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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 497 results for: RAP

Showing: 241 - 260 of 497 results

  1. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...days of structural biology, Byron Rubin, a crystallographer working with Jane Richardson, provided an ...popularity was short-lived as powerful molecular graphics packages were soon developed that allowed researchers...

  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...embryos were obtained by using optical projection tomography (OPT)  (12) and later Hans-Ulrich Dodt’s lab ...Durchsichtigmachen von menschlichen und tierischen Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson,...

  3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...revolution in genome engineering, with new techniques rapidly being developed. Performing a CRISPR experiment...been acquired, genomic editing can be performed rapidly and, in many cases, with reduced chance for off-target...

  4. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...   Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b...of RNA editing in research and its potential therapeutic uses We are also curious if REPAIR and RESCUE...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...recombinant adeno-associated viral vectors." Human Gene Therapy ja (2019). PubMed PMID: 31140327. PubMed Central...genome titers by droplet digital PCR." Human gene therapy methods 25.2 (2013): 115-125. PubMed