We narrowed to 387 results for: nans

1. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   ...on commercial lateral flow strips, similar to pregnancy tests. With lateral flow strips, the presence ...

2. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   ..., Kat Milligan-Myhre, and Pragati Chengappa.  Financial and moral supporters Denise Akob, Sara Alexander...

3. Healthcare Consulting: A Door to the Business of Life Sciences

   Type

   Blog Post

   ...marketing, commercialization, collaborative, and financial functions associated with the business. Commercial...

4. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

   Type

   Blog Post

   ...three-month generation time and labor-intensive maintenance compared to invertebrate model systems, and their...

5. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   ...antibodies in plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-...

6. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   ...suggested there was a single gene that had two codominant versions or alleles that was driving the PHP....

7. Lab to Office Culture Shock

   Type

   Blog Post

   ...also need to talk to Tony from legal, Diana in finance, and Wu in graphic design. You could dread these...

8. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   ...restriction-modification systems mediate plasmid maintenance." Journal of bacteriology 177.12 (1995): 3451...

9. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   ...13. https://www.frontiersin.org/articles/10.3389/fnana.2019.00093 McDade, J. R., Waxmonsky, N. C., Swanson...

10. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    ...Leonard, A. C. (2018). Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome...

11. Hot Plasmids - November 2023

    Type

    Blog Post

    .... (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

12. Viral Production at Addgene

    Type

    Blog Post

    ...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...

13. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    ...could move your project onto the exciting—and unanticipated—road of discovery. Monitoring behavioral output...

14. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    ...28401765. 15. Takai, Akira, et al. "Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging...

15. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    ... cells, HDR is relatively rare and DSBs are predominantly repaired by NHEJ. While other groups have focused...

16. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    ...fragments are likely non-functional, they could be dominant-negative and/or toxic. A potential solution is...

17. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    ...Protein Expression Kit has been used to produced recombinant proteins for in-vitro assays: Koch, Daniel, ...

18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    .... Cell Rep. 2016 Apr 5. PubMed PMID: 27068471. Ramanan V, Shlomai A, Cox DB, Schwartz RE, Michailidis ...

19. Five Popular Model Organisms

    Type

    Blog Post

    ...gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences." The...

20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    ...-3_10 Vergani-Junior, C. A., Tonon-Da-Silva, G., Inan, M. D., & Mori, M. A. (2021). DICER: structure, ...

21. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    ...  More resources on Addgene.org Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide...

22. Antibodies 101: Introduction to Immunofluorescence

    Type

    Blog Post

    ...Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

23. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    ...The size of the intended edit is the biggest determinant when selecting a type of donor. ssDNA templates...

24. Pouring LB Agar Plates

    Type

    Protocol

    ...recommended temperature should kill most potential contaminants, certain types of spores will stubbornly survive...

25. Western Blot

    Type

    Protocol

    ... Antibody Guide Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Introduction Western...

26. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    ...https://doi.org/10.1093/nar/gkw159 Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler...

27. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    ...or when the gene is constitutively-active or a dominant-negative mutant. Reducing the copy number can ...

28. Finding Your Perfect Job After University

    Type

    Blog Post

    ...American system), in 2009 (at the beginning of the financial recession) I knew obtaining work in scientific...

29. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    ...administered systemically, without causing disease? Recombinant adeno-associated viral vectors, or rAAVs, can...

30. CRISPR 101: Off-Target Effects

    Type

    Blog Post

    ...CRISPR work, this often means DNA cleavage at unanticipated sites. While off-target effects are nearly impossible...