...to fluorophores. Examples are the FlAsH tag, the His tag and several others. Another peptide-based strategy...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

... a mentor with international experience provides his/her mentees with a broader, first-hand vision of ...

..., or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...methods such as immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use... they only have one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can... can refer to both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (...

..., or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...methods such as immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use... they only have one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can... can refer to both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (...

...N terminal); 6xHis (N terminal); 2xNLS (C terminal) 47327 pET-28b-Cas9-His 6xHis (C terminal);...pET-Cas9-NLS-6xHis 6xHis (C terminal); NLS (C terminal) 62934 pET-NLS-Cas9-6xHis 6xHis (C terminal...

...Scientific reproducibility As Jeffrey Flier points out in his talk (1:24 - 29:31), there are a variety of ways ...

...of CRISPR in 2013 (Cong et al., 2013)! Haoyi got his first exposure to CRISPR when he used this new technology...

...TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized knowledge to help researchers identify...

...likely to lead to scores. That player doesn’t trust his teammates; the teammates don’t trust him. This team...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...

... still infect the researcher and insert into her/his genome - this is a biosafety risk Infects dividing...

... lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative regulators...

... Associate Product Marketing Manager at Promega. His work focuses on commercializing their cell and protein...Throughout their history, fluorescent dyes have enabled the visualization of both organic tissues and...technologies in fluorescent dyes/ligands Throughout the history of cell biology, fluorescent labeling techniques...fluorescent proteinFluorescent Proteins 101: A History of Fluorescent Proteins  ...

...calcium". Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc) on your insert. Use the drop-down...

...Stroik and Meghan Rego, with thanks to Rob Hurt for his expertise and input.  References and resources References...

...Centre for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing CRISPR/Cas9...

...expression measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...population (pink histogram) compared to the WT cells (green histogram). The unfilled histograms show cells ...

...p15TV-L EF456736 Hexahistidine tag with TEV cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV...GST-tag and hexahistidine tag with Thrombin cleavage, KanR 26096 pET28-MHL EF456735 Hexahistidine tag with...26117 pNIC-CH EF199843 Hexahistidine tag, AmpR 26103 pNIC28-Bsa4 EF198106 Hexahistidine tag with TEV cleavage...EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106 pNH-TrxT GU269914 Hexahistidine tag, thioredoxin...GU452710 Hexahistidine tag , Z-basic, TEV cleavage, AmpR 26108 pFB-LIC-Bse EF199842 Hexahistidine tag with...TEV cleavage, AmpR 26094 pET28a-LIC EF442785 Hexahistidine tag with Thrombin cleavage, KanR 26101 pET28GST-LIC...ptac promoter, AmpR 26099 pFBOH-LIC EF456740 Hexahistidine tag with Thrombin cleavage, Bac-to-Bac Baculovirus...

...PylRS M. barkeri azidolysine (AzK) Mammalian TAG Abhishek Chatterjee 217364 pAcBac1-BacMam-4x-PyOtR PyOtR...PyOtR M. mazei azidolysine (AzK) Mammalian TAG Abhishek Chatterjee 218764 pEvoltac-EcW-TGA-h14 TrpRS-h14...TrpRS-h14 (S8A, V144G, V146C) E. coli Bacterial TGA Abhishek Chatterjee 218765 pEvoltac EcW-TGA-h9 TrpRS-h9 (... (S8A, V144S, V146A) E. coli Bacterial TGA Abhishek Chatterjee 218766 pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA...D182S, F183M, L186A, D265R) E. coli Bacterial TAG Abhishek Chatterjee 218767 pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA...D182C, F183Y, L186C, D265R) E. coli Bacterial TAG Abhishek Chatterjee 220800 EF1a-L274GMmMetRS-T2A-mCherry...incorporate other Uaas (derived from EcNR1 strain) Abhishek Chatterjee 218769 ATMW-BL21 Removed endogenous...

...-A major histocompatibility complex, class I, A FLJ26655, HLAA HLA-B major histocompatibility complex,...major histocompatibility complex, class II, DM beta D6S221E, RING7 HLA-DOA major histocompatibility complex...HLA-DOB major histocompatibility complex, class II, DO beta DOB HLA-DPA1 major histocompatibility complex, ...major histocompatibility complex, class II, DQ alpha 2 HLA-DXA HLA-DQB1 major histocompatibility complex...major histocompatibility complex, class II, DR alpha HLA-DRA1 HLA-DRB1 major histocompatibility complex... HLA-G major histocompatibility complex, class I, G MHC-G HLA-H major histocompatibility complex, class...protein-coupled receptor 77 C5L2, GPF77 HTN3 histatin 3 HIS2, HTN2, HTN5 IL8 interleukin 8 CXCL8, GCP-1,...

...206528 Anti-Histone H3 pThr11 [N123/19R] Histone H3 pThr11 Rat Mouse IgG2a 206529 Anti-Histone H3 pThr11...28R] HCN3 Mouse Mouse IgG2a 177500 Anti-6xHis [N144/14R] 6xHis Mouse IgG2a 177501 Anti-TrpV3 [N15/4R] ...Pan-Gamma-protocadherin-A Mouse IgG2a 182106 Anti-Histone H3.3 [N158/28R] Histone H3.3 Rat Mouse IgG2a 182107 Anti-LRRTM2...Mouse Mouse IgG2a 206552 Anti-Histone H4-dimethyl-Arg3 [N309A/21R] Histone H4-dimethyl-Arg3 Human Mouse...musculus (mouse) IgG2b 227037 6xHis recombinant mouse monoclonal antibody. 6xHis Chimera: M. musculus (mouse... Mouse IgG2a 188214 Anti-GST [N100/13R ] GST Schistosoma japonicum Mouse IgG2a 188215 Anti-TrpC7 [N64A...pThr11 [N123/48R] Histone H3 pThr11 Rat Mouse IgG2a 206530 Anti-Haspin/GSG2 [N128A/2R] Haspin/GSG2 Human Mouse...