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Showing: 361 - 390 of 783 results

  1. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...This post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes...F1000Research uses a novel form of peer review that occurs post-publication and is completely open; the reviewer...

  2. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...blog "Choosing a Good Mentor for Scientists". This post also includes a useful list of questions for a candidate...adding new scientists to their labs. Read the Other Posts in this Blog Series: Advice for Choosing a Research... who are dedicated and interested in science. “Postdoc hires are simpler because of their documented history...thrive during time spent as a graduate student or postdoc. Although the chosen research field is important...not be around much and your real boss might be a postdoc – this isn't bad, but you should know that this...

  11. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...the Addgene blog Browse all of our CRISPR blog posts Find more about fluorescent proteins Get a refresher...refresher on CRISPR with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find ...

  13. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...Nucleocapsid. WPRE Woodchuck hepatitis virus post‐transcriptional regulatory element; sequence that...poly A tract just after the R sequence. This post was written and updated by multiple Addgenies. It...

  14. Clear and Accessible Writing

    Type
    Blog Post
    ...information differently in a poster compared to an online abstract or Addgene blog post.  Here, we are presenting...Clear and Accessible Scientific Writing.” In this post, we’d like to share parts of our workshop with you... scientific writing across all platforms, from posters to papers.  We hope this helps, and happy writing...

  15. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...easy to use biological tools are abundant! In this post, we will review tried-and-true protein expression...tag (dTAG). These control systems function at the post-translational level, unlike most of the other mechanisms...

  18. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela ...Plasmid Technologies Read Other Synthetic Biology Posts  Suggest Plasmid Tools for the Repository Resources...with their environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department...

  20. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...This is the fourth post in the Addgene Blog Mentoring for Scientists Series. I have been thinking a lot...with any questions about mentoring. Read the other posts in the series to get more advice on mentoring: ...group In my travels speaking to grad students and postdocs I have started to talk to trainees more about ...and career stage (e.g. all women, all men, all postdocs, all grad students) or mixed for one or both of...

  21. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...CRISPR, Addgene gave to me: a Cas9 nickase 101 blog post.  On the twelfth day of CRISPR, Addgene gave to ...

  23. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...model generation simpler, as described in an earlier post on generating CRISPR mouse models. For simple knockout...M. Quadros for assistance with editing this blog post. References Yang, Hui, et al. "One-step generation...Resources on the Addgene Blog Read Our Previous Post on CRISPR Mouse Models Learn about Homology Directed...

  24. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger, Addgene Advisory...genes involved in a phenotype of interest. This blog post will discuss differences between these approaches...CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources on Addgene.org Brush up on Your CRISPR...

  25. Antibodies 101: Validation

    Type
    Blog Post
    ...results. But what is appropriately validated? This post will go over some of the general considerations ...antibody? Check out our Selecting an Antibody blog post! Learn more about Antibodies and their many applications... Antibodies 101: Introduction to Antibodies blog post!  Resources on Addgene.org Find plasmids encoding...

  26. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...are being up- or downregulated in a system, detect post-translational modifications, quantify protein levels...immunosorbent assay (ELISA) (Aydin et al., 2015). Post-translational modifications In addition to protein...abundance, westerns detect changes to a protein’s post-translational modifications such as phosphorylation...

  27. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...where you can thrive and do your best work. This post is focused on choosing a lab, but almost all of ...blunders, setting target up to fail, shifting goal posts without telling the target Warning signs while ...Learn about careers in science communication Read posts on mentoring for scientists Resources on Addgene.org...around and accessible for advice and input? Will a postdoc or lab manager be your actual mentor? Is there ...attending career events or being involved in a grad or postdoc association? Will you learn how to run a lab if...a way to grow and learn. Graduate students and postdocs in particular must care about this. The training... than one lab when you choose a grad school or postdoc lab. Having a comparison can be very helpful in...

  28. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded.... For a more detailed review, check out our blog post: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems...prior to irradiation with UV light (365nm) and that post-UV exposure it showed cleavage activity similar ... the Addgene Blog Read Other Optogenetics Blog Posts Read Our Primer on Optogenetics by Derek P. Simon...

  29. The 12 Days of CRISPR

    Type
    Blog Post
    ...CRISPR, Addgene gave to me: updated CRISPR101 blog posts!🎵 On the 4th day of CRISPR, Addgene gave to me:...

  30. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Plasmids: Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale,... Keyence BZ-9000 fluorescence microscope 8 hours post-pulse. The distinct differences of red-to-green ...phosphorylation is one of the most abundant forms of post-translational modifications in cells and research...see the detailed plasmid kit page or read our blog post: Quick, Versatile Plant Transgenesis with GreenGate...Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...Addgene's First 10 Years Addgene’s Top 10 Blog Posts from 2014  ... in the presynaptic region of one neuron and postsynaptic region of a different neuron, come into proximity...
Showing: 361 - 390 of 783 results