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We narrowed to 643 results for: ato

Showing: 331 - 360 of 643 results

  1. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...

  2. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency...line and ~8 and 9% efficiencies in T-cells and hematopoietic stem cells respectively. Future researchers ...

  3. March for Science

    Type
    Blog Post
    ...know is your area code. Senators: To find contact information for your senators all you need to do is select...

  4. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...phosphorylation sites. If knowledge of a candidate regulatory kinase is known, it’s possible to generate non-phosphorylated...Often, however, researchers lack knowledge of the regulatory kinase, access to active kinase for the reaction...

  5. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system...W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust ...

  6. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...academic publishing goals and make you a better communicator overall. Don’t Write in a Vacuum Your research...through many iterations read by your PI, your collaborators, others you know in the field, and even people...

  7. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization ...RNA-seq would be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would...

  8. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...

  9. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...

  10. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...

  13. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer vector...

  14. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...absorbed by matter, they promote electrons within atoms to a higher energy state. This process is called.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

  15. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... unbiased discovery. With these screens, the investigator generates a cell population where all genes ...contexts. Most of the immune cells we study are migratory, and their exact localization is important for...

  16. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...and purification is a snap using thin layer chromatography (Fessenden, 2009). However, these reagents ...

  17. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... electrophysiology data too but that’s what collaborators are for). For example, if you study neural pathways...projection-specific neuronal populations using combinatorial viral approaches. Current protocols in neuroscience...

  18. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...any applications. DNA quantity is fairly self explanatory and achieved by performing different sized preps...about!) are a significant source of waste in laboratories. To help combat this, some manufacturers have...

  21. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...essential to build up our current understanding of neuroanatomy and neural connectivity, providing valuable ...conditional transgenic mice, to reveal not only the anatomy of neuronal connections, but to visualize neuron...

  23. Zinc Finger Consortium Reagents

    Type
    Collection
    ...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional...

  24. KLF Research Plasmids

    Type
    Collection
    ...family of at least 17 DNA binding transcriptional regulators with diverse roles in processes such as cell ...

  25. Fluorescent Protein Guide

    Type
    Collection
    ...signals, cells, or groups of cells with either actuators or sensors. Chemogenetics Plasmids | Guide Use...

  26. AAV Viral Preps

    Type
    Collection
    ...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic...

  28. CRISPR Plasmids and Resources

    Type
    Collection
    ...Transcriptional Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9...

  29. Adenovirus Plasmids

    Type
    Collection
    ...double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses...

  30. Retrovirus Plasmids

    Type
    Collection
    ... variant that has WRE, a post-transcriptional regulatory element that increases export to the cytoplasm...
Showing: 331 - 360 of 643 results