We narrowed to 469 results for: LARGE
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TypeBlog Post...pieces developed over the years were generated by large agricultural companies and carry associated intellectual...
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Plasmids 101: Mammalian Vectors
TypeBlog Post...virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (293E or 293T cells), allow for episomal... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...diversity of CRISPR systems, only time will tell how large the genome and transcriptome engineering toolbox... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...interact or oligomerize in cells? This question is largely unanswered. Ideally, fluorescent proteins should... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...degree of both specificity and sensitivity across a large number of loci and gRNA lengths. Recent alternatives... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post...relationship-building and influence. You’ll need a large deal of influence and strong relationship-building... -
Hot Plasmids - May 2022
TypeBlog Post...comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system has a much lower error... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, unlike... -
Viral Vectors 101: Viral Vector Elements
TypeBlog Post.... Viral packaging and entry Adenovirus is quite large and has more helper plasmid components than the ... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...trickiness of using and optimizing GCE, led to a large toolbox filled with systems and ncAAs that were,... -
Viral Vectors 101: Viral Applications
TypeBlog Post...viability. Library delivery Screening libraries, large collections of biological independent variables,... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...technical support team, is a safer bet than one from a large distributor that may not have direct experience ... -
Hot Plasmids - February 2022
TypeBlog Post...genome regulation and editing by: Rachel Leeson The large size of CRISPR-Cas effectors and fusion proteins...single expression cassette is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a ... -
Antibodies 101: Secondary Antibodies
TypeBlog Post...This Fc region (the stem of an antibody’s Y) is large enough to allow multiple secondary antibodies to... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...composition available. If you’re going to need a large quantity of antibody for your experiment, you should... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...themselves using computational approaches to analyze large data sets (myself included) and we are faced with... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...re happy to provide them, that never works on a large scale. The world is too big now. Mendenhall: So ... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post... CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens ... -
Protocol - How to Streak a Plate
TypeProtocol...30 °C instead of 37 °C. This is often true for large unstable plasmids, which sometimes recombine at ... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...with the Cas9/sgRNAs vector (like PX330). For large changes (>100 bp insertions or deletions), we typically...lab typically use PAGE purified long oligos. For large changes (>100 bp insertions or deletions), the Zhang...