Anti-Kv1.4 K...220411 LRRK2/Dardarin kinase, C-terminus [N241A/58R] LRRK2/Dardarin kinase, C-terminus Human Mouse IgG2a ...VAPA [N479/111R] VAPA Rat Mouse IgG2a 220414 c-Fos [N486/93R] c-Fos Human Mouse IgG2a 220415 Cavalpha2delta2...] N483/126 Rufy3 Mouse Mouse 182090 C-Fos scFv [N486/32] N486/32 C-Fos Human Mouse 182091 Mortalin/GRP75...84 scFv Rufy3 Mouse Mouse 190560 c-Fos scFv [N486/76] N486/76 scFv c-Fos Human Mouse 190561 BKbeta2 K+...
Showing: 201 - 220 of 335 results