We narrowed to 129 results for: primer

...post, Plasmids 101: Cre-lox, if you need a quick primer on how Cre-lox recombination works. Advances in...

.... For a list of commonly used primers, check out our sequencing primers page . Types of Plasmids Plasmids...enzyme, the template DNA to be copied, and a primer. A primer is a small piece of DNA, approximately 18–...Molecular Cloning Techniques Promoters Sequencing Primers Origins of Molecular Genetics The concept of genes...sort the cells by visualization or flow cytometry. Primer Binding Site A short, single-stranded DNA sequence...amplification or DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert...know some of its sequence to design a effective primer. To learn more about the sequencing technologies...approximately 500–1,000 bases downstream of the known primer region with very few errors, making it an efficient...

...inserts following colony PCR of 10-20 colonies using primers bordering the multiple cloning site of the vector...

...select the appropriate pair of vector and gene primers from Table 2 of the associated publication which...of sequence homology between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and...

...University.     Additional Resources Online Microscopy Primer on the Molecular Expressions Website Other major...

...assay or screen (the Addgene blog has an excellent primer on FRET and how to measure it). Molecular mailbox...

...ICC and PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift...

...into the plasmid backbone, and what sequencing primers can be used to verify it. If your plasmid has multiple...

...vector (the procedure will simply differ in terms of primer design). Let's assume that your favorite vector...

...Complementary overhangs are built into the PCR primers for the insert, based on the destination vector...Additional Resources Addgene Webpages Sequencing Primers Modular Cloning (MoClo) Guide Choosing Your Perfect...