We narrowed to 472 results for: FLU;
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TypeCollection...epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used ... to tag endogenous loci with epitope tags or fluorescent markers. Future Directions It’s amazing to see...
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Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post...laser impacts the magnitude of FSC, SSC, and all fluorescent stains. The cytometer's software will allow you... my analysis. For this measurement, I used a fluorescent dye that can only enter dead cells, while living...interested in measuring a population based on a fluorescent stain, you might be wondering which second measurement... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...purify a variety of peptides including antibiotic efflux pumps and ankyrin seven-repeat proteins: Sjuts...Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives...Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31... -
Antibody Validation for Flow Cytometry
TypeBlog Post... cells were labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were mixed...through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely with target...use in western blot, immunoprecipitation, immunofluorescence and flow cytometry. F1000Research, 13, 817... -
Synthetic Biology - Metabolism
TypeCollection...Include: Biofuels Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table... -
Optogenetics Plasmid Collection
TypeCollection...Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical...neuronal activity, scientists generally attach a fluorescent protein to the opsin in order to track its expression... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection... below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral ... -
Synthetic Biology - Overview
TypeCollection...Mascher Lab Bacillus BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light...Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate... -
Antibody Production
TypeCollection...and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared to... -
Distribution to Industry
TypeCollection... COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson collection... multigene constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for ... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog Post...activated and can cleave fluorescent probes of dsDNA and RNA in trans. Fluorescent signal indicates the presence... -
Microbes: Look, Listen, and Tell
TypeBlog Post...paper thin and very portable microscope. Less fluorescence admittedly, but still so beautiful! Enabling...agar art have a look at our Microbiology and Fluorescent Protein Resources and get started! I learned... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...Pluripotent Stem (iPS) Cells - 2008 was marked by the influx of plasmids for reprogramming differentiated cells...the past 10 years. Biosensors - Plasmids for fluorescent tagging have long been popular with scientists... -
What's New in CRISPR - December 2019
TypeBlog Post... toolkit for targeted genomic integration of fluorescent tags in neurons. Their toolkit named ORANGE (...Editing) mediates targeted genomic integration of fluorescent tags in neurons. Read the paper in bioRxiv ... -
Editor's Choice, September 2016
TypeBlog Post...Advocates Needed to Navigate a Scientific Enterprise in Flux CRISPR Kinome Libraries Available: Pooled and Individual...Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the... -
What's New in CRISPR - September 2019
TypeBlog Post...Cas9 and a donor vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional...interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector. Therefore,... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post... Michael Davidson Fluorescent Protein collection, a ‘One-Stop Shop’ for Fluorescent Proteins, and plasmids... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post... reagents from 20 contributors, including 61 fluorescent probes, representing imaging from 47 different...the Addgene blog Antibodies 101: Multiplex Immunofluorescence Negative Can Be Positive: Open AAV Data with... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...of interest in any way. For example, if green fluorescent protein (GFP) was the reporter gene and green...construct can be something phenotypic (such as a fluorescent protein or something species-specific) or a selectable... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post... optogenetic tool (CsChrimson) attached to a fluorescent indicator (tdTomato), as the effector. They also...expression in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular...