We narrowed to 401 results for: caps
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TypeProtocol...contaminants with lower densities, including empty capsids. The 60% step serves as a cushion for genome-containing... number of contaminants in each fraction. * AAV capsid proteins VP1, VP2, VP3; M protein marker. Image...
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What is Polymerase Chain Reaction (PCR)
TypeProtocol...you are using a polymerase with proofreading capabilities. Note: See manufacturer’s instructions for specific... of the reaction mix. Its solvent and buffer capabilities provide a suitable environment for the DNA amplification... -
Western Blot
TypeProtocol...Microcentrifuge tubes BCA Assay, Thermo Fisher 23227 β-mercaptoethanol 4X protein loading buffer Precast SDS-PAGE ...sample for loading as follows: Add 10% v/v β-mercaptoethanol to the 4X protein loading buffer. Dilute 4X... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog Post...elements; resources on different types of vectors, capsids, and tools; and helpful information on everything... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog Post... We love the way the names Aavery, Addy, and Cappy play on viral vector terms. And, the name Kendall... -
Addgene's 20th Anniversary Party
TypeBlog Post...photos and slideshows shared some of the moments we captured over the years, including an album of Addgenie... -
The 12 Days of CRISPR: 2021
TypeBlog Post...CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth ... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog Post...: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj; Viviana Gradinaru; Tim... -
New Educational Resource: The Addgene Videos Page
TypeBlog Post...How-to videos Our How-To videos feature screen-capture footage to help you navigate Addgene’s vast online... -
Summer Fun at Addgene!
TypeBlog Post...haven’t seen our webinar “Navigating an Expanding Landscape for Engineered Systemic AAVs” with the director... -
Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6
TypeBlog Post...therapeutics. After a brief stint at the venture capital firm, Flagship Ventures, Koeris helped change Novophage's... -
2022 and 2023: Reflections and Looking Forward
TypeBlog Post...and a compelling future to plan in the changing landscape of science. We are therefore embarking on a strategic... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post...are not exposed to the vast non-academia career landscape and there is insufficient (or no) emphasis on ... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog Post...Precision Cloning Method with High Throughput Capability." PLoS ONE. 3(11): e3647 (5 November 2008). ... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog Post...the phage field, subscribe to their newsletter, Capsid and Tail. Additional resources on the Addgene ... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...nm, photoconverted with 405 nm light, and then captured in the red state after excitation at 561 nm. This... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post... for what we have accomplished. Starting with a recap of accomplishments, from learning new techniques... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post... mutation [3]. In the CRISPR era, the genomic landscape may be surveyed in unprecedented fashion. Targeted... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post... to their genes of interest, allowing for easy capture and detection of proteins and complexes, without... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog Post...AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg) capsids. Please be aware that this ordering process will...