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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 476 results for: FLU;

Showing: 241 - 260 of 476 results

  1. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell...second expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...

  2. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...laser impacts the magnitude of FSC, SSC, and all fluorescent stains. The cytometer's software will allow you... my analysis. For this measurement, I used a fluorescent dye that can only enter dead cells, while living...interested in measuring a population based on a fluorescent stain, you might be wondering which second measurement...

  3. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...purify a variety of peptides including antibiotic efflux pumps and ankyrin seven-repeat proteins: Sjuts...Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives...Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31...

  4. Retrograde AAV viral preps

    Type
    Collection
    ... pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor... pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine... pAAV-hSyn-GRAB_NE1h Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used ... to tag endogenous loci with epitope tags or fluorescent markers. Future Directions It’s amazing to see...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ... cells were labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were mixed...through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely with target...use in western blot, immunoprecipitation, immunofluorescence and flow cytometry. F1000Research, 13, 817...

  7. Synthetic Biology - Metabolism

    Type
    Collection
    ...Include: Biofuels Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table...

  9. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...paper thin and very portable microscope. Less fluorescence admittedly, but still so beautiful! Enabling...agar art have a look at our Microbiology and Fluorescent Protein Resources and get started!  I learned...

  11. Optogenetics Plasmid Collection

    Type
    Collection
    ...Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical...neuronal activity, scientists generally attach a fluorescent protein to the opsin in order to track its expression...

  12. What's New in CRISPR - December 2019

    Type
    Blog Post
    ... toolkit for targeted genomic integration of fluorescent tags in neurons. Their toolkit named ORANGE (...Editing) mediates targeted genomic integration of fluorescent tags in neurons.  Read the paper in bioRxiv ...

  13. Editor's Choice, September 2016

    Type
    Blog Post
    ...Advocates Needed to Navigate a Scientific Enterprise in Flux CRISPR Kinome Libraries Available: Pooled and Individual...Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the...

  14. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...Cas9 and a donor vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional...interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector. Therefore,...

  17. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...luciferase and ZsGreen. Alejandro Balazs 170674 pBOBI-FLuc Express firefly luciferase. Used in lentivirus-based...related pseudovirus assay. David Nemazee 170575 pCMV-FLuc Express firefly luciferase. Used in MLV-based SARS-CoV...

  18. Synthetic Biology - Overview

    Type
    Collection
    ...Mascher Lab Bacillus BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light...Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate...

  19. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...single-domain antibodies genetically fused to fluorescent proteins and used to visualize target antigens...sdAbs in diagnostic applications. For example, fluorescently and radioactively labeled sdAbs have become ...

  20. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...of interest in any way. For example, if green fluorescent protein (GFP) was the reporter gene and green...construct can be something phenotypic (such as a fluorescent protein or something species-specific) or a selectable...
Showing: 241 - 260 of 476 results