Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 274 results for: crispr cas9 plasmids

Showing: 201 - 220 of 274 results

  1. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids are available...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should... CRISPR dCas9-FokI ...

  2. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR...evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants...

  3. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic...shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...Collections Brzezinski Lab CRISPR Collection Brzezinski Lab CRISPR Collection The Brzezinski lab investigates...involved in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several modifications...Schematic of the CRISPR Collection. Plasmids 159654 and 159655 are single guide expressing double stranded...

  4. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...organism.   It’s not an overstatement to say that CRISPR/Cas9 editing is one of the key tools now providing...plans and in particular, that they all share the plasmids created under this programs via Addgene. New organisms...clutch of eggs? Propagation is thus quite difficult. CRISPR genome editing in squid is possible nonetheless...reptiles at all. But new success is coming with CRISPR/Cas in lizards. The Menke Lab was able to make ...sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get transgenic animals. They...-Mediated Ovary Transduction of Cargo)  delivers Cas9 RNP to the arthropod germline by injection into ...

  5. Hot Plasmids - May 2022

    Type
    Blog Post
    ...The dCas9-SSAP system reports an efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...mutations. These plasmids contain catalytically inactive dCas9 and microbial SSAPs (dCas9-SSAP) which insert...need. If you'd ever like to write about a recent plasmid deposit please sign up here. Here's what you'...easily be applied to other systems.    Find BLITZ Plasmids here! Xiong, et al. eLife. 2021. https://doi.org...from Goertsen D, et al., 2022.  Find M-CREATE plasmids here!  Goertsen D, et al. Nature Neuroscience....

  6. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...consisted of three plasmids: the packaging, envelope, and transfer plasmids. The packaging plasmid carried a mutated... Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015... packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with...Addgene Blog Your Lentiviral Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out...Lentiviral vectors are the vector of choice for many CRISPR applications, and they’ve also had success in clinical...itself due to a few missing proteins. The envelope plasmid contained a viral envelope to dictate the tropism...

  7. CRISPR Plasmids - Plants

    Type
    Collection
    ...of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ... CRISPR Plants CRISPR Plasmids: ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...CRISPR expression plasmids that have been designed for use in plants. CRISPR...

  8. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3... or search the tables below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach and include ...plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter...

  9. CRISPR Plasmids - gRNAs

    Type
    Collection
    ... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome Editing...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... any of these gRNA plasmids and review the publication associated with each plasmid for more information...PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...gRNA Plasmids Please note that the following table lists both published and unpublished plasmids containing...be used with any CRISPR nuclease and function. Please see the publication or plasmid information page...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...

  10. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...our journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept...Kotton Learn How Plasmid Sharing Speeds Science Resources at Addgene.org Order Plasmids from Addgene! ...to engineer microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast...Blog Experimenting in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science...

  11. Important Considerations When Using AAVs

    Type
    Blog Post
    .... Currently, AAVs are being used to deliver CRISPR/Cas9 but have the potential to deliver other therapeutic...used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient...Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional Resources...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

  12. How Addgene Creates Content

    Type
    Blog Post
    ...knowledge changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in...Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website,...mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists ...

  13. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...every isolated plasmid sample and normalize each to fall within a 10 fold range. Plasmids that don't meet...A broader view of the whole plasmid To confirm the sequence of the plasmid, we examine three things: ...expected and do not affect plasmid function, we will update information on the plasmid’s page. Sometimes we’re...’re missing a piece of the plasmid There are regions of some plasmids that are particularly difficult ...some plasmids will not result in one complete, circular assembly. Most of the time, these plasmids are ...bottom of the plasmid page. Viral preps and pooled libraries In addition to regular plasmid sequencing,...sequence all plasmids in the pool but instead selectively sequence the variable portion of the plasmid in the...

  14. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...a 1st Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian...48-well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western ...; more recently we have also been using Addgene CRISPR vectors to generate cell lines with knockouts of...Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors ...

  15. Antibodies 101: Validation

    Type
    Blog Post
    ...RNAi to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate...gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large...interest endogenously and transfect them with a plasmid encoding your protein of interest. Then you should...target protein in a Western blot. You acquire a plasmid encoding your protein of interest, known as Gene... In parallel, you transfect cells with another plasmid not encoding this protein, which we will call Negative...band from the cells transfected with the other plasmid. Your antibody works! Multiple (Independent) Antibodies...Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ...

  16. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...RNAs.   RNAi vs. CRISPR: Complementary techniques It is fair to say that CRISPR has revolutionized ...A, Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes. Nat Biotechnol...our CRISPR blog posts Resources on Addgene.org Find tools for mammalian RNAi Browse all plasmids for...unprecedented ways. With the meteoric rise and diversity of CRISPR technologies, one might incorrectly relegate RNAi...straightforwardness of RNAi is unmatched—unlike CRISPR experiments that require exogenous protein, successful...only requires the presence of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt ...function at the level of DNA, thereby limiting CRISPR’s effectiveness at high copy numbers and genomic...

  17. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...min. Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast!  MarathonRT...’s lab at Yale University recently deposited a plasmid for expressing a novel reverse transcriptase (RT...polymerase processivity. Please find the MarathonRT plasmid here! Zhao C, et al. RNA. 2018 PubMed PMID:29109157... cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP nanobody fused with...

  18. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying...search the tables below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR... CRISPR Purify CRISPR: Purify Genomic ...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  19. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic...CRISPR expression plasmids that have been designed for use in parasites. CRISPR... CRISPR Parasites CRISPR Plasmids...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and ...than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  20. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...or search the tables below for CRISPR RNA targeting plasmids. Plasmids are available for expression in...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should ... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In ...
Showing: 201 - 220 of 274 results