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We narrowed to 660 results for: otos

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Showing: 201 - 220 of 660 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...producing vectors from plasmids — including the protocols we use in our lab — being able to order high quality...educational resources, from eBooks to guides to protocols, which was why I was so excited to delve into ...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology, 115, 31.5.1-31.5.13. https...

  2. Optogenetics AAV Preps

    Type
    Collection
    ...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Karl Deisseroth...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Karl Deisseroth...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent 1, 5 Karl... 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive 8 Karl Deisseroth... pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Karl Deisseroth...pAAV-nEF-Con/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Cre and Flp dependent...-Coff/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Flp dependent 8 Karl ...

  3. Ras Pathway

    Type
    Collection
    ...Serine/threonine kinases: A-Raf proto-oncogene B-Raf proto-oncogene Raf-1 proto-oncogene RAL RALA RALB v-ral...substrate JUN Jun proto-oncogene KSR KSR1 KSR2 Kinase suppressor of ras MDM2 MDM2 proto-oncogene MEK MAP2K1...Mitogen-activated protein kinase ETS ETS1 ETS2 ETS proto-oncogene, transcription factor EXOC EXOC1 EXOC2 ...Mitogen-activated protein kinase kinase MET MET proto-oncogene, receptor tyrosine kinase MLST8 MTOR associated... coiled-coil containing protein kinase ROS1 ROS proto-oncogene 1, receptor tyrosine kinase RPS6KA RPS6KA1...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 ...amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols 2013). SpCas9 Cas9...Website You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9...co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9...insertion of guide sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing...cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors for...PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc . 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143...

  5. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...genome with two-step allelic exchange." Nature protocols 10.11 (2015): 1820. PubMed PMID: 26492139. PubMed...bacteria using homologous recombination." Current protocols in molecular biology 106.1 (2014): 1-16. PubMed...molecular biology reference Watch molecular biology protocol videos ...

  6. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...protein characteristics such as the brightness, photostability, maturation time, and acid sensitivity of various...sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated...PMC490022. Shaner, Nathan C., et al. "Improving the photostability of bright monomeric orange and red fluorescent...

  7. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ... (Cai et al., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red)..., expect to see systems that use newer, more photostable fluorophores and that can be combined with new...Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson, Alexander...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...using primers (with the desired mutation) in a PCR protocol that amplifies the entire plasmid template. The...simply following the polymerase manufacturers’ PCR protocol. Be sure to adjust the extension time to match... on Addgene.org Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment...

  9. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...PhosTag gels as described above. Reagents and protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial...Addgene # 68307 For further technical reference and protocols for use of this system, please reference ‘Rinehart...Western blotting using Phos-tag containing gel." Nat. Protoc. doi 10 (2008). Journal. Resources on the Addgene...

  10. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...or multicolor imaging. Photoactivatable iRFPs can be turned on by non-phototoxic far-red light and can ... imaging using far red, near-infrared, and photoactivatable fluorescent proteins. Plasmid...Proteins: In Vivo Imaging Far-Red Near-Infrared Photoactivatable Additional Resources Fluorescence microscopy...proteins are derived from bacterial phytochrome photoreceptors. Spectrally distinct fluorescent iRFP variants...pmiRFP720-N1 iRFP720 702 720 5.8 piRFP720-N1 Photoactivatable Protein Excitation (nm) Emission (nm) Activation...

  11. Genetic Code Expansion

    Type
    Collection
    ...Kensaku Sakamoto 197099 pIYN3 TyrRS M. jannaschii halogenated tyrosines Bacterial TAG Kensaku Sakamoto 197100...Kensaku Sakamoto 197101 pCDF-Az TyrRS M. jannaschii azidophenylalanine Bacterial TAG Kensaku Sakamoto 197102...you are working off of a previously established protocol, make sure to match the growth medium, ncAA concentration... pMAH2-CageCys leucyl-tRNA-synthetase E. coli photocaged cysteine Mammalian TAG Huiwang Ai 73544 pEvol-pAcFRS...91705 pSupAR-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Bacterial TAG P....91706 pCMV-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Mammalian TAG P.... PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser...

  12. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Resources A detailed protocol for practicing OPEN is available in the 2009 Joung Nature Protocols paper . How ... Scot Wolfe Lab plasmids Detailed Information Protocols & Resources Contents of Kit Original Publication...Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder...Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols . 2009;4(10):1471-501. doi: 10.1038/nprot.2009.98...

  13. Antibody Production

    Type
    Collection
    ...For more details on protocols see the Addgene Protocols page or the Neuromab Protocols (Link opens in a ...are initially titered by spectrophotometry using a NanoDrop spectrophotometer. If needed, the sample is...before distribution. Details about our production protocols, titering methods, and quality control are described...Recipient Instructions Addgene Help Center Antibody Protocols...

  14. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...“Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple ...sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. 5, pp. 789–98, Jan. 2009. PubMed ...

  15. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...actually answer your question? If you're using a new protocol or reagents, do you understand all of the steps.... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...

  17. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...from lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain...works in the tissue culture hood while wearing PPE. Photo by Kate Harten DeMaio. Cell lines and reagents...Addgene.org Check out Addgene's written and video protocols ...

  19. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Return to top Photoactivatable Including photoswitchable, photoconvertible, and photoactivatable fluorescent...Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable The lab of Michael Davidson from Florida State...opens in a new window) for images, tutorials, protocols, and more. Empty Backbones Plasmid Description...

  20. Plasmids for Stem Cell Research

    Type
    Collection
    ...activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski Lentivirus Human Expression of human Sox2, Nanog... doi: 10.1038/nature10323. Crabtree Fibroblasts Motor Neurons Retroviral Mouse/Human Conversion of mouse...mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):205-18....2015 Nov 5;17(5):543-56. Buganim Skin Fibroblasts Motor Neurons Lentiviral Human Direct Lineage Reprogramming...Reprogramming Reveals Disease-Specific Phenotypes of Motor Neurons from Human ALS Patients. Cell Rep. 2016 Jan...2023(15)30159-6. Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN Human Transcription Factor-...Differentiation of Human iPSCs into Neurons. Curr Protoc Cell Biol. 2018 Jun;79(1):e51. Next generation ...
Showing: 201 - 220 of 660 results