We narrowed to 532 results for: lias

...with hundreds of variants available. Read on to familiarize yourself with the available FP emission colors...optimized for 37°C, and is therefore most suited for mammalian or bacteria studies, whereas GFPS65T is better...

...prevalent or life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV gene therapy started...trials for devastating genetic diseases such as hemophilia, muscular dystrophy, spinal muscular atrophy,...

... Starting material: plant, microbe, blood, or mammalian cells or tissue Product: total nucleic acids While...purify DNA and RNA from plants, microbes, blood or mammalian cells in 30 seconds or less without using a pipette...

...efficiency through automation.       More Resource Avaliable at Addgene Get help with plasmid transformation... molecular biology reference pages! Resources Avaliable on the Addgene Blog Read more Inside Addgene Posts...

...w303). Developed by Venetia Pliatsika and Isidore Rigoutsos. See Pliatsika and Rigoutsos (2014) (Link ...cloning pCRISPR PDF, 109 KB Mendenhall and Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor...Orkin and Bauer Protocol for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) PDF, 330 KB Parrott...

...populations in the brain. The Allen Institute and the Janelia Research Campus in the USA have used AAV1 and AAV2...of epitope tags for IHC is the availability of reliable primary antibodies for their detection, particularly...

... transcription by blocking its initiation. In mammalian cells, robust repression requires targeting dCas9...delivery systems can be found here “CRISPR 101: Mammalian Expression Systems and Delivery Methods”. Validating...

... creating a robust data pipeline that would be reliable and available for our teams to use, one of our..., and executed code in Jupyter notebooks using familiar libraries such as Pandas, Numpy, and Biopython...

...1053 amino acids) displayed cleavage activity in mammalian cells. SaCas9 produced indels at a similar efficiency...In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102...

...variants are capable of cleaving genomic DNA in mammalian cells and zebrafish embryos, and they can be used...has been adapted for targeted RNA cleavage in mammalian cells. The REPAIR (RNA Editing for Programmable...

...optimum of 30 °C and is therefore inefficient in mammalian cells (usually grown at 37 °C). Smart molecular.... “Conditional Site-Specific Recombination in Mammalian Cells Using a Ligand-Dependent Chimeric Cre Recombinase...

...precise, efficient A•T-to-C•G base editing in mammalian cells and embryos. Nature Biotechnology, 42(4)...editors for efficient T-to-G and C-to-G editing in mammalian cells. Nature Biotechnology, 42(10), 1538–1547...

...differentiation of other CNS cell types, including microglia, astrocytes, and oligodendrocytes, provided that... antibodies for many CNS-resident cell types (microglia are a notable exception) necessitates such molecular...

...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...

...lentiviral expression of the human KRAS gene in mammalian cells. STEP 1: Generate an Entry Clone There are...expression level, and experimental purpose. For mammalian lentiviral expression, we could use a vector like...

...FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari...Tsherniak A, Kadoch C (2018) Interrogation of Mammalian Protein Complex Structure, Function, and Membership...

...thioredoxin reductase and glutathione reductase to faciliate proper folding; may increase multimer formation... Julian Taylor-Parker...

...in the medium or flavin containing proteins in mammalian cells. Generally, autofluorescence is less of .... "A genetically encoded fluorescent probe in mammalian cells." Journal of the American Chemical Society...

...Electronic lab notebooks and researcher's needs The familiar paper-based lab notebook is still a standard approach...information. Does your lab need to meet any specific compliance requirements – e.g. 21 CFR part 11, HIPAA? Answering...

...deliver the alkaline phosphatase gene to airway epithelial cells in mice. This vector transduced ~10% of...A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio A (2008) Serotype-dependent...