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We narrowed to 929 results for: KIN

Showing: 601 - 650 of 929 results

  1. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...sequence (Figure 2A). Since you don’t need additional flanking sequences, shRNA plasmids are a bit smaller, with... than miRNA plasmids, which require additional flanking regions comprised of upstream and downstream genomic...between the red boxes. Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. ...

  2. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...cells of interest. In many experiments you’ll be working with a heterogeneous cell population, for example...While flow cytometry can be used for virtually all kinds of experiments and research fields, for any experiment...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

  3. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...false positives observed in the cloning process, making it easier to produce the constructs you need. Expression...to simplify the protein purification process by making it easy for a researcher to quickly generate N-...carefully chosen to perform statistically relevant benchmarking of cell-free expression systems, and tested ...

  4. Evolution of Lab Techniques

    Type
    Blog Post
    ...nucleotides, called dideoxynucleotides (ddNTPs), lacking the 3’ hydroxyl group required for the extension...sequencing reactions and may use Sanger sequencing. Looking to the future Southern blotting, restriction mapping...mouse lines, such as BAC transgenic or traditional knockins/knockouts, a thing of the past. In the sequencing...

  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...2008). It can also cross the blood-brain barrier, making it useful for applications targeting the CNS (Issa... Tabebordbar, M., Lagerborg, K. A., Stanton, A., King, E. M., Ye, S., Tellez, L., Krunnfusz, A., Tavakoli...Bertera, S., Zhang, J., Chen, C., Papworth, G., Watkins, S., Trucco, M., Robbins, P. D., Li, J., & Xiao...

  6. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... however NLuc’s emission peak is at 460 nm thus making it non-optimal for in vivo imaging. The solution...about to create an in vivo imaging reporter by linking two CyOFP1 molecules to NLuc (CyOFP1-NLuc-CyOFP1...the fluorescent donor for a bioluminescent donor, making BRET probes. Here the bioluminescent donor is unaffected...

  7. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...adds a repressible element to this binary system (making this a repressible binary system). The GAL80 repressor...PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection of VP16... Resources on the Addgene Blog Quick guide to working with Drosophila Terminators and PolyA Signals FLEx...

  8. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...RNase III enzyme named Dicer (Figure 1). The plant kingdom has expanded its repertoire of Dicer enzymes and...silencing effect through targeted mRNA degradation, blocking translation, or chromatin modifications of complementary...researchers studying plants and other organisms lacking this innate immunity need not worry and have benefited...

  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... and SciFi.  Scientists around the world began looking for the magic molecule that serves as the code ...insatiable appetite of the moviegoers for dinosaurs wreaking havoc in a theme park.  Released in 1997, GATTACA... at University of California San Diego. She is working in collaboration with Prof. Alexis Komor and together...

  10. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ... a method relies on accurate ligation of nicks lacking gaps or mismatched base pairs (as in the case for....6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure, reaction...: 16464020.  3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ligases: structural and functional...

  11. Important Considerations When Using AAVs

    Type
    Blog Post
    ...in common? The three most important items when looking for a house and choosing an AAV are… location, .... So how do you choose the right serotype? Keep asking questions!  Table 1: AAV Serotypes and Tropism ...choice of AAV will depend upon which section you’re looking at (striatum, hippocampus and cortex) within the...

  12. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...interest and 50 nucleotides of homology to regions flanking the target site).  The best applications for dsDNA...Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald L. Court. An efficient...Read about Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR...

  14. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...the better the detection of the target marker. Looking at Table 1, we can recognize a couple of fluorophores...brightness levels are often not that great. When thinking of terms like "very bright" vs. "dim" you might...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

  15. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...the template strand. Therefore, I suggest that picking a crRNA complementary to the template strand could....For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG PAM to NGC in ...PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift in the...

  16. Using a Light Microscope Protocol

    Type
    Protocol
    ... until the edges are just out of view. Pro-Tip Looking through a microscope’s eyepiece can take some practice...

