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We narrowed to 881 results for: NSI

Showing: 551 - 600 of 881 results

  1. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...production, assembled AAV vector particles accumulate inside the producer cells and purification protocols often...the outside surface of the exosome or are carried inside the vesicle, which may also contain other cellular...

  2. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...new institute and mentor that they may want to consider for future work placements. The chance to learn... expectations are reasonable, your reagents are onsite before you need them, and you are set up for success...

  3. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...model yeast strain and discuss why you may want to consider a yeast off the beaten path.   Yeast as a model...for your media buck - it grows at higher cell densities than most of its counterparts, yielding higher...

  5. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Although highly useful, this technique requires extensive sample processing to avoid disrupting ribosome-mRNA...protein levels were negatively correlated with GFP intensity, showing that TRICK is an accurate readout of ...

  8. Science Career Options

    Type
    Blog Post
    ...computer programming skills? Other things to consider are wage, opportunity to advance, and how many...jobs are normally available (as sectors such as forensics currently have too many applicants compared to...

  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...site of that. 3. Stem cell science is hard, and expensive Dr. Yamanaka explained that it took 50 people ...technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster presenters...

  11. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...fluorescent protein fusions allowed us to image R bodies inside cells, and these constructs may be useful to future... S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive ectosymbionts of Euplotidium (Ciliophora...

  12. Components of CRISPR/Cas9

    Type
    Blog Post
    ...CRISPR/Cas systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

  13. Antibodies 101: ChIP

    Type
    Blog Post
    ...is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works by using...exploring these interactions, and can give valuable insight into DNA expression and protein interactions, including...

  14. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ... effects. However, these methods can be labor intensive and quite time consuming.   As Cas9/gRNA activity...several systems to do this but most are labor-intensive, require multiple fluorescent proteins, and aren...

  18. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...the displaced strand.   Figure 1: EvolvR consists of an error-prone polymerase (PolI) that is fused...provide a better understanding of the key residues responsible for this protein-drug interaction.   The Dueber...

  21. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...Cas enzymes and variants available. Without a comprehensive resource, it can be overwhelming to choose a...resources throughout the site. In the Experimental Considerations section on each enzyme page, you'll find a ...

  22. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...integrated into the genome of the target cell or transiently expressed. It’s important to note that for all...vector can express your construct in target cells transiently or constitutively depending on the virus used...

  25. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...And there are the usual transfection drawbacks: transient expression and needing to work with a transfectable...For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw...

  26. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...monoclonal Cas9 background because you’ll have more consistent levels of Cas9 expression between experiments...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs ...

  27. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...inefficiently that no industrial operation would ever consider them worth the trouble.  Enter ELSA: E. coli Learning...for research.  We’ve also involved ourselves in extensive outreach programs to encourage and embolden the...

  28. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ... and gotten unusual or unexpected results? Or considered why DpnI will degrade your template DNA from ...level, the potential for methylation should be considered when digesting DNA. Why? Well, even though Dam...

  30. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...increase activity and decrease the gRNA size by intensively remodelling the gRNA scaffold through five rounds...tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable of correcting...

  31. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...your network. If someone has connected with me, I consider them fair game for connecting to others.  Pro ...including and especially the hiring manager. This is considered intrusive and it’s still connecting with a stranger...

  32. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Streak on them an E. coli  strain known to be sensitive to that antibiotic, and if the strain is growing...positive clone after these steps, you might want to reconsider your approach, and try a different cloning strategy...

  34. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...phosphorylated MEK1/2 levels, suggesting PP1/PP2A is responsible for this MEK1/2 negative feedback. 3. Studying...Silvia Carrasco, and Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in...

  37. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during cell division....E. coli do not contain the F plasmid (and are considered F-); however, there are a few popular lab strains...

  39. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...abundance before and after a treatment. The library consists of a pool of lentiviral vectors with roughly 73...populations, they found that similar barcodes consistently made up the majority of the resistant populations...

  40. AAV ddPCR Titration

    Type
    Protocol
    ... 10% bleach. Safety Warnings AAV is generally considered biosafety level 1 but may require BSL-2 handling...45° angle. Count to 20 while slowly and gently dispensing the droplets down the side of the tube. Place... 95 10 2 1 Denaturation 95 0.5 2 50 Annealing/Extension 60 1 2 50 Signal Stabilization 98 10 2 1 Hold ... should be close to zero (B08). At Addgene, we consider runs with an NTC > 10 to be invalid. To reduce...

  41. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...scientists to confidently choose reliable and consistent antibodies for specific markers. The Screening...production methods    NeuroMab and others have an extensive library of monoclonal antibodies; Addgene collaborates...

  47. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...Special breeding cases Whether your mouse colony consists of genetically engineered mice with a single mutation...strains that have been crossed, there are many considerations for effectively breeding your mice. Because...

  48. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...abundance of your targets isn’t too different, consider antibody affinity: stronger binding antibodies...membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won’t evenly re-absorb...

  49. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... crossing the blood-brain barrier.  Another consideration when picking ligands for chemogenetic studies...response you aim to manipulate. It is important to consider that the same receptor can elicit different responses...

  50. Engaging with science and society at pgEd

    Type
    Blog Post
    ...institutions. The conversations often move quickly into sensitive topics and uncomfortable places, from sticky ethical...rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull...
Showing: 551 - 600 of 881 results