We narrowed to 435 results for: erg
-
TypeBlog Post... to tell if the dependent variables worked synergistically, antagonistically, were additive, etc.). ANCOVA... The Bonferroni Correction and the Benjamini-Hochberg procedure are designed to reduce false positive...
-
A History of Genome Engineering in Popular Culture
TypeBlog Post...In the 1960s, Har Gobind Khorana, Marshall W. Nirenberg, and Robert W. Holley cracked the genetic code... a renaissance in the field of health sciences, energy, and agriculture, its portrayal in popular fiction... -
Antibodies 101: Validation
TypeBlog Post...Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...Rensselaer Polytechnic Institute. He also studies energy markets in his spare time. You can follow him on...are not present at the lab bench," Liu, a UM undergraduate, challenged us. I worried the march would be... -
Tetracycline Inducible Expression
TypeCollection...Tet-On plasmid for differentiating iPSCs into glutamatergic neurons via human NGN2 expression. See more ... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...generations of optimization before the field begins to converge on a set of standard tools. Additionally, aspiring...Base Editing. Advanced Science (Weinheim, Baden-Wurttemberg, Germany), 9(26), e2202957. https://doi.org/10.1002... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...hypopigmentation, thus marking cells that have undergone genomic modification. In this system, Cas9 must...plasmids from this paper at Addgene. 8. Xie, Kabin, Minkenberg, Bastian, & Yinong Yang. “Boosting CRISPR/Cas9... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...editor proteins, but found that the mutations that emerged depended strongly on the target edit (Doman et .... E., Holden, K., Ma, H., Xie, J., Gao, G., … Gootenberg, J. S. (2023). Drag-and-drop genome insertion... -
Antibody Validation for Flow Cytometry
TypeBlog Post...Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm, D. L., Rodriguez, H., Hiltke... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...genetic material. The mitochondria produces the energy cells required for chemical reactions. Labeling... -
Using AAV for Neuronal Tracing
TypeBlog Post...in only specific cell types, for example only dopaminergic neurons, neuronal tracing has the added benefit...798–810. PubMed PMID: 92475. Taymans, J. M., Vandenberghe, L. H., Haute, C. V., Thiry, I., Deroose, C.... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...compartment, you may want to try an SDS-containing detergent like RIPA. You can try a few different lysing ...background noise is high, you can consider adding a detergent like Tween-20 to your washes — although this can... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...sgRNA-scaffold 47912 Worm AflII none S. pyogenes Sternberg pJW1311 61254 Worm none, fusion PCR none S. pyogenes... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...mitochondrial degradation in vitro and in vivo. Merged green and red channel fluorescence images of C2C12...effectively cuts the target sequence, the plasmid undergoes HR or SSA to reconstitute functional GFP, and ... -
Antibody Guide
TypeCollection...against parasites and is responsible for driving allergic reactions such as anaphylactic shock Monomer with... -
27 Hot Plasmids from 2016
TypeBlog Post... et al. Science. 2013. PubMed PMID: 24136966 Nyerges, et al. Proc Natl Acad Sci USA. 2016. PubMed PMID...Zurovec pBlue-TAL Nathan Lawson pJDS Series Superglue proteins with SpyTag/SpyCatcher and SnoopTag/SnoopCatcher... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog Post...Toolbox Hot Plasmid 2: AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new... -
The 12 Days of CRISPR
TypeBlog Post...day of CRISPR, Addgene gave to me: RADARS from Gootenberg, Chen, and Abudayyeh🎵On the 9th day of CRISPR... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog Post...Hardware? Author: Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post...dvpr (lentiviral) or pUMVC (MuLV retroviral). Bob Weinberg pMD2.G VSV-G envelope expressing plasmid Didier...