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We narrowed to 450 results for: abo.5

Showing: 221 - 240 of 450 results

  1. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...conditions described above, and a short description of how the antibody performed.  Figure 5: Results for the...

  3. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post
    ... real-time PCR. Nat Methods. 2013 Oct;10(10):1003-5. PubMed PMID: 22035192. PubMed Central PMCID: PMC3216358...PMID: 20486768. PubMed Central PMCID: PMC2957240. 5.Taylor, Sean C., Genevieve Laperriere, and Hugo Germain... Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols ...

  5. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...rather than the ZymoBrothTM, so it costs us less than 5 cents per transformation. This protocol has worked...own competent cells using the protocols provided above, then you can find the subsequent transformation...

  7. Five Popular Model Organisms, Part 2

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    Blog Post
    ... Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org/10.1159/000490898 Gibbs.... J. (2009). Rats! Disease Models & Mechanisms, 2(5–6), 206–210. https://doi.org/10.1242/dmm.002733 Idalia... more about model organisms in the first Five Popular Model Organisms blog post Read more about plasmids...characteristics that make them ideal for studying in a laboratory environment. We’ve covered five model organisms...have been specifically developed to thrive in laboratory settings. One of its most attractive features...minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive...address many areas of microbial research like metabolism. Transport, biofilms, and much more. Because ...

  8. March for Science

    Type
    Blog Post
    ...consumption in comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe...activity while much less of the general public agrees (5, 6). Many of the new administration’s actions, including... with a testable hypothesis - an educated guess about how some part of the world functions. Experiments...governments exist in reality and science is how we learn about reality. Formally, a government can be defined ... More concretely, scientists gather information about reality informing people and their governments on...people to rally for science, others are concerned about possible unintended consequences of a march. Three...Democrats more often than Republicans, the March is not about Democrats or Republicans (16). This is a Pro-Science...

  9. Plasmids 101: Common Lab E. coli Strains

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    Blog Post
    ...rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- JM109   General cloning...hsdS20(rB-, mB-) ara-14 galK2 lacY1 proA2 rpsL20 xyl-5 leu mtl-1 Stbl4   Electrocompetent cells for cloning...thiamine metabolism Requires exogenous thiamine for growth ara disruption of arabinose metabolism pathway...little prokaryotic minions that carried out the labor-intensive process of replicating your new plasmid... Phage resistance gal Mutation in galactose metabolism pathway Cells cannot grow on galactose only ...repressed more   LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose...plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced recombination...

  10. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ...Regenerative Medicine accelerates research. Chapter 5: Research currently under way in the Kotton Lab. One...promote scientific sharing. Listen to learn more about Darrell, his research, and his focus on open science...Medicine and in the Department of Pathology and Laboratory Medicine. He attends in the Medical Intensive...NIH-funded Principle Investigator in the Kotton Laboratory and is the founding Director of the Center for...Podcast.     Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids...Plasmids from the Kotton Lab Learn more about Stem Cells ...

  11. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...positive vs. negative signal remains the same (Figure 5).   A.                                                                                                        ...                                                                                                        B.      Figure 5: (A) A gate to select the negative live population...lymphocytes in a mouse tumor sample You made it! So much about the theoretical background. Let’s dive into the ...often choose a looser gate here. I am not worried about including irrelevant events in my analysis, as these...population, acquired from the previous gating pipeline, about 53% of my cells are dendritic cells and ~13% are...ve covered in this blog post. First, you learned about the types of scattering that occur when a cell is...

  12. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...promising starting point for further engineering. Figure 5: Structure-guided design and genome mining both led...Genome Mining Strategies. ACS Sens. 2022 May 27;7(5):1336-1346. doi: 10.1021/acssensors.1c02201. LiLac...screen (Koveal et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable...valuable tool to study cellular physiology and metabolism under various conditions. LiLac has improved response...going viral! In addition to the viral preps noted above for iGluSnFR3 and JEDI-2P, several biosensors already...innovation next year! Did we miss any? Let us know about your favorite new biosensor plasmids in the comments...

  13. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...deleting residues. Only about 10 amino acids can be deleted from the C-terminus and about 5 from the N-terminus... 153.7 (2013): 1602-1611. PubMed PMID: 23768684.  5. Thorn, Kurt. "Genetically encoded fluorescent tags...cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel...based on cofactor binding proteins as described above. The smallest tags are peptides that are engineered...

  14. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...J Biol Chem 279.e5 (2004). PubMed PMID: 20353954. 5. Kay, B., Thai, S., & Volgina, V. “High-Throughput...cells and transgenic mice.” PNAS 100.13 (2003):7480-5. PubMed PMID:12802011. PubMed Central PMCID: PMC164612...uses of biotinylation in molecular biology! Learn about in vivo biotinylation of bacterial fusion proteins...covalently attached to the active site of key metabolic enzymes including biotin carboxylase and decarboxylases... organic acids to produce important cellular metabolites. Biotin acts as a carboxyl carrier from one compound...such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction. Biotinylation is the...

  15. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post
    ...Methods. 2013 May 5:10(6):540-7. doi: 10.1038/nmeth.2450. Epub 2013 May 5. PubMed. Zebrabow: multispectral...integration of multiple copies of the construct (about 8 in Brainbow-1.0 mice.) The combinatorial effect...with the goal of overcoming the limitations listed above. First, they screened a variety of fluorescent proteins...method has become very useful in lineage tracing; “Zebrabow” was used to trace the development of the corneal...

  16. Addgene's Top Blog Posts from 2020

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    Blog Post
    ... but what about the other methods to quantify DNA? Here we cover five methods to do this. 5. Plasmids... highlighted reagent repositories and other collaborative efforts in the scientific community to speed...store my plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post....Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids in one cell, it might ...

  17. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...79987/ 642 670 87,400 14.0 198 Monomer  155 4.5 72 5 miRFP703 https://www.addgene.org/79988/ 674 703...for neuronal and tumour brain imaging. Nat Commun 5, 3626 (2014). Shcherbakova, D.M. & Verkhusha, V.V....in eukaryotic cells as an intermediate of heme metabolism (Shemetov et al, 2017). Modern state-of-the-art...reporters and biosensors NIR reporters can tell us about cellular events via  fluorescence changes occurring...NIR-GECOs were developed based on mIFP6. Learn more about the characteristics, performance, and applications...

  18. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...the desired chromosomal modification can be made in 5 days. Counter-selection with sacB has revolutionized... editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason, Lynn... a sucrase that converts sucrose into a toxic metabolite. Cells that have undergone a double crossover...confirmation of successful single crossover – after about 48 hours, the resulting colonies turn subtly blue...blog posts Find our CRISPR blog posts Learn more about Lambda Red recombineering Resources on Addgene....

  19. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...society and patients, and respect industry concerns.” 5)      In the aftermath of the Supreme Court’s decision...study probably doesn’t say as much about open access as it does about the state of peer review. 8)     ...University Open Data Access (YODA) Project, “a collaborative endeavor guided by aspirations to promote open...

  20. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...by in vivo functional screen." Scientific reports 5 (2015). BIOFAB plasmid set 3000 Series Confronted...Translational Sequence (SITS). This sequence bypasses the 5′ mRNA cap required for eukaryotic systems and instead...PUF Assembly Kit Below you’ll find information about what these kits contain, how they can be used, and...encoded sensors enable real-time observation of metabolite production." Proceedings of the National Academy...
Showing: 221 - 240 of 450 results