Addgene

We narrowed to 649 results for: SIM

Showing: 551 - 600 of 649 results

Results per page:

Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

Type

Blog Post

...and CFP can activate certain T-DDOG constructs similarly to GFP, but commonly used red fluorescent proteins...
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

Type

Blog Post

...biotechnology today are things like butanol - very simple molecules - a few carbons and oxygens. Nothing ...
Plasmids 101: Cre-lox

Type

Blog Post

...site-directed recombination technology very conceptually similar to Cre-lox, with flippase (Flp) and the short flippase...
Working with Nuclear Receptors

Type

Blog Post

...website, the new version of which will feature a simplified user interface, expanded numbers of curated molecules...
Design Tips for Prime Editing

Type

Blog Post

...Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt...
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

Type

Blog Post

... of the transcribed ncRNA (Jiang et al. 2014). Simple knock-out experiments are trickier when it comes...
Antibodies 101: Stripping and Reprobing Western Blots

Type

Blog Post

...first round of detection are actually gone. First, simply repeat the imaging step to check if any secondary...
Hot Plasmids February 2024

Type

Blog Post

...mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to the original...
Fluorescent Proteins 101: Introduction to FRET

Type

Blog Post

... permit the use of multiple FRET biosensors simultaneously would further increase the amount of correlated...
Educational Planarian Tools for Teaching Developmental Biology Techniques

Type

Blog Post

...vector backbone. The empty vector PLL4, which is similar to PR-T4P, is available from Addgene. If you use...
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Type

Blog Post

...activity, commonly via light-activated ion channels. Similarly, chemogenetic tools are designed channels that...
Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

...system from Jiazhang Lian’s lab that enables simultaneous gene activation, repression, and editing with...
A Guide to Designing a Scientific Poster: Content, Layout, and More

Type

Blog Post

...these colors so that your text is easily read. Similarly, be cognizant of audience members that are color-blind...
Engaging with science and society at pgEd

Type

Blog Post

...and how you can set yourself on a path towards a similar position. How pgEd broadens the conversation about...
Adeno Associated Virus (AAV) for Cell and Gene Therapy

Type

Blog Post

...based delivery techniques may hold the key. In the simplest form, translating gene therapies from conceptual...
5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

...your virus experiments. References 1. Anthony, Simon J., et al. "A strategy to estimate unknown viral...
Tips for Titering Your Lentiviral Preps

Type

Blog Post

... parallel, this approach should be considered. Similar to the p24 assay, titering via measurement of viral...
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Type

Blog Post

...long hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they ...
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

Type

Blog Post

...after receiving her degree she was faced with a similar conundrum – she had the skills, training, and motivation...
Production of Virus in Insect Versus Mammalian Cells

Type

Blog Post

...Processing of your baculovirus is straightforward — simply spin down the supernatant in a centrifuge to remove...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

... of different pegRNAs for each desired edit, it simply becomes impossible (or at least unreasonable) to...
Pooled Library Amplifications

Type

Blog Post

...looking at protocols used for libraries that are similar in size and composition as examples. Table 1 provides...
Twenty Years of Addgene Sharing: CRISPR

Type

Blog Post

... and requesting each year (Figure 2). We did a similar analysis with CRISPR pooled libraries, although...
Plasmids 101: Degron Tags

Type

Blog Post

...which target E3 ligases to specific proteins by simultaneously binding both their target protein and an E3...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Type

Blog Post

...examine your images using subjective judgement by simply “looking at them”. Modern cameras and image analysis...
Fluorescent Proteins 101: Fluorescent Protein Timers

Type

Blog Post

...protein or protein fusion. This one protein method simplifies cloning and makes your experimental setup less...
10 Basic tips for mammalian cell culture

Type

Blog Post

...identified. Even if some mammalian cells lines have similar morphologies, being familiar with these morphologies...
xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7...
Of Myc and Men

Type

Blog Post

...for the foreseeable future. These newer findings simply add to our understanding of how this antibody works...
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Type

Blog Post

... that can bind effectively to protein targets, similar to how antibodies do. The structure of the common...
The Scientific Conference Poster Session: Tips for Success

Type

Blog Post

...difficulty answering questions. Or perhaps you’re simply attending, but uncertain how to capitalize on your...
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Type

Blog Post

... design to analyzing results. To use this tool, simply follow these steps: Create a new experiment in...
Google Forums Round Up: First Impressions of NgAgo

Type

Blog Post

...inexpensive and can be 5’-phosphorylated in house using a simple PNK reaction or can be purchased 5’-modified from...
Plasmids 101: Optimizing Plasmid Yields

Type

Blog Post

...yields. Why would volume make a difference? In the simplest of terms, more volume = more cells = more plasmid...
Experimenting with New Careers while in Grad School

Type

Blog Post

...researchers. It is an excellent way to meet people with similar interests! Especially as someone interested in ...
Anatomy of a Plasmid Page at Addgene

Type

Blog Post

...depositing lab. There may be other plasmids with similar names in the collection, but you can use this name...
Light Sheet Fluorescence Microscopy

Type

Blog Post

...transparent organisms. Advances in tissue clearing Similar to light microscopy, there have been significant...
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Type

Blog Post

...organisms with Cas9 risk creating a gene drive? The simplest way to make a gene drive is to insert an endonuclease...
Antibodies 101: Producing Recombinant Antibodies

Type

Blog Post

... own proteins, making the purification process simpler. Due to its high yields, E. coli is often used ...
Plasmids 101: Choosing an Antibiotic Resistance Gene

Type

Blog Post

...synthesis as their mode of action and have a very similar relationship as amp and carb (they are interchangeable...
CRISPR 101: Making a Knock-In Cell Line

Type

Blog Post

...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...
Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Type

Blog Post

...mentioned above, organic solvents can be used to simultaneously fix and permeabilize a sample. But you can ...
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Type

Blog Post

...between Jan. 1 and Mar. 1, 2020. Figure 2: A simple approximation of the effect of COVID-19 on data...
Isolating Genomic Regions of Interest with the CRISPR System

Type

Blog Post

...and RNA sequencing (Fujita et al., 2015). Using similar affinity purification with an anti-Cas9 antibody...
Droplet Digital PCR for AAV Quantitation

Type

Blog Post

...measure the concentration of multiple targets simultaneously. For AAV titration, you should only need to...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

...RNPs are capable of cleaving genomic targets with similar efficiency as compared to plasmid-based expression...
Summer SciComm Series: Modes of Communication

Type

Blog Post

...or expertise from the start, or if you can begin simply and build up to more complexity if needed. And ...
Better Dyeing Through Chemistry & Small Molecule Fluorophores

Type

Blog Post

... from fluorescent proteins given the relative simplicity of the system and the availability of fluorescent...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

... 10 meters apart, are mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly...

Showing: 551 - 600 of 649 results

Results per page: