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We narrowed to 507 results for: RAP

Showing: 241 - 260 of 507 results
  1. Engaging with science and society at pgEd

    Type
    Blog Post
    ...these tough issues is essential for building a rapport with these communities, which is in turn crucial...plans, or information briefs for policymakers. The rapidly changing landscape of genetics and our consistent...
  2. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...p24 levels in a viral prep is straightforward and rapid as there are several commercially available p24 ...predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1. PubMed PMID: 15291957. PubMed Central...
  3. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...yellow fluorescent proteins tend to photobleach rapidly, which hinders their use for long-term imaging ...serotypes? AAV6 offers broad tissue tropism for gene therapy and ex vivo cell engineering applications and is...
  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... been removed. The degradation and reversal are rapid compared to other systems because the system modulates...Cleveland DW. Inducible, reversible system for the rapid and complete degradation of proteins in mammalian...
  5. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... virus may acquire mutations to escape these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer...approach can augment viral surveillance by enabling rapid assessment of antigenic drift.    Starr et al Cell...
  6. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...days of structural biology, Byron Rubin, a crystallographer working with Jane Richardson, provided an ...popularity was short-lived as powerful molecular graphics packages were soon developed that allowed researchers...
  7. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...embryos were obtained by using optical projection tomography (OPT)  (12) and later Hans-Ulrich Dodt’s lab ...Durchsichtigmachen von menschlichen und tierischen Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson,...
  8. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...recombinant adeno-associated viral vectors." Human Gene Therapy ja (2019). PubMed PMID: 31140327. PubMed Central...genome titers by droplet digital PCR." Human gene therapy methods 25.2 (2013): 115-125. PubMed PMID: 24328707...
  9. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...   Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b...of RNA editing in research and its potential therapeutic uses We are also curious if REPAIR and RESCUE...
  10. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...References 1. Jones, Douglas H., and Bruce H. Howard. "A rapid method for recombination and site-specific mutagenesis..., Peter, Monica Winkler, and Wilfried Bautsch. "Rapid cloning by homologous recombination in vivo." Nucleic...
  11. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...revolution in genome engineering, with new techniques rapidly being developed. Performing a CRISPR experiment...been acquired, genomic editing can be performed rapidly and, in many cases, with reduced chance for off-target...
  12. Lentivirus Production

    Type
    Protocol
    ...first. Pro-Tip The pH of this solution will drift rapidly upon addition of acid or base. Add only a few drops...
  13. Bioinformatics at Addgene

    Type
    Blog Post
    ...various pieces were connected, as this enabled us to rapidly iterate and optimize our pipeline. After reviewing...plasmid sequences on a 96-well plate basis, but for rapid testing and refinements, we specified a select small...
  14. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...one-size-fits-all approach to gene replacement therapy for most patients. But how does one go from “cut...possibilities at the bench and potentially in therapeutic applications. The potential of CRISPR has yet...
Showing: 241 - 260 of 507 results