  21. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    ...’s Day on the Addgene blog.  We’ve been mostly working remotely for the last year to give our lab and ...in the raffle, you will receive an email from us asking for your mailing address and T shirt size. You...

  22. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...of Addgene's winter holiday celebrations! We're kicking it off by sharing all the incredible guest posts...NEB's DADExperts: Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan...

  23. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020...video channels are also underrated and I recommend checking them out, especially since videos on synthetic...

  24. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ...better way to start off our holiday season than by thanking the twelve guest bloggers and subject matter experts...Toj, a summer intern who shared their experience working at Addgene. An Addgene Summer Internship Matteo...

  25. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...crystal structures demonstrating “the structure is strikingly similar to the design model at atomic resolution...That made it a powerful proof of concept that this kind of protein design is possible. Although Top7 was...

  26. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...culture dish and number of dishes used. Here’s to making AAV packaging a little easier! Click here to download...AAV Packaging and Transfer Plasmids Here New to Making AAV? Check out Addgene’s Step-By-Step Protocol ...

  27. Our New Antibody Mascot is....

    Type
    Blog Post
    ... on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral name (and it can’t hurt if...is included in the final four will receive some rockin’ newly designed Addgene swag. Good luck and happy...

  30. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...was a year like no other, with many scientists working remotely instead of from the lab. Nonetheless, ...viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided to #DeckTheLab...

  32. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ..., check out my Recycling and Innovations posts. Making small changes in your daily habits can feel ineffective...wouldn’t leave your oven on all day to save time making dinner at night, so why leave your lab equipment...

  34. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ...Looking to kick your Zoom background game to the next level? Have you been using Zoom’s default backgrounds...download the image background.     If you’re working with AAV or lentivirus in your research, you might...

  35. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...lentiCRISPR v2, you can take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR...around the world. We thank our many depositors for making these reagents available to the research community...

  36. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...Their muscles showed an increase in inflammatory cytokines as a result of those mild stressors as usual, ...use a good run right about now. And, if you are looking to get your hands on PGC-1a for studies in your...

  37. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...Structural details All this, and scientists are still working out important details in their understanding of...basically happened in a year. It’s incredible.” Looking for CRISPR Plasmids? CRISPR-Cas9 Plasmids available...

  38. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...evolved tRNA adenosine deaminase that is fused to a nicking Cas9. This base editor can be used for A•T to G...Valcyrie is a CRISPR-based marker excision method for marking distinct cell clones developed by the Michalis ...

  39. Let There Be LITE Plasmids

    Type
    Blog Post
    ...Konermann said she is already aware of research groups making use of the new optogenetic tools in C. elegans ...neurons, she offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found ...

  40. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ... Environmental Protection Agency, which started making regulations such as preventing manufacturers from...distribute it to industrial and residential markets. Rethinking our approach to lab waste is something we all...

  42. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding...addition to the Halloween contest, we’ve also carved pumpkins (yes, Blugene made an appearance there too) and...

  44. Science as Art

    Type
    Blog Post
    ...for all to enjoy. The largest competition of this kind is Princeton University’s Annual Art in Science ...Time magazine . Other smaller competitions are now taking place and gathering momentum, including in Cambridge...

  45. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...fluorescent dCas9-KRAB and dCas9-VPR for quantifying and tracking cells that express both the dCas9 construct and... by co-delivering TET1 and protein factors for linking methylcytosine oxidation to DNA repair, which increases...

  46. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...pSiM24, can also be used with biolistic methods, making it useful for engineering plants that are not susceptible...available pCAMBIA and pKYLX vectors, respectively, making it ideal for large gene transfer. The M24 promoter...

  49. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...collaboration. This is why she is excited to be working at Addgene, because it brings scientists together...others. This love of interacting with people and talking about science makes her the perfect Addgenie and...
Showing: 601 - 650 of 929 results