We narrowed to 130 results for: primer

1. Harnessing Bacterial Toxins for Allelic Exchange

   Type

   Blog Post

   ...rate of the organism of interest, from the day primers arrive, the desired chromosomal modification can...

2. Plasmids 101: Biotinylation

   Type

   Blog Post

   ...Polymerase Chain Reaction (PCR) by using a biotinylated primer. Biotin can also be enzymatically incorporated ...

3. Plasmids 101: Restriction Cloning

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   Blog Post

   ...you should send it for sanger sequencing using primers that will allow you to read over the insert. See...

4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   ...post, Plasmids 101: Cre-lox, if you need a quick primer on how Cre-lox recombination works. Advances in...

5. Technique: Probe Phage Genomes for Host Binding Proteins

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   Blog Post

   ...inserts following colony PCR of 10-20 colonies using primers bordering the multiple cloning site of the vector...

6. 22 Hot Plasmid Technologies from 2014

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   Blog Post

   ...select the appropriate pair of vector and gene primers from Table 2 of the associated publication which...of sequence homology between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and...

7. How to Deposit Your Plasmids with Addgene

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   Blog Post

   ...into the plasmid backbone, and what sequencing primers can be used to verify it. If your plasmid has multiple...

8. Which Fluorescence Microscopy Techniques is Best for Me?

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   Blog Post

   ...University.     Additional Resources Online Microscopy Primer on the Molecular Expressions Website Other major...

9. Special Delivery: Fluorophore Targeting for FRET Studies

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   Blog Post

   ...assay or screen (the Addgene blog has an excellent primer on FRET and how to measure it). Molecular mailbox...

10. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post

    ...ICC and PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift...