We narrowed to 144 results for: transfection

...encoding your protein of interest from Addgene, transfected it into your target cells, now what? How can ...

... With the ability to produce neuron specific transfection using Cre-lox, this new method could allow for...

...showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common ...

...generation R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below...inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture cells... plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target...following plasmids can be used to produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma...plasmids can be used to produce nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma... recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells....

... is easier to implement and ensures that all transfected or transduced cells express both components in...

.... But that doesn’t mean you have to clone and transfect multiple promoter-gRNA constructs in order to ...

...Collection Antibody Blog Posts Antibody Guide Transfection for Recombinant Antibodies Immunocytochemistry...recombinant antibody to room temperature (see Transfection for Recombinant Antibody protocol ). Pro-Tip...

...HMCES, either as a fusion construct or by co-transfection. HMCES forms a chemical crosslink with DNA abasic...

...efficiently transfect. However, note that this method is most useful for cell lines that you can transfect at ...CRISPR system into mammalian cells is to directly transfect expression plasmids encoding your Cas protein ...gRNA, which you generally perform by chemical transfection or electroporation. Expression can be transient...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific cell...These methods are more transient than plasmid transfection or viral vectors, reducing the risk of accumulating...method is helpful in cells that are difficult to transfect, transduce, or may not express common promoters...

...transfer, packaging, and helper plasmid) are transfected into cells which then act as in vitro factories...rare possibility that recombination between the transfected plasmids could lead to the production of replication-competent... AAV Vector topics Addgene Protocols General Transfection AAV Production in HEK293T Cells AAV Purification...and E1B19K genes provide a helper function for transfection-based adeno-associated virus vector production...DNA into eukaryotic cells using viral vectors. Transfection The process of artificially introducing foreign...