We narrowed to 277 results for: guide rna expression plasmid

...luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ...Resources Promega Plasmid Collection Fluorescent Proteins Guide Fluorescent Biosensor Plasmids Blog: Luminescence...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid...Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression Renilla...

...used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies, and...Western blot showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin,...such as parasites or microbes, or to proteins expressed on the surface of cells that have been infected...the protein out of solution, or isolate cells expressing this protein. Read on to learn more about antibodies...context of the immune system, each isotype is expressed at different times of the immune response and ...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...Additionally, the choice of vector can impact the expression level of an antibody (Ayyar et al., 2017). Single-chain...

...substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific aminoacyl-tRNA Synthetase ...Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids to Fluorescent Protein Fusions ...However, since tRNA suppression competes with natural chain termination at stop codons, expression levels of...Xenopus oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that...Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have...Fusions Check out Our Fluorescent Protein Guide ...Davis, 2015). By using a novel aminoacyl tRNA synthetase/tRNA pair, a researcher can lure the ribosome...

...be paired with Cre-expressing transgenic mouse lines to restrict transgene expression to specific tissues...control transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, and...2021) can help restrict expression to your cell type of interest, while miRNA target sites can be used...optogenetic and chemogenetic tools. Dual expression Promoters, miRNAs, enhancers, and switches all take up...determine cell type- or region-specific transgene expression. (a) Several engineered capsids with unique tropisms...specificity. An on/off genetic switch can be used for expression control. (c) Viral delivery methods include focused...efficiency in the brain along with high off-target expression, particularly in the liver, when administered...

...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...activation plasmids. To learn more about CRISPR activation and other CRISPR topics, read our CRISPR Guide . Mammalian...ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter...C. elegans ID Plasmid Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

...Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR... CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression: pAC2 , ...129 KB Sontheimer gRNA design and cloning All-in-one plasmid containing expression cassette for NmCas9 and...597 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330)... for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription...endogenous proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available for CREB1, ATF1, GABPA, and RAD21... generation; gRNA design; HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180...

...Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids References Prathipati...catheter, tail vein and femoral cut down injections. Journal of Biosciences and Medicines, 08(09), 131–141. ...

...Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection Chemogenetics plasmids encode genetically engineered...Biosensors Fluorescent Proteins Guide Optogenetics Plasmids Optogenetics Guide Blog: Chemogenetics Blog: Chemogenetics...experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids Browse, sort, or search this table ...ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for chemogenetic experiments, including popular DREADDs and PSAMs. Plasmid... Plasmid Collections ...

....org Check out Our Hot Plasmids Articles to See Compiled Info on New Plasmids Review the CRISPR Field...casually mention “Haery et al., showed that MYC expression was increased…” when discussing the review topic...Field with Our CRISPR Guide Pages ...was invited to write on a particular topic. Alternatively, maybe you’re researching a topic for which ...article doesn’t turn into a textbook. Get the journal’s submission rules for review articles Whether submitting... you have these rules (word limit, formatting guidelines, etc.) you have some criteria to shape the document...easily convert the citation style to whatever the journal requires. Using the (Last name, Year) format also...

...plant plasmid resources at Addgene  Choosing an expression system Choosing an appropriate expression vector....org Find plant plasmids Visit our plant resource page Deposit arabidopsis plasmids with Addgene ...., 2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium tumeficans - a...options, but here are a couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway...for visual selection of transformants based on expression of RFP in the seed (Emami, Yee and Dinneny, 2013...culture you are growing definitely contains your plasmid. Grow lots of Agrobacterium: The more agrobacterium...

... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...'s worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous...backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described below...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the...

...protein-coding genes. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search ...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...some of the plasmids that have been used in these efforts. Addgene's parasite CRISPR plasmid collection...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... gondii genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding...CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA...

...viral capsid, but specificity doesn’t end there: expression from rAAVs also depends on your choice of promoter...the axon to the cell body and driving transgene expression (Tervo et al., 2016). This functionality makes...efficiency in multiple tissues, rapid transgene expression, and can cross blood vessel barriers. It is especially...Systemic Capsids Resources on Addgene.org AAV Guide AAV Data Hub AAV Packaged On Request Browse all ...all in-stock AAV preps Browse all AAV plasmids ..., Bailey, C. G., & Rasko, J. E. J. (2025). An alternate receptor for adeno-associated viruses. Cell, S0092... Mouse Lungs Compared to That of AAV2 Vectors. Journal of Virology, 75(14), 6615–6624. https://doi.org...

...other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA...CRISPR plasmids for transcriptional repression of target genes. CRISPR... CRISPR Interfere CRISPR Plasmids: Interfere...

... Plasmid Collections Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that are...Resources Addgene's plasmid repository contains thousands of fluorescent proteins. Use this guide to learn more...proteins and to find plasmids for your next experiment. Browse Fluorescent Protein Plasmids by Category Empty...Additional Resources at Addgene Optogenetics Guide | Optogenetics Plasmids Use light to activate, inhibit, and ...molecular pathways or cells. Chemogenetics Guide | Chemogenetics Plasmids Use small molecules to activate or .... Luciferase Plasmid Collection Use bioluminescent reporters for studying gene expression, signaling pathways...

...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built... For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting...Maria-Elena Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors...pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...

... Geiss Plasmids SARS-CoV-2 Plasmids Browse the tables below to find popular plasmids expressing genes ... Plasmid Collections COVID-19 – Plasmids and Resources COVID-19 and Coronavirus Plasmids & Resources...Antibodies SARS-CoV-2 Plasmids Other Coronavirus Plasmids Mammalian Targets General Plasmid Tools Pooled Libraries...cytokines and related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools Browse ...versions designed for your expression system. Some of the following plasmids are available to industry,...for-profit entity, please see our Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional... This page includes a table of all spike expression plasmids in our collection that are suitable for generating...

...their seminal work on micro RNAs in C. elegans. The C. elegans work on small RNAs informed and drove work ...modular toolkits build single plasmid targeting vectors that encode the gRNA transcript and the repair template...step as simple as heat-shocking to induce the expression of a Cre recombinase contained within the knock-in... C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to...using modified Cas9 proteins to regulate gene expression (CRISPRi/CRISPRa), visualize specific genomic...Addgene's CRISPR Resources Learn about gRNA design and gRNA optimization for your CRISPR experiment ...depending on perspective – to work with inefficient sgRNAs in my initial direct screening efforts, which were...

...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged... whatever molecule you choose (Figure 1). Just express the fusion protein and add a specific ligand! A...better detect dim structures or low levels of expression with the same imaging workflows you already use...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...Dyeing Through Chemistry Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands GCE4All...10.1038/s41467-023-39655-3  Wilhelm, J., Kühn, S., Tarnawski, M., Gotthard, G., Tünnermann, J., Tänzer, T.,...

...other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system...