We narrowed to 412 results for: his

...to fluorophores. Examples are the FlAsH tag, the His tag and several others. Another peptide-based strategy...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

... lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative regulators...of such targets include transcription factors, histones, and DNA repair enzymes. Note that when using ...stepwise decrease in fluorescence intensity in a (histogram) flow plot (Figure 4b). Through this method, differences...

... lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative regulators...respectively. H, A, and W describe the shape of a histogram, which graphically represents the time and intensity...graph of photocurrent vs. time. The resulting histograms is described through maximum current (H), the...

...blogger Jacob Lazarus from Harvard. Jake received his MD and a PhD in Cell and Molecular Biology from the...

... a mentor with international experience provides his/her mentees with a broader, first-hand vision of ...

... Associate Product Marketing Manager at Promega. His work focuses on commercializing their cell and protein...

...Scientific reproducibility As Jeffrey Flier points out in his talk (1:24 - 29:31), there are a variety of ways ...

...N terminal); 6xHis (N terminal); 2xNLS (C terminal) 47327 pET-28b-Cas9-His 6xHis (C terminal);...pET-Cas9-NLS-6xHis 6xHis (C terminal); NLS (C terminal) 62934 pET-NLS-Cas9-6xHis 6xHis (C terminal...

...of CRISPR in 2013 (Cong et al., 2013)! Haoyi got his first exposure to CRISPR when he used this new technology...

...TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized knowledge to help researchers identify...

...likely to lead to scores. That player doesn’t trust his teammates; the teammates don’t trust him. This team...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...

... still infect the researcher and insert into her/his genome - this is a biosafety risk Infects dividing...

... Associate Product Marketing Manager at Promega. His work focuses on commercializing their cell and protein...Throughout their history, fluorescent dyes have enabled the visualization of both organic tissues and...technologies in fluorescent dyes/ligands Throughout the history of cell biology, fluorescent labeling techniques...fluorescent protein Fluorescent Proteins 101: A History of Fluorescent Proteins ...

... lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative regulators...

...calcium". Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc) on your insert. Use the drop-down...

...Centre for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing CRISPR/Cas9...

...Stroik and Meghan Rego, with thanks to Rob Hurt for his expertise and input.  References and resources References...

...expression measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...population (pink histogram) compared to the WT cells (green histogram). The unfilled histograms show cells ...

...Additionally, I would like to thank Le Cong for all his hard work creating amazing resources for the scientific...your HR by doing Restriction Fragment Length Polymorphism (RFLP) (see Figure 4 of the Cong et. al (2013...

...Mammalian TAG Abhishek Chatterjee 217365 pIDTSmart-CMV-PLRS1 LeuRS E. coli Mammalian Abhishek Chatterjee ...PylRS M. barkeri azidolysine (AzK) Mammalian TAG Abhishek Chatterjee 217364 pAcBac1-BacMam-4x-PyOtR PyOtR...-h14 (S8A, V144G, V146C) E. coli Bacterial TGA Abhishek Chatterjee 218765 pEvoltac EcW-TGA-h9 TrpRS-h9...TrpRS-h9 (S8A, V144S, V146A) E. coli Bacterial TGA Abhishek Chatterjee 218766 pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA...D182S, F183M, L186A, D265R) E. coli Bacterial TAG Abhishek Chatterjee 218767 pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA...D182C, F183Y, L186C, D265R) E. coli Bacterial TAG Abhishek Chatterjee 220800 EF1a-L274GMmMetRS-T2A-mCherry...coli 5-Hydroxytryptophan (5-HTP) Mammalian TGA Abhishek Chatterjee 228782 pAcBac1-EcTyr-TAG TyrRS E. coli...