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We narrowed to 276 results for: guide rna expression plasmid

Showing: 221 - 240 of 276 results

  1. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post
    ... in a plasmid with loxP sites such that their expression is turned on or off in cells expressing Cre. ...Addgene.org Check out Our Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent...allows the visualization of fluorescent proteins expressed in the specimen.  2. Fluorescence Activated Cell...immune lineage, scientists could easily track GFP expressing cells. For example, by harvesting lymph nodes...efforts have been made to create transgenic mice expressing fluorescent protein reporter constructs in the...above depicts the infiltration of neutrophils expressing LysM-GFP (green) into a draining lymph node after...Introduced to immunology by three papers in the journal Science in 2002. Now, this technique and the in...

  2. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ... Hub AAV Guide AAV Packaged On Request Browse all in-stock AAV preps Browse all AAV plasmids ...viral capsid, but specificity doesn’t end there: expression from rAAVs also depends on your choice of promoter..., et al., 2006). AAV2 capsids are available as plasmids in the repository and can be selected as a serotype...efficiency in multiple tissues, rapid transgene expression, and can cross blood vessel barriers. It is especially..., et al., 2006). AAV8 capsids are available as plasmids in the repository, as part of our in-stock viral...retinal cells (Issa et al., 2023). AAV9 packaging plasmids are available at Addgene. AAV9 vectors are not...the axon to the cell body and driving transgene expression (Tervo et al., 2016). This functionality makes...

  3. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post
    ...Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent Protein Guide: Biosensors Keima, another...monitor autophagy, the Pho8Δ60 mutant protein is expressed. Pho8Δ60 localizes to the cytosol in an inactive...yeast Rosella is targeted to the cytosol when expressed without a signal sequence and to the mitochondria...measuring the amount of radioactivity in the culture supernatant (this is indicative of protein degradation). ...

  4. CRISPR 101: Drug discovery and CRISPR

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    Blog Post
    ...Additional resources on Addgene.org CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development...generation of multiple edits or implementation of expression systems to accurately mimic a disease phenotype...best served by this type of targeted design. Alternatively, a disease model can be generated, and drugs...

  5. Fluorescent Protein Guide: Biosensors

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    ...includes 35 plasmids expressing a variety of fluorescent sensors to monitor cell physiology. RNA-based aptamer... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...S-adenosyl methionine . Reporters of miRNAs Toehold sensors for various target RNAs Explore the Fluorescent Biosensor...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 ... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...

  7. The Challenges of Cell Culture

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    Blog Post
    ...Resources ATCC Animal Cell Culture Guide Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen...Invitrogen Cell Culture Basics Guide Abcam Cell Culture Guidelines Resources on the Addgene Blog Learn ...along with Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal...Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...identification of glioma-initiating cells. The International Cell Line Authentication Committee includes ...cell culture outlined in this article. Now many journals strictly require evidence of cell line provenance...

  8. CRISPR-mediated Plant Base Editors

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    Blog Post
    ...the single guide RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven expression system. We ...all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for plants ...We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables ...protein, they created a mutant library of E. coli tRNA adenosine deaminases (ecTadA), fused it with dCas9...multiplex editing capability with the endogenous tRNA-processing system." Proceedings of the National ...rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID: 30051586. 9. Hua, Kai, et ...

  9. Rett Syndrome

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    ... Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the ...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional... editing, MECP2 reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement...approximately half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR... search bar or sort buttons to find plasmids based on: Expression system (mammalian or bacterial) Insert...human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External Resources Research...

  10. Top Requested Lentivirus and AAV of 2016

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    ...and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate Cas9-expressing cell lines...Cas9-expressing cell line and then transduce this line with lentiviruses expressing various gRNAs. It’...single-vector system). Tips for using this plasmid Cas9 expression is typically cytotoxic, which makes generation...and transgene expression across different cell types (Aschauer et al., 2013). Expression of hM4D(Gi)-mCherry... injected, the DREADD will only be expressed in cells that express cre recombinase. Typically, this feature...to attain cell-specific expression in transgenic animals, where cre expression is restricted to a particular...delivers gRNA, such as lentiGuide-Puro. The alternative single-vector systems typically express Cas9 and...

  11. Save Time with Transient Plant Leaf Transformations

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    Blog Post
    ...observe expression from Agrobacterium, but not the plant. For example, we have seen strong expression of the...transformation Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ...way to overexpress a gene and evaluate its function. In nature Agrobacterium contains a plasmid with a... transgenic cultures or plants, our transient expression method enables us to quickly screen and investigate...reporter gene so that the transformation and gene expression can be tracked. During a transient leaf transformation...localization of your protein of interest. Find all plasmids from the Lee-Parsons lab. Test different leaf...

  12. Antibodies 101: Introduction to Antibodies

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    ...used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies, and...Western blot showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin,...such as parasites or microbes, or to proteins expressed on the surface of cells that have been infected...the protein out of solution, or isolate cells expressing this protein. Read on to learn more about antibodies...context of the immune system, each isotype is expressed at different times of the immune response and ...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...Additionally, the choice of vector can impact the expression level of an antibody (Ayyar et al., 2017). Single-chain...

  13. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post
    ...substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific aminoacyl-tRNA Synthetase ...Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids to Fluorescent Protein Fusions ...However, since tRNA suppression competes with natural chain termination at stop codons, expression levels of...Xenopus oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that...Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have...Fusions Check out Our Fluorescent Protein Guide ...Davis, 2015). By using a novel aminoacyl tRNA synthetase/tRNA pair, a researcher can lure the ribosome...

  14. Viral Vectors 101: Systemic Capsids

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    ...be paired with Cre-expressing transgenic mouse lines to restrict transgene expression to specific tissues...control transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, and...2021) can help restrict expression to your cell type of interest, while miRNA target sites can be used...optogenetic and chemogenetic tools. Dual expression Promoters, miRNAs, enhancers, and switches all take up...determine cell type- or region-specific transgene expression. (a) Several engineered capsids with unique tropisms...specificity. An on/off genetic switch can be used for expression control. (c) Viral delivery methods include focused...efficiency in the brain along with high off-target expression, particularly in the liver, when administered...

  15. Antibody Guide

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    ... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies. Antibodies.... Recombinant expression validation A recombinant expression strategy is used to express the protein of...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic...produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary in... comparison of expression between different samples, by normalizing protein expression to the controls...In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and...

  16. Fluorescent Protein Guide: Empty Backbones

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    ...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...Bacterial Expression iLOV-N1 - Mammalian Expression iLOV-C1 - Mammalian Expression AcGFP1 475 505 28 Prone ... - Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ... - Mammalian Expression Topaz-C1 - Mammalian Expression Topaz-pBAD - Bacterial Expression mTopaz 514 527...

  17. COVID-19 Resources

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    Collection
    ...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike protein...cytokines and related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools SHERLOCK...a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid tools that may be helpful...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...containing SARS-CoV-2 sequences. SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS ...

  18. Common Injection Routes in Mice

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    Blog Post
    ...Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids References Prathipati...catheter, tail vein and femoral cut down injections. Journal of Biosciences and Medicines, 08(09), 131–141. ...

  19. Microbiology Resources

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    ... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene...Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic...resources, or read our guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species...Biology Assembly Standards Guide Bacterial Expression Systems : Find plasmids to perform your experiment...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus...Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica...

  20. How to Write a Scientific Review Article

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    Blog Post
    ....org Check out Our Hot Plasmids Articles to See Compiled Info on New Plasmids Review the CRISPR Field...casually mention “Haery et al., showed that MYC expression was increased…” when discussing the review topic...Field with Our CRISPR Guide Pages ...was invited to write on a particular topic. Alternatively, maybe you’re researching a topic for which ...article doesn’t turn into a textbook. Get the journal’s submission rules for review articles Whether submitting... you have these rules (word limit, formatting guidelines, etc.) you have some criteria to shape the document...easily convert the citation style to whatever the journal requires. Using the (Last name, Year) format also...
Showing: 221 - 240 of 276 results