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We narrowed to 1,075 results for: ica;

Showing: 201 - 220 of 1075 results

  1. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...two types of replicate controls: technical and biological. In general, technical replicates can be thought...of the independent variable. Replicate controls: technical and biological Reliable results are reproducible...independent and are typically derived from one source. Conversely, biological replicates ARE independent ... more in depth information. Technical replicates An example of a technical control is the transfection...independent variable. Biological replicates In any given set of experiments, the biological controls are more...results. Typically, plasmid-based experiments employ transfection, negative, positive, and replicate controls...thought of as "reproducibility controls". Biological replicates are what makes up your sample size (aka ...

  2. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...non-bench research career such as law, medical/technical writing, clinical, regulatory, product development,...specialty occupation" as requiring theoretical and practical application of a body of highly specialized ...Further, this post may contain technical inaccuracies or typographical errors.  No action should be taken...18, 2017, President Trump signed a "Buy American, Hire American" Executive Order which sets broad policy... industry such as technology transfer, communications, medical writing, compliance, core facility management...received 116,224 employment based green card applications and approved 91,023 during the first 3 quarters...options discussed in this post benefit from the applicant thinking about the process early, keeping good...

  3. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...as synthesis modifications when purchasing your gRNA. Pro tip: modifications are typically incorporated...switches. Stabilizing gRNA modifications  Sugar phosphate backbone modifications The recognition sequence...phosphorothioates with 2’-O-methyl modifications, proved more stability than just one modification alone (Hendel, et al...modified) for optimal stability and efficacy.  Base modifications What is more stable than RNA? DNA! ...stabilizing modifications listed above were developed before CRISPR-Cas9, for use in applications like siRNA...oligonucleotides. Some of these synthetic modifications, specifically ones which do not contain a 5’ triphosphate...your next CRISPR experiment.    Chemical RNA modifications to improve stability.   On-off switches...

  4. Engaging with science and society at pgEd

    Type
    Blog Post
    ...in science communication or policy, being able to communicate your work to a non-technical audience is...knowledge and applications generated from this research. At both a practical and ethical level, scientists...benefits as well as the personal, social and ethical implications of genetics. In these conversations, pgEd...training beyond the biological sciences, as well as to acquire leadership and communication experience. These...science policy and science communication A career in science communication or policy requires broad interest... in our field of interest. We often communicate in very technical and jargon-filled language in academic.... Also seek out opportunities to communicate science to non-technical audiences. Many universities or ...

  5. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...illumination on biochemical processes that are crucial in the world of biological research, medical therapy, ...components, typically a protein of interest. Fluorescent dyes and ligands vary in their chemical structure...the affinity tag they typically bind to. Stability of fluorescence: For applications that involve ranges...recruitment to receptors, indicating their agonistic nature. The potency and efficacy of these ligands can ...their product descriptions, or technical manuals. Specific applications and matching dye/ligand choices... in biological research. This does make finding the right dye for your experiment more complicated. We...ligands with G protein coupled receptors. In Biochimica et Biophysica Acta - Biomembranes (Vol. 1838, Issue ...

  6. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... norvegicus genes! Mexican axolotl (Ambystoma mexicanum) Ambystoma mexicanum, aka the axolotl. Image...essential aspect of biological research that has defined our understanding of biological processes and developments...studying genetic modification for their ease of manipulation and their ability to be replicated. Because E....providing insight into some of the most significant and fundamental biological processes. Many of their genetics...tools in developmental and biomedical research.  Xenopus, aka African frog. Image from Holger Krisp...Advances on Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306...Organism. News-Medical. Retrieved on September 18, 2020 from https://www.news-medical.net/life-sciences...

  7. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...the grant application process. When my PhD advisor first asked me to write a grant application due the ...pursue. Proposal writing: Improves technical writing and communication skills, Elucidates the big picture...information required for a successful grant application. It typically includes overview information, key dates...the next round of applications. Step 5: Keep writing! Each written grant application builds upon the ... project grant application" by the NIH Still want more information on the application process? What types...Research at universities typically requires funding from a variety of government and academic institutions...advisor alone applies for these competitive grant applications; however, in some cases, your advisor may choose...

  8. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...Other than a theoretical maximum value, physical titers don’t necessarily give any indication of the infectious... red) report physical titer, which is measured by qPCR.   Physical titer is typically calculated by... you? Physical titer: the concentration of viral particles containing viral genomes. Physical titers ...qPCR or other DNA quantification methods - see below), since each viral particle typically contains one viral...facilities report the physical titer of a viral solution (Figure 2). Because physical titers are used for...titer values. Finally, because physical titer does not give an indication of infectious titer, consider...that can transduce cells. Infectious titers are typically quantified by cell transduction assays. Wild-type...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... of the American Chemical Society 123.48 (2001): 12123-12125. Journal of the American Chemical Society...discovery–a review of theoretical aspects and recent applications." Current Chemical Genomics and Translational...originally developed to treat heavy metal intoxication in chemical warfare [Vilensky & Redman, 2003]). Most...al. "Designing logical codon reassignment–Expanding the chemistry in biology." Chemical Science 6.1 (2015... of a biarsenical− tetracysteine motif determined by NMR spectroscopy." Journal of the American Chemical...laboratory, then FRET experiments are a viable biophysical path to answers. What is FRET and what's it...Fluorescence resonance energy transfer (FRET) is a biophysical interaction between two fluorophores. When the...

  10. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...underlying all communications efforts.  Scicomm definitions (as used by Addgene)    Science communication, or science...time engaging with science communication efforts.  Scientific communication or scientific writing is peer...to note that it is the actual communication itself, not the communication method, that determines if something...scientific or science communication. A Tweet is still considered scientific communication if the language is...Series, where we’ll be talking about science communication, best practices in the field, and even getting...getting into some active science communication research.   This summer, we’re making time to talk about ...about my favorite thing - science communication (scicomm)! We have so many fun topics planned for this summer...

  11. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...epigenome (the sum of epigenetic modifications across the genome) has been implicated in disease states, and targeting...DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety of epigenetic...Preps for Epigenetic Modification Why use epigenetic modifiers? Epigenetic modification is certainly not ...Nature Communications 8: . https://doi.org/10.1038/ncomms16026 Find plasmids from this publication at Addgene..., 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional layer of control over gene...efficient. In general, epigenetic chromatin modifications are correlated with alterations in gene expression...mechanisms remain unclear. Today, targeted epigenetic modification at specific genomic loci is possible using CRISPR...

  12. Plasmids 101: Protein tags

    Type
    Blog Post
    ...or DYKDDDK 1 Affinity and Purification Good for antibody-based purification; has inherent enterokinase...site GST Large Protein 26 Purification and Stability Good for purification with glutathione; protects...used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification Very small...GKPIPNPLLGLDST   1.4 Affinity and Purification Good for antibody-based purification Combo and cleavage tags...increasingly common. Tandem affinity purification (TAP) once referred specifically to a combo tag comprised of ...multitude of uses including (but not limited to) purification, detection, solubilization, localization, or...overexpress and purify proteins can face any number of technical challenges depending on their protein of interest...

  13. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...differing in pigmentation." Biochemical and biophysical research communications 78.1 (1977): 237-243. PubMed...one that can be achieved using pharmacological or electrophysiological methods (Deisseroth, 2011; Mei ...recombination. Purification and properties of the Cre recombinase protein." Journal of Biological Chemistry...Edward S., et al. "Millisecond-timescale, genetically targeted optical control of neural activity." Nature ..., et al. "Next-generation optical technologies for illuminating genetically targeted brain circuits." ...and Jinfang Zhu. "Immunologic applications of conditional gene modification technology in the mouse." Current... R. Thompson. "Overview on research and clinical applications of optogenetics." Current protocols in pharmacology...

  14. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... construct for every application. Plus, the photophysical properties of chemical dyes provide many advantages...Few Complicated Non-natural amino acids 1 Various Many Complicated   Applications Organic... W. (2011). Chemical Tags for Labeling Proteins Inside Living Cells. Accounts of Chemical Research, 44...If you take a look in a typical lab freezer, you’ll probably find lots of different plasmids containing...new green fluorescent protein, some affinity purification tags, another version with that same red fluorescent...substrate. If the substrate was linked to, say, a chemical dye or other functional ligand, your protein of...advantages over fluorescent proteins, opening up new applications.   Figure 1: Live-cell labeling strategies...

  15. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...Spatially Confined Persistent Diradical Emitter. Journal of the American Chemical Society, 145(25), 13615–13622... tools for familiar applications. But how does one invent a tool for applications that don’t even exist...organisms) are opaque, which makes most biomedical applications of these tools difficult or impossible....of developing new microscopy tools for biological applications across a wide range of topics. He embraces...., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification of magnetic field effects relevant to avian...phenomenon, improve the tool, and develop new applications for magnetoresponsive proteins. Motivated by...tool with magnets would enable new research and applications that don’t yet exist, but it wasn’t clear that...

  16. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...of Various Motifs is Critical for Initiation of Replication. Journal of Biological Chemistry, 273(14), ...to utilize diverse host replication machinery. For instance, the ori typically contains iterons that serve...ori2, ori3) and replication initiation proteins (RepA, RepB) necessary for replication in various hosts...the ori and allow for replication. However, if plasmids have their own replication machinery such as Rep...different hosts that a plasmid can transfer to, replicate in, and persist in. Some plasmids have a narrow... while others have a broad host range and can replicate and stably maintain the genes they carry among.... These features are: The plasmid origin of replication (ori) contains structural elements that are versatile...

  17. Typing CRISPR Systems

    Type
    Blog Post
    ... your own research! Classification Structure Principles of CRISPR classification While CRISPR systems... CRISPR-Cas systems. Philosophical Transactions of the Royal Society B Biological Sciences, 374(1772),...Shakespeare for the first time. The discovery and application of CRISPR-Cas9 kicked off over a decade of frenzied...properties. This led to a need to develop a classification system for the array of CRISPR technologies...technologies. Learning the general principles of this classification system will help you gain a deeper appreciation...multi-pronged analysis to sort effectively. The current classification system relies on a combination of sequence ...a similar function. After analysis, CRISPR classifications were placed into a fairly simple hierarchy:...

  18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short genetically encoded tetracysteine...systems consist of a genetically encoded enzyme variant tag that reacts specifically and irreversibly with... as intact tissue. Some chemical labeling strategies such as the bisarsenical dye (Figure 1a) and FAP ...Ronald T. Raines. "Bright ideas for chemical biology."ACS chemical biology 3.3 (2008): 142-155. PubMed... at the Janelia Research Campus, Howard Hughes Medical Institute. Chemistry is dead, long live chemistry...fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly, cell biologists were no longer...synthetic fluorophores. Add a dash of DNA with an electrical jolt and cells become perfectly capable of synthesizing...

  19. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...Retroviruses are not considered safe for clinical applications.     Lentiviral: Lentiviral vectors infect...lentiviral delivery is not considered safe for clinical applications. Excisable Cre-lox lentiviral vectors:... to reprogram. Basically, there’s no universal method that can handle all applications of iPSCs, but there...generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming... to verify there’s no genomic integration Replicating EBNA1 episome Non-integrating Medium Medium ...creates heterogeneous iPSC cell lines, which can complicate comparisons made between lines. Incomplete silencing...unavoidable heterogeneity between clones. This can complicate comparisons made between samples, i.e. transgene...

  20. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ... (enChIP) using CRISPR." Biochemical and biophysical research communications 439.1 (2013): 132-136. PubMed...affinity purification with an anti-Cas9 antibody. These different tag systems and affinity purification schemes...26848818. Fujita, Toshitsugu, et al. "Identification of physical interactions between genomic regions ...genomic alterations. These studies utilize a catalytically inactive form of Cas9 known as dCas9 (Jinek ...interest targeted by a gRNA. CRISPR-mediated purification of a specific genomic region In 2013, the Hodaka...Subsequent mass spectroscopy studies allowed for the identification of proteins associated with the IRF-1 locus...steps (Fujita et al., 2013). CRISPR-mediated purification of a specific genomic region alleviates these...
Showing: 201 - 220 of 1075 results