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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 658 results for: otos

Showing: 201 - 220 of 658 results

  2. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology 115: . https://doi.org/10.1002...

  3. Microbiology Resources

    Type
    Collection
    ... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains...microbe of interest, including bacteria, viruses, protozoa, fungi, and more. Plasmid...including bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids below for the species...Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica Leishmania sp. Plasmodium... page for CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast CRISPR...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...hydroxytryptamine (serotonin) receptor 3B 5-HT3B HTR3C 5-hydroxytryptamine (serotonin) receptor 3, family...RIFLE, TAL LTA lymphotoxin alpha (TNF superfamily, member 1) LT, TNFB, TNFSF1 LTB lymphotoxin beta (TNF superfamily...member 1 CD94 LGMN legumain AEP, LGMN1, PRSC1 LTA lymphotoxin alpha (TNF superfamily, member 1) LT, TNFB, TNFSF1... RNase A family, 2 (liver, eosinophil-derived neurotoxin) EDN, RNS2 ROBO1 roundabout, axon guidance receptor...3 HIS2, HTN2, HTN5 HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3...family member C - HTR3D 5-hydroxytryptamine (serotonin) receptor 3 family member D MGC119636, MGC119637 ...MGC119637 HTR3E 5-hydroxytryptamine (serotonin) receptor 3, family member E 5-HT3c1, MGC120035, MGC120036, MGC120037...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...contains many unique protospacer sequences that have homology to foreign DNA. Protospacers are separated by...Engineering You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR...contain a species-specific sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds...sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly after where the...target RNA rather than DNA, sometimes requiring a protospacer flanking sequence (PFS). In bacteria, Cas13 targeting...further develop the CRISPR toolkit by posting lab protocols , providing tips from experts in the field, and...

  6. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...you’ve gained experience working with similar protocols, and look - they’re seeking someone who has, to...Maybe there was a time you noticed an issue with a protocol and you took it upon yourself to fix the issue...you are in charge of completing many meticulous protocols or tasks, you’ve probably developed an attention...

  7. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...and ecology can make for beautifully compelling photos and videos, while molecular, micro, and cell biology...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...context via still images. Some things cannot be photographed or need text to properly contextualize. Graphics...

  9. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...Learn our protocol for AAV titration by qPCR Browse our collection of viral vector related protocols Learn...Browse our blog posts related to viral vector protocols and tips Read all of our viral vectors blog posts...

  10. Brain Initiative Collection

    Type
    Collection
    ...pAAV-CAG-iSeroSnFR-Nlgn Fluorescent reporter for serotonin (dendrite localization tag) Lin Tian 135420-AAV1...parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...frame with EGFP under control of human synapsin1 promotor. Ofer Yizhar 198513-AAV5 pAAV_hSyn-PdCO-EGFP-WPRE...frame with EGFP under control of human synapsin1 promotor. Ofer Yizhar 198516-AAV1 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination Ofer Yizhar ...

  11. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...them. DNA or RNA is pulled through the pore via motor proteins. When each nucleotide passes through the...Next‐Generation sequencing Technologies. Current Protocols in Molecular Biology, 122(1). https://doi.org/... Molecular Biology Reference Sequence Analyzer Protocol: Sequence Analysis of a Plasmid ...

  12. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...producing vectors from plasmids — including the protocols we use in our lab — being able to order high quality...educational resources, from eBooks to guides to protocols, which was why I was so excited to delve into ...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology, 115, 31.5.1-31.5.13. https...

  13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can... target sequence has been modified!  The exact protocol may vary depending on your specific experiment...

  14. COVID-19 Resources

    Type
    Collection
    .... Other Resources Protocols Coronavirus Method Development Community at Protocols.io (Link opens in a ...new window) Addgene's Protocol for RNA Extraction Without A Kit Bloom lab - Protocol and Reagents for Pseudotyping...linked to collections of open-access articles, protocols, and other resource collections related to COVID... Spike Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses (DOCX, 184 KB) Research Articles...

  15. CRISPR Guide

    Type
    Collection
    ...edition) CRISPR software and depositor protocols Video protocol for genomic deletions in mammalian cell...target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG)...can increase the risk of off-target cutting or cytotoxicity, but naturally-occurring Acr (Anti-CRISPR) proteins...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...before use (Figure 8C), following the amplification protocol specified by the depositing lab. After amplification...The translated codons that make up a gene PAM P rotospacer A djacent M otif; Sequence adjacent to the target...engineering using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A....

  16. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...using primers (with the desired mutation) in a PCR protocol that amplifies the entire plasmid template. The...simply following the polymerase manufacturers’ PCR protocol. Be sure to adjust the extension time to match... on Addgene.org Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment...

  17. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...PhosTag gels as described above. Reagents and protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial...Addgene # 68307 For further technical reference and protocols for use of this system, please reference ‘Rinehart...Western blotting using Phos-tag containing gel." Nat. Protoc. doi 10 (2008). Journal. Resources on the Addgene...

  18. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...genome with two-step allelic exchange." Nature protocols 10.11 (2015): 1820. PubMed PMID: 26492139. PubMed...bacteria using homologous recombination." Current protocols in molecular biology 106.1 (2014): 1-16. PubMed...molecular biology reference Watch molecular biology protocol videos ...

  19. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...protein characteristics such as the brightness, photostability, maturation time, and acid sensitivity of various...sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated...PMC490022. Shaner, Nathan C., et al. "Improving the photostability of bright monomeric orange and red fluorescent...

  20. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ... (Cai et al., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red)..., expect to see systems that use newer, more photostable fluorophores and that can be combined with new...Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson, Alexander...
Showing: 201 - 220 of 658 results