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Showing: 201 - 220 of 660 results
  1. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post
    ...you’ve gained experience working with similar protocols, and look - they’re seeking someone who has, to...Maybe there was a time you noticed an issue with a protocol and you took it upon yourself to fix the issue...you are in charge of completing many meticulous protocols or tasks, you’ve probably developed an attention...
  2. Summer SciComm Series: Modes of Communication

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    Blog Post
    ...and ecology can make for beautifully compelling photos and videos, while molecular, micro, and cell biology...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...context via still images. Some things cannot be photographed or need text to properly contextualize. Graphics...
  3. Droplet Digital PCR for AAV Quantitation

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    Blog Post
    ...Learn our protocol for AAV titration by qPCR Browse our collection of viral vector related protocols Learn...Browse our blog posts related to viral vector protocols and tips Read all of our viral vectors blog posts...
  4. Antibody Production

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    Collection
    ...For more details on protocols see the Addgene Protocols page or the Neuromab Protocols (Link opens in a ...are initially titered by spectrophotometry using a NanoDrop spectrophotometer. If needed, the sample is...before distribution. Details about our production protocols, titering methods, and quality control are described...Recipient Instructions Addgene Help Center Antibody Protocols...
  5. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...producing vectors from plasmids — including the protocols we use in our lab — being able to order high quality...educational resources, from eBooks to guides to protocols, which was why I was so excited to delve into ...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology, 115, 31.5.1-31.5.13. https...
  6. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can... target sequence has been modified!  The exact protocol may vary depending on your specific experiment...
  7. QC Sequencing Technologies at Addgene

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    Blog Post
    ...them. DNA or RNA is pulled through the pore via motor proteins. When each nucleotide passes through the...Next‐Generation sequencing Technologies. Current Protocols in Molecular Biology, 122(1). https://doi.org/... Molecular Biology Reference Sequence Analyzer Protocol: Sequence Analysis of a Plasmid ...
  8. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...genome with two-step allelic exchange." Nature protocols 10.11 (2015): 1820. PubMed PMID: 26492139. PubMed...bacteria using homologous recombination." Current protocols in molecular biology 106.1 (2014): 1-16. PubMed...molecular biology reference Watch molecular biology protocol videos ...
  9. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    Blog Post
    ...protein characteristics such as the brightness, photostability, maturation time, and acid sensitivity of various...sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated...PMC490022. Shaner, Nathan C., et al. "Improving the photostability of bright monomeric orange and red fluorescent...
  10. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    Blog Post
    ... (Cai et al., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red)..., expect to see systems that use newer, more photostable fluorophores and that can be combined with new...Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson, Alexander...
  11. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...using primers (with the desired mutation) in a PCR protocol that amplifies the entire plasmid template. The...simply following the polymerase manufacturers’ PCR protocol. Be sure to adjust the extension time to match... on Addgene.org Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment...
  12. Using Phosphoserine to Study Protein Phosphorylation

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    Blog Post
    ...PhosTag gels as described above. Reagents and protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial...Addgene # 68307 For further technical reference and protocols for use of this system, please reference ‘Rinehart...Western blotting using Phos-tag containing gel." Nat. Protoc. doi 10 (2008). Journal. Resources on the Addgene...
  13. Michael Davidson Fluorescent Protein Collection

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    Collection
    ...Return to top Photoactivatable Including photoswitchable, photoconvertible, and photoactivatable fluorescent...Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable The lab of Michael Davidson from Florida State...opens in a new window) for images, tutorials, protocols, and more. Empty Backbones Plasmid Description...
  14. Plasmids for Stem Cell Research

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    Collection
    ...activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski Lentivirus Human Expression of human Sox2, Nanog... doi: 10.1038/nature10323. Crabtree Fibroblasts Motor Neurons Retroviral Mouse/Human Conversion of mouse...mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):205-18....2015 Nov 5;17(5):543-56. Buganim Skin Fibroblasts Motor Neurons Lentiviral Human Direct Lineage Reprogramming...Reprogramming Reveals Disease-Specific Phenotypes of Motor Neurons from Human ALS Patients. Cell Rep. 2016 Jan...2023(15)30159-6. Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN Human Transcription Factor-...Differentiation of Human iPSCs into Neurons. Curr Protoc Cell Biol. 2018 Jun;79(1):e51. Next generation ...
  15. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...“Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple ...sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. 5, pp. 789–98, Jan. 2009. PubMed ...
  16. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post
    ...from lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain...works in the tissue culture hood while wearing PPE. Photo by Kate Harten DeMaio. Cell lines and reagents...Addgene.org Check out Addgene's written and video protocols ...
  17. 9 tips for a successful postdoctoral experience

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    Blog Post
    ...actually answer your question? If you're using a new protocol or reagents, do you understand all of the steps.... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...
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