...HIV-1 latent T cell lines, as well as a latent microglial line, treatment with CRISPR/Cas9 SAM increased...Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations Both Inhibit HIV...

...AAV5 is generally the best choice for targeting astroglia. It has additionally been reported to have tropism...tropism for lung, liver, vascular endothelial, and smooth muscle cells (Issa et al., 2023). AAV5 capsids...

...of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ..., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann, P., & Wiegert, J....

...promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or tyrosine-hydroxylase...Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, L. I. (2017). AAV-Mediated...

...successful implementation of GRSMs depends on the reliability of the parts used to build them. We recommend...23034349. PubMed Central PMCID: PMC3914141. 5. Brown, William RA, et al. "Serine recombinases as tools for genome...

...successfully used for genetic manipulation in mammalian cells until 2005 [1]. Like other transposons, ...Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.,” Cell Rep...

...development of viral vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic... differentiated cells such as macrophages and microglia. This property makes the lentivirus an attractive...

... genetic inheritance (1866) and just when the Mendelian genetics was being revived by the botanist Hugo...engineering itself is attributed to SciFi writer Jack Williamson who used it in his 1951 novel Dragon’s Island...

...optical and genetic methods to photo-stimulate mammalian neurons and alter synaptic events: a new technology...Diester. "Optogenetic approaches to study the mammalian brain." Current opinion in structural biology ...

...CRISPR/Cas9 editing strategies in C. elegans As brilliantly and irreverently illustrated in the parable of...innovation for our community. Developments in mammalian and yeast cells using modified Cas9 proteins to...

...Director of Student and Postdoctoral Programs at theJanelia Research Campus of the Howard Hughes Medical ...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...

...or universal. This method is quick, efficient, reliable and cost-effective (Sung, 2006). The sensor cell-based...) Sensitive Assay for Mycoplasma Detection in Mammalian Cell Culture. Anal Chem 84:4227–4232 . https:/...

...cell types, especially immune cells and cells of epithelial origin. Suppressing function, creating tolerance...various signals. Over-production of vascular endothelial growth factors (VEGF) in tumors is attributed...

...experiments, experience has shown that some are more reliable and more efficient at tracking cells. Good two-photon...extension into the small bowel lumen in response to epithelial cell TLR engagement." Journal of Experimental...

...basic biology of optogenetics so if you are not familiar with it, check out the video below). That’s not...coordinates at bregma which allows you to quickly and reliably locate your injection site. We recently purchased...

... available in your lab or the one you are most familiar with. And, hey — if something is working for you...high quantity) – Depending on the experiment, mammalian cell transfections can require quite large amounts...

...identified a protein that enhances prime editing in mammalian cells: the small RNA-binding exonuclease protection...Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and...

...Overall, integrating viral vectors efficiently and reliably generate iPSCs, but they have several safety concerns... and allows for replication of the plasmid in mammalian cells and assists in tethering the vector to the...

..., qPCR needs a steady pair of hands to produce reliable and reproducible data. Here are some tips to help... you’ve done the work to produce excellent and reliable qPCR data, you’ll of course want to share it so...

...Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a mammalian cell line...Although we will use the example of CRISPR/Cas9 in mammalian cells, many of these principles apply to using...dCas9-KRAB is more effective than dCas9 alone for mammalian cell lines. Activate (CRISPRa) Increase expression...expression systems and variables for using CRISPR in mammalian cells. Some of the variables include: Species ...Expression System Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive...expression of Cas9 and/or gRNA in a wide variety of mammalian cell lines Useful for difficult to transfect cell...Video Reprint: Protocol for genomic deletions in mammalian cell lines Select Your Target Sequence and Design...