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We narrowed to 323 results for: virus

Showing: 221 - 240 of 323 results

  3. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...relatively laborious because multiple plasmids/viruses are needed to deliver all the protein and RNA components...Cas9 activators can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV...vivo CRISPRa (Savell et al., 2019). This dual lentivirus system pairs ubiquitous expression of sgRNA(s... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...

  5. Plasmids 101: Protein tags

    Type
    Blog Post
    .... One of the best optimized is the tobacco etch virus (TEV) protease. A TEV protease cleavage site is ...

  6. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... or not you've efficiently generated infectious virus, plasmids expressing both the Cre and Flp recombinases...and generating new viral vectors. Download the Lentivirus Transfer Vector Production Protocol Lentiviral...pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as well as ...

  7. AAV Packaged on Request

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    Collection
    ... Plasmids Additional Resources Adeno-associated virus (AAV) vectors are safe and useful gene delivery ...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...with the activation domain of the herpes simplex virus VP16 protein (Gossen and Bujard, 1992). This work...Ward 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA Helper virus for monosynaptic tracing. To be coinjected with ...

  10. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...professors, stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to clone...Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis...help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols ...

  15. p53 Pathway

    Type
    Collection
    ...to mutagens like radiation or viruses (e.g., HPV, the human papillomavirus). These mutations interfere ...

  17. Evolution of Lab Techniques

    Type
    Blog Post
    ...and Daniel Nathans. "Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus...
Showing: 221 - 240 of 323 results