We narrowed to 274 results for: AAV

1. Distribution to Industry

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   Collection

   ...Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from ...

2. Plasmids 101: Using Transposons in the Lab

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   Blog Post

   ... advantage over viral vectors (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, ...

3. Quick Guide to All Things Lentivirus

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   Blog Post

   ...Viral Transductions Learn Why You Might Want to Use AAV in Your Research Lentiviral Vector Uses and Overview...

4. CRISPR Plasmids - Prime Edit

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   Collection

   ... RNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum...

5. Fluorescent Protein Guide: Biosensors

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   .... We also offer ready-to-use AAV preparations of select plasmids and AAV Packaged on Request for many ...Luciferase Plasmids Optogenetics Plasmids Viral Service: AAV Biosensors Fluorescent biosensors are proteins designed...Commun. 2018 Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based...2024.12.16.628673. Olivia Masseck Calcium Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent...voltage imaging. Science. 2019 Aug 1. pii: science.aav6416. Eric Schreiter Voltage Green fluorescent voltage...

6. CRISPR History and Development for Genome Engineering

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   ... smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have distinct PAM sites that...also makes it suitable for multiplexing and use in AAV. Scientists have also developed CRISPR editing technologies...

7. CRISPR 101: Multiplex Expression of gRNAs

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   Blog Post

   ...them advantageous for small capacity vectors like AAV. In addition to the mammalian option described below...

8. How to Deposit Your Plasmids with Addgene

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   Blog Post

   ...in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable strain. For...

9. Deep Dive: qPCR

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   Blog Post

   ...resources on the Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

10. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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    Blog Post

    ...respectively, to obtain PEs small enough to deliver with AAVs but still efficient at long and complex edits. The...

11. CRISPR Guide

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    Guide

    ...efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 ...Mammalian CRISPR libraries have also been created in AAV backbones for in vivo experiments and in a retroviral...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in...

12. Deisseroth INTRSECT Collection

    Type

    Collection

    ...targeting strategy that allows adeno-associated virus (AAV)-borne payloads to be expressed in cells based on... Items 55636 pAAV-EF1a-Cre None Yes 55632 pAAV-Ef1a-mCherry-IRES-Cre None Yes 55637 pAAV-EF1a-Flpo None...None Yes 55634 pAAV-EF1a-mCherry-IRES-Flpo None Yes 55638 pAAV-EF1a-vCre None Yes 55635 pAAV-EF1a-sCre None...Items 55641 pAAV-Ef1a-fDIO EYFP Flp Yes 55640 pAAV-Ef1a-dDIO hChR2(H134R)-EYFP Dre No 55639 pAAV-Ef1a-fDIO...55650 pAAV-hSyn Con/Fon EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn...137134 pAAV-Ef1a-Coff/Fon-mCherry Flp AND NOT Cre Yes 137135 pAAV-Ef1a-oScarlet None Yes 137136 pAAV-Ef1a-Con... 137125 pAAV-Ef1a-sRGECO None Yes 137126 pAAV-Ef1a-Con/Fon-sRGECO Cre AND Flp Yes 137127 pAAV-Ef1a-Con...

13. Molecular Biology Reference

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    Guide

    ...particles (most commonly lentiviral, retroviral, AAV, or adenoviral particles) that can infect your target...

14. Plasmids for Stem Cell Research

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    Collection

    ...Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-...

15. CRISPR Pooled gRNA Libraries

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    Collection

    ...113584 (EFS) 113585 (TBG) Knockout Mouse Chen N/A (AAV) 4 286 Mouse Validation (mVAL) CRISPR Library 159391...

16. CRISPR Guide

    Type

    Collection

    ...efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 ...Mammalian CRISPR libraries have also been created in AAV backbones for in vivo experiments and in a retroviral...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in...

17. Genetic Code Expansion

    Type

    Collection

    ...4xPyOtR-ITR-GFP PylRS M. barkeri Azidolysine (AzK) Mammalian, AAV TAG Abhishek Chatterjee 242002 pSEVA621-AzFRS AzFRS...

18. Our New Antibody Mascot is....

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    Blog Post

    ...  Last month, we introduced you to Blugene and Aavery's newest friend, our Addgene antibody mascot, here...on Feb. 24. Stay tuned!    Original Blugene and Aavery would like your attention, please, as they introduce...AntibodyMakesThree on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral name (and it...

19. General Transfection

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    Protocol

    ...of HEK293T cells optimized for viral production (AAVpro or Lenti-X), but any HEK293T cell line should work...

20. Typing CRISPR Systems

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    Blog Post

    ...362(6416), 839–842. https://doi.org/10.1126/science.aav4294 Hu, C., Myers, M. T., Zhou, X., Hou, Z., Lozen... 363(6422), 88–91. https://doi.org/10.1126/science.aav7271 Yoshimi, K., & Mashimo, T. (2022b). Genome ...