We narrowed to 464 results for: LARGE

1. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

   Type

   Blog Post

   ...its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, unlike...

2. Viral Vectors 101: Viral Vector Elements

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   Blog Post

   .... Viral packaging and entry Adenovirus is quite large and has more helper plasmid components than the ...

3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   ...interact or oligomerize in cells? This question is largely unanswered. Ideally, fluorescent proteins should...

4. GCE4All: Making Genetic Code Expansion Accessible

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   Blog Post

   ...trickiness of using and optimizing GCE, led to a large toolbox filled with systems and ncAAs that were,...

5. Viral Vectors 101: Viral Applications

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   Blog Post

   ...viability. Library delivery Screening libraries, large collections of biological independent variables,...

6. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   Blog Post

   ...diversity of CRISPR systems, only time will tell how large the genome and transcriptome engineering toolbox...

7. A Quick Guide to a Career in Software Product Management

   Type

   Blog Post

   ...relationship-building and influence. You’ll need a large deal of influence and strong relationship-building...

8. Hot Plasmids - May 2022

   Type

   Blog Post

   ...comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system has a much lower error...

9. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   ...technical support team, is a safer bet than one from a large distributor that may not have direct experience ...

10. Hot Plasmids - February 2022

    Type

    Blog Post

    ...genome regulation and editing by: Rachel Leeson The large size of CRISPR-Cas effectors and fusion proteins...single expression cassette is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a ...

11. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    ...This Fc region (the stem of an antibody’s Y) is large enough to allow multiple secondary antibodies to...

12. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    ...degree of both specificity and sensitivity across a large number of loci and gRNA lengths. Recent alternatives...

13. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    ...composition available. If you’re going to need a large quantity of antibody for your experiment, you should...

14. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    ...themselves using computational approaches to analyze large data sets (myself included) and we are faced with...

15. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    ...re happy to provide them, that never works on a large scale. The world is too big now. Mendenhall: So ...

16. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    ... CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens        ...

17. Protocol - How to Streak a Plate

    Type

    Protocol

    ...30 °C instead of 37 °C. This is often true for large unstable plasmids, which sometimes recombine at ...

18. Antibodies 101: Fab Fragments

    Type

    Blog Post

    ..., two full antibodies bound together may be too large. Using a complex that is one full antibody bound...and have lower binding affinity than some of the larger fragments.  Think of antibody fragments as new ...

19. Human Germline Editing Using CRISPR

    Type

    Blog Post

    ...germline editing. Although this paper represents a large step forward in the CRISPR field, it’s important...

20. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    ... can be excited many times, thereby producing a large number of emitted photons before they are destroyed...