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We narrowed to 215 results for: crispr cas9 expression plasmids

Showing: 201 - 215 of 215 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag...Species-Specific Expression If you want to drive expression of your favorite gene, you will need a plasmid with...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 ... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...

  2. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Plasmids Bas Teusink Lab Materials...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression Resources This comprehensive...proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs and tagging of genes...constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al...proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee et al., 2013), and are...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ...Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR Libraries...different plasmids. Plasmids within a given library have the same backbone, but they express or target...protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse our ... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled Libraries Jump To: Pooled Library...libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains a unique... others, notably pooled lentiviral plasmid libraries, the plasmids must first be used to make virus. This...infected cell receives only one plasmid. To make sure that every plasmid is adequately represented in the...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...different plasmid. In order to produce rAAV vectors, three plasmids are needed: Transfer plasmid (also known...the packaging plasmid. For a summary of all AAV plasmid components, see the AAV Plasmid Elements table...Optogenetics Guide . Browse optogenetics plasmids available at Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular...delivery of CRISPR/Cas9 elements, making it one of the most common methods for in vivo CRISPR-based genome...as cis plasmid) — containing the transgene of interest between the two ITRs. Packaging plasmid (also known...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...Production The three production plasmids (transfer, packaging, and helper plasmid) are transfected into cells...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. ...al., 2024). In MagIC-cryo-EM, target proteins (expressed as a GFP fusion in this proof-of-concept study...be used for applications beyond cryo-EM.   Find plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi...disease phenotype in an animal model. Find PE-eVLP plasmids here! The lab’s other recent deposit builds on...over the wild-type enzyme. Find optimized PASSIGE plasmids here!     Figure 3: Schematic of the PASSIGE...recombinase integrates a sequence from a donor plasmid containing a complementary site into the genome...showed that AAV-delivered CHARM could silence the expression of prion protein in mouse brains for at least...

  6. Adenovirus Guide

    Type
    Guide
    ...you generally need two plasmids: Shuttle plasmid (also known as transfer plasmid) — containing the transgene...shuttle plasmid. For a summary of all adenoviral plasmid components, see the Adenoviral Plasmid Elements...transgenes to express for a shorter period of time or when needing to package larger cargo. CRISPR-Cas9 Delivery...for recombination with the adenoviral plasmid. Adenoviral plasmid — containing the rest of the adenoviral...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...consists of two plasmids that eventually recombine to form one: Shuttle/transfer plasmid (e.g. pAdTrack...backbone plasmid containing the adenoviral genes necessary for virus production. The shuttle plasmid and the...

  7. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently... range of expression levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The... using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs... protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur... has deposited a collection of CRISPR arrays to modulate the expression levels of two targets independently...

  8. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ... in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab...Complementing this mutation by expressing this deleted portion, for example on a plasmid, creates a functional ...once in the plasmid backbone and another enzyme that cuts within the insert. If the plasmid contains the... PCR product if the plasmid contains the insert and no PCR product if the plasmid does not contain the...resources on the Addgene blog Get all the plasmid basics in our Plasmids 101 series Find more molecular biology...If you’re cloning a plasmid, you’ll need a way to find the needle in the haystack: the one perfect clone...clone that contains the plasmid you’re looking for out of the many cells that don’t. One way to begin ...

  9. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression in mammalian... CRISPR Interfere CRISPR Plasmids: Interfere...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...use a separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. Want more information...CRISPR plasmids for transcriptional repression of target genes. CRISPR...Interfere Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use ...

  10. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression in mammalian...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter...

  11. CRISPR Plasmids and Resources

    Type
    Collection
    ...preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used ...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed.... C. elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for ... Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for... parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...

  12. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated... tables below for CRISPR Transposase plasmids. Plasmids are available for expression in mammalian systems...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... CRISPR CASTs CRISPR...

  13. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas...the tables below for CRISPR nickase plasmids. Plasmids are available for expression in mammalian systems... CRISPR Nick CRISPR Plasmids: Nick...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...Nick Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...

  14. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression in mammalian... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully...CRISPR plasmids that create double strand breaks in DNA. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert... elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...

  15. Lentiviral Vector Guide

    Type
    Guide
    ...second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — contains...Packaging plasmid 1 — contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains...systems Packaging Plasmid One plasmid encoding gag , pol , tat , and rev Two plasmids, one encoding gag...Lentiviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ...separated into distinct plasmids for safety. For a summary of all lentiviral plasmid components, see the ...four, for third generation) plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest ...rev ; separated into two plasmids in third generation systems Envelope plasmid — contains packaging gene...
Showing: 201 - 215 of 215 results