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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 328 results for: hal.1

Showing: 221 - 240 of 328 results

  1. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...cleavage (Harrington et al., 2017). Figure 1: Anti-CRISPR proteins (Acr's) can block CRISPR activity... ways to anti-CRISPR proteins in your experiments 1. Reduce off-target effects Prolonged Cas activity ...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ...

  2. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ... present in the sample (APC in the case of Figure 1). In Figure 1A, the population needs to be shifted... decrease the compensation value.   Figure 1: Compensation example with single color control. Flow...Compensating between your fluorescent markers can be challenging! But we hope this guide can help you get started...

  3. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...CRISPR tools.  Distributing and depositing In Figure 1, you can see cumulative numbers of CRISPR plasmid ...new CRISPR depositors each year.    Figure 1: Addgene CRISPR distribution and deposits. (A) Cumulative... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

  4. Of Myc and Men

    Type
    Blog Post
    ...more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising monoclonal antibody...in these species, they found that the N-terminal half of the antigen differs significantly between human...

  5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...crevices proteins on the cell surface. Figure 1: Comparison of common antibody, HCab, and nanobody...Humana Press, pp 133–148. https://doi.org/10.1007/978-1-62703-293-3_10 Walter J et al. (2020) Synthetic nanobodies...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...

  6. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...fluorophores are constantly being engineered. Table 1 lists a few common fluorescent proteins and their ...definitions found in Table 2 may not apply. Table 1: The specific mutations comprising common fluorophores...and when they come into close proximity, the two halves of EGFP undergo folding, maturation, and fluorescence...

  7. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...cycle of one step. Challenges in the LEGO science video process One major challenge when we started was...will bring people together and make these daily challenges a little easier to overcome. We want to encourage...

  8. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...you do just this, probably in less than a minute (1). Green Listed is a new software tool used to design...found through https://wermelinglab.com/.   References 1. Panda, Sudeepta Kumar, et al. "Green listed—a CRISPR...that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...essentiality profiling reveals gene networks and synthetic lethal interactions with oncogenic Ras." Cell 168.5 (2017...

  10. Weighing Reagents Protocol

    Type
    Protocol
    ...resuspended in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may place a microcentrifuge tube on the balance...Pro-Tip If you’re using weighing paper, fold it in half first to create a crease and then place it unfolded...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... necessary for advancing the field. References: 1. Internal initiation of translation of eukaryotic ... of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. Kim et al...segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes...

  12. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... and deep-brain fiber photometry.  Figure 1: Characterization of iGluSnFR4 in mice. A) Scheme ...CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-1 cells (colorectal cancer). Image courtesy of Brianna...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments...

  13. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...be selected for these small ligands. References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of...Biological Applications.” Sensors (Basel). 2012; 12(1): 612–631. PubMed PMID: 22368488. PubMed Central PMCID...technology has been studied and used in assays for over half a century; the Addgene blog has an excellent primer...

  14. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis...translation inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is necessary ...translation is known to be important in development, and Halstead et al. used TRICK to examine translation of Drosophila...Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation. An RNA biosensor...

  15. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...do researchers track fluorescently labeled cells? 1. Epifluorescence microscopy This technique uses a ...of the immune system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals... lymph nodes at different times after antigen challenge, scientists were able to track where GFP labelled...et al. 2016, Cavanagh et al. 2008)? Technical challenges of two photon microscopy Intravital imaging is... at steady state or under physio-pathological challenge. However technical specificities have to be taken...

  16. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...impartial. The best science happens when scientists have 1) the courage to make mistakes and 2) the integrity...of what I saw are that we, as a society, need to: 1) use more citations for out data so our sources are...the University of California Berkeley. It’s been half a year since the march for science on April 22, ...present at the lab bench," Liu, a UM undergraduate, challenged us. I worried the march would be a simple, unquestioning... fingers of skepticism to defend an agenda that halts current data collection, destroys existing data,...

  17. Chemogenetics Guide

    Type
    Guide
    ...out our Chemogenetics Plasmid Collection . Figure 1: General overview of a chemogenetics experiment. Early...bidirectional control of neuronal activity. Table 1. Descriptions of DREADDs and their activity in neurons... while PSAMs and LMOs have an effect for only 0.5–1 hours after delivery. Chemogenetic ligand. The chemogenetic...Ikefuama EC, Slaviero AN, Silvagnoli AD, Crespo EL, Schalau R, Gott M, Tree MO, Dunbar GL, Rossignol J, Hochgeschwender...

  18. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...and also by a lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate...vectors encode a gene which, when expressed, is lethal to the cell. Cloning fragments are inserted into...MCS in the center of this gene, disrupting the lethality. This is similar to α-peptide DNA disruption in...positive colonies do have the plasmid containing the lethal gene. An example is the ccdB gene system commonly...auxotrophic cell lines), or genes which rescue a lethal phenotype. Resources on the Addgene Blog Read...
Showing: 221 - 240 of 328 results