We narrowed to 38 results for: CRISPR-Cas9

... cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips...sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your...ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel...

...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening... engineering. Read more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase the risk...such as Cas9 or Cas12a. These are identified in their names — AcrII proteins inhibit Cas9 (CRISPR Type II... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...Figure 7: Overview of CRISPR transposases. Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases... using CRISPR libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...genomic regions using CRISPR. Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S...

...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors ...of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9 is a versatile genome editing technology...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system can be...sequence (20bp). CRISPR-Cas9 Cre expression vectors for genome editing in the brain Using Cas9 mice Platt et... AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing and cancer...Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR... 24. PubMed . Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9 knockout screening in human...

...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very ...finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced...prokaryotes used CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas...

...engineering, including CRISPR/Cas9 parts. CRISPR Diego Orzaez 1000000159 MoClo CRISPR/Cas Toolkit for Plants...membrane and nuclear markers. CRISPR Alexis Maizel 1000000151 CRISPR-Cas9 Gateway System for Physcomitrella...Transformations Engineering the Plant Genome Using CRISPR/Cas9 CRISPR-mediated Plant Base Editors RNA Interference...for assembly of CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in ...cloning and multigene engineering, including CRISPR/Cas9 parts. Golden Gate (GoldenBraid) Diego Orzaez...Cloning System (#1000000036). Includes modules for CRISPR/Cas9 genome editing, dexamethasone-inducible expression...sequences, basic CRISPR parts, and parts for modular geminiviral replicon assembly. CRISPR Daniel Voytas ...

...elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that... individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's... tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's...feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic...Addgene's worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs ...Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Mos1 Based Transgenesis...Addgene Blog Posts An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ...from the Xing Chang lab and CRISPR-X from the Michael Bassik lab , in which Cas9 is fused to activation-induced...1: Overview of CRISPR base editing. Browse, sort, or search the tables below for CRISPR base editing plasmids...learn more about base editing and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...

... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan ...information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus on... on making single edits using CRISPR/Cas9 in mammalian cells as an example, but many of these principles...). You will decide how to express Cas9, the delivery system for Cas9 and the guide RNA (gRNA), the design...non-homologous end joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity...insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires...

...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins...information on CRISPR plasmids and function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's...Packaging Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes...Discontinued) Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set A Human CRISPR inhibition library...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Prime Edit CRISPR Plasmids: Prime Edit Mammalian Bacteria...below for CRISPR prime editing plamisds. To learn more about prime editing and other CRISPR topics, read...contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection...Ervin Welker Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme is capable of installing...

...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector ...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...the laboratory use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart... or the zebrafish CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Protein Tagging CRISPR Plasmids...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag...for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system...system consists of two components: An all-in-one CRISPR/Cas9 vector based on the Zhang lab's PX330 , simultaneously...pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for building CRISPR targeting...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...off-target effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR dCas9-FokI ... A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

...for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids...Collections Brzezinski Lab CRISPR Collection Brzezinski Lab CRISPR Collection The Brzezinski lab investigates...involved in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several... plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion...175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR for activation...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...below for CRISPR Cas3 plasmids. To learn more about Cas3 and other CRISPR topics, read our CRISPR Guide .... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... Plant Resources Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... To learn more about CRISPR interference and other CRISPR topics, read our CRISPR Guide . Mammalian ID... CRISPR Interfere CRISPR Plasmids: Interfere...1: Overview of CRISPR interference. Browse, sort, or search the tables below for CRISPR interference plasmids...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Plant Yeast Resources Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...epigenetic modification with CRISPR and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Mammalian Plant ... using CRISPR. Created with BioRender.com. Browse, sort, or search the tables below for CRISPR plasmids...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...modifications, researchers have fused catalytically dead Cas9 (dCas9) to epigenetic modifiers. Common modifiers include...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Parasites CRISPR Plasmids...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and ... Addgene's parasite CRISPR plasmid collection also includes the Toxoplasma CRISPR Knockout Pooled Library...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for...

... of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Lentiviral Preps CRISPR Protocols...MoClo CRISPR/Cas Toolkit for Plants CRISPR Resources Addgene has a large selection of other CRISPR plasmids... CRISPR Empty gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors...multiplex-compatible gRNA plasmids, including: Multiplex CRISPR/Cas9 Assembly Systems Multiple Lentiviral Expression...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Vectors gRNA and Cas gRNA Only Multiplex Resources CRISPR requires you to express both a Cas protein and ...gRNA-only plasmids are not limited to a specific CRISPR function, but require an additional plasmid containing...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...plasmids. To learn more about CRISPR nickases and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid... CRISPR Nick CRISPR Plasmids: Nick...Overview of Cas9 nickase cleavage and repair. Browse, sort, or search the tables below for CRISPR nickase ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...