We narrowed to 293 results for: CRISPR-Cas9

... Addgene Blog CRISPR libraries Genome-wide screens with CRISPR Lentiviral CRISPR libraries ....S. Genetic screens in human cells using the CRISPR/Cas9 system. Science, 343(6166): 80-84 (2014). doi...with small molecules/drugs. With the advent of CRISPR, genome-wide knockout screens are possible with...While the entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically...downstream manipulations as a forward or reverse screen (CRISPR, drug, etc.), just with an added baseline component...component. Alternative screening techniques While CRISPR and drugs dominate the screening scene, there are... worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes of interest...

...plant, Dr. Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants that are able to...the anti-phage CRISPR effect observed in bacteria. Learn more about the different CRISPR applications in...Resources at Addgene.org Check out our CRISPR resources and CRISPR for plants Bacterial Expression Systems...

... depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...Gene Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...

...RNA-guided genome editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon system with the...site-specific target-primed insertion through targeted CRISPR homing of retroelements." The Abudayyeh-Gootenberg... Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened R2Tocc transposon protein, ...for scarless insertion of the payload (teal). The Cas9 gRNA targeting guide and the transposon RNA homology...scientists fused the retrotransposon protein R2Tocc to a Cas9 nickase to improve target recruitment and nicking...TurboCas provides the genomic targeting power of dCas9 with the dynamic labeling power of miniTurboID, ...proximity ligation tool (Cenik et al., 2024). While dCas9 has been used in combination with proximity labeling...

.... Currently, AAVs are being used to deliver CRISPR/Cas9 but have the potential to deliver other therapeutic...used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient...Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional Resources...

...mismatches within a CRISPR guide can prevent the guide from binding to the genome or prevent Cas9 from cutting...extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking to uncover... and Boehm labs pulled data from more than 1000 CRISPR screens in DepMap to see if they could identify...Since about 90% of the cell lines used in DepMap CRISPR screens are of either European or East Asian ancestry...it was coming through an unexpected source: the CRISPR guides, short pieces of RNA that bind to a cell...does the reference genome actually reference?  The CRISPR guides used in the screens they analyzed were designed...map to thetargeting sequences of guides across 8 CRISPR libraries. Samples are divided into those of African...

... out our CRISPR Featured Topic Page Learn about RNA Targeting with Cas13a Learn about CRISPR DNA Base ...Resources on Addgene.org Browse All CRISPR Plasmids Check out our CRISPR Guide Pages ...enzymes are quickly becoming major players in the CRISPR field. Just a year after Feng Zhang’s lab identified...C2c2) (Abudayyeh et al., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for precise RNA editing...programmable A to I (G) replacement) is the first CRISPR tool for RNA editing. Two years after that, the...the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes prefer targets with a given... targeting work with Cas13, REPAIR is the first CRISPR-based system to enable precise RNA editing. The...

...organism.   It’s not an overstatement to say that CRISPR/Cas9 editing is one of the key tools now providing...clutch of eggs? Propagation is thus quite difficult. CRISPR genome editing in squid is possible nonetheless...reptiles at all. But new success is coming with CRISPR/Cas in lizards. The Menke Lab was able to make ...-Mediated Ovary Transduction of Cargo)  delivers Cas9 RNP to the arthropod germline by injection into ...

...Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus...Staphylococcus aureus (SaCas9). 6, 8 Feng Zhang Labeling Tools These AAV encode tools that can be used...

...cells By Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells ...peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing...lines and primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due ...multiplexed guides. Figure 1: A) Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization...Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter...

...Protocol (DOCX, 17 KB)   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional ...Amplification Type Link to Protocol CRISPR Knockout Human CRISPR Knockout Pooled Library (Brunello) ...Amplification Protocol (PDF, 88.2 KB) CRISPR Knockout Human CRISPR Knockout Pooled Library (GeCKO v2) ... Protocol (DOCX, 15 KB) CRISPR Activation Human Genome-wide CRISPRa-v2 Libraries 104,540–209,080...Re-Amplification Protocol (PDF, 71 KB) CRISPR Activation Human Subpooled CRISPRa-v2 Libraries 12,285–20,145 ...ng Solid Joung et al. Nat Protoc. 2017 CRISPR Knockout Human Epigenetic Knockout Library ...on the Addgene blog Genome-Wide Screening Using CRISPR Preparing Pooled Libraries NGS Quality Control ...

...fluorescent ligands to HaloTag PCV-Cas9, Cas9-PCV HUH-tag Cas9 plasmids with N- or C-terminal PCV2 HUH-tag... CRISPaint Gene Tagging Kit SpyTag/SpyCatcher CRISPR-assisted insertion tagging Kit; integration of ...

...Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation...Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson...

... Plasmid Collection You May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide... R. N. (2017). Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization...

...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out...Lentiviral vectors are the vector of choice for many CRISPR applications, and they’ve also had success in clinical...transgene-expressing or knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including... Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods...Lentiviral Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening...including Addgene’s top requested plasmid in 2015: lentiCRISPR v2, used both for targeted modification and genome-wide...

...efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system...in comparison to the 62-90% error rate seen with Cas9 editors. The lab believes that this system could...inactive dCas9 and microbial SSAPs (dCas9-SSAP) which insert genes in a cleavage free manner. The dCas9-SSAP...cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang, et al, 2022.  Find dCas9-SSAP...Cre-mediated AAV Targeted Evolution method (M-CREATE) dCas9-SSAP with reduced error rate for long sequence knock-ins... https://doi.org/10.1038/s41593-021-00969-4.   dCas9-SSAP with reduced error rate for long sequence knock-ins...Wilson Le Cong’s lab has developed a method using dCas9-SSAP to knock-in long sequences with reduced risks...

...our journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept...Blog Experimenting in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science...

...48-well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western ...Addgene Blog Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture...; more recently we have also been using Addgene CRISPR vectors to generate cell lines with knockouts of...

...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab...

...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening... engineering. Read more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase the risk...such as Cas9 or Cas12a. These are identified in their names — AcrII proteins inhibit Cas9 (CRISPR Type II... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...Figure 7: Overview of CRISPR transposases. Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases... using CRISPR libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...genomic regions using CRISPR. Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S...