...Detection of Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki... B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before/after application...dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons). Figure and caption adapted from Kagiampaki et...

...our lab’s environmental impact, but we can all do more to make research more environmentally friendly.... to address, but is often ignored in the lab environment. I recently attended the Go green! Innovative...institute! What should I do in the lab to help the environment? Well, you could: Collect the NEB and/or Millipore...

...potential as a scientist. But I expected something wrong. My capstone project was based on plasmid cloning...successful clones because the bands were all in the wrong place. Except for one. My manager speculated that...my very own research project! Coming from an environment with limited opportunities and support, puts ...puts into perspective just how much one’s environment changes them. Addgene gave me the chance to become ...

...transcript You can also multiplex gRNAs via a polycistronic transcript. Rather than being transcribed from...option described below, plasmids for making polycistronic gRNAs are also available from the Yang lab for...Plasmids: These plasmids are similar to the Csy4 polycistronic system described above, except that they use...gRNAs are flanked by glycine tRNAs to create polycistronic glycine tRNA-gRNA (PTG) constructs. Eukaryotic... Find plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized...

...refresher if you ever lose track of which acronym is which. Acronym Full name Notes CRISPR terms gRNA... of CBEs and ABEs. This post includes a lot of acronyms, so we’ve included a glossary at the end if you...activity, and they are sometimes processed by error-prone non-homologous end joining (NHEJ). To mitigate this...edit. NHEJ Non-Homologous End Joining An error-prone cellular process to repair DSBs. Base Editing ...

...Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389...different levels of expression. For example, CAG is a strong promoter that drives very high, ubiquitous expression...expression, while the human synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer...

... paper in Frontiers in Neuroanatomy and summarized some considerations for precise neuronal targeting ...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources...

...available with one sgRNA per operon or gene. The sgRNA pool can also target core operons in other pneumococcal...group optimized their initial system by using a stronger promoter and different biochemical secondary energy...AAV tools to selectively control parvalbumin interneurons from the Dimidschstein Lab More GCaMP8 ready-to-use...

...leaves patients incredibly sensitive to sunlight and prone to developing metastatic melanomas and other cancers...,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012, researchers...sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they now think that...that mutations in the gene may prevent neurons from returning to basal conditions normally after periods...

...photos of our pets. #SocialDistancing may send a wrong message for some people. We should recommend #PhysicalDistancing...the COVID-19 pandemic, check out Care For Your Coronavirus Anxiety and Taking Care of Your Behavioral Health...home, the STEM career exploration site Inventing Heron has a radio reporter challenge, and code.org has...necessarily to chat, but to mimic your usual work environment.  Be kind to yourself. This is a challenging ...

... chow, a different water filtration system, environmental stability in an incubator), but there’s a lot...for a transparent and collaborative research environment. Journals as enforcers Currently, most of the... formatting can feel like yet another hurdle in front of publication when all they really want to do is... of incentives. Right now, researchers are not strongly rewarded for being transparent in their communication...

...improved detection for tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See ...Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also ...chemogenetic AAV for activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab...with Cre for intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV...

...presented chronologically. With a resume however, most often people choose a reversed chronological style ...you did. Emphasize the skills. If you studied astronomy or bioinformatics data, then the chance is that...Communications Coordinator for the Department of Physics & Astronomy, University of British Columbia. Originally on...

...The calcium is strong here….I can sense it….sound like a plot to an biology scifi movie? Or maybe it’...contraction, and calcium channels are activated for neuronal action potentials; it’s an ion of interest for...monitor calcium specifically in their favorite neurons. This specificity is feasible partially because...Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct Vol. 13; 1662...

...generation lentiviral packaging plasmid from the Didier Trono lab that expresses the HIV gag, pol, rev, and tet...pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing plasmid...generation lentiviral packaging plasmid from the Trono lab contains the rev gene. It’s meant to be used...generation lentiviral packaging plasmid from the Trono lab that contains the gag and pol genes. It also...

...flexibility for in vivo proximity labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution...flexibility for in vivo proximity labeling   By Ashley Waldron Proximity-dependent labeling has become a powerful...doi.org/10.1038/s41593-021-00969-4.   Increased neuronal expression using Cre-mediated AAV Targeted Evolution...found at least two variants that display increased neuronal expression in the brain while having decreased...

...beginning. The well-characterized bacterial lac operon contains a gene called lacZ that encodes for the...the enzyme β-galactosidase. Expression of the lac operon is induced by lactose, and also by a lactose analogue...completely accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac expression). When...in the medium to ensure transcription of the lac operon.   Tips for blue-white screening Use a good control...

...separated by 28 base Csy4 recognition sites from a polycistronic transcript. Although this method shortens construct...requires coexpression of Csy4 with Cas9 and the polycistronic transcript. As nucleases may have off-target... of gRNA cleavage.   Xie et. al constructed polycistronic glycine tRNA-gRNA genes (PTGs) in the forms ...Bacteria and viruses have illustrated the power of polycistronic gene organization, and its translation to CRISPR...

...the appropriate ligand - estradiol for hER or progesterone for hPR - leads to nuclear localization, binding...controlled transcription factor and a ZIF268/progesterone controlled transcription factor as well as reporters...with various concentrations of estradiol and progesterone and showed that reporter expression is dependent...cognate inducer. Since induction via estradiol and progesterone do not affect each other, these systems can ...

...autophagy. Morphological assays Electron microscopy: Electron microscopy is a traditional method for...at various stages of degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged...will fluoresce green unless it’s in an acidic environment like the vacuole or lysosome. See Table 2 for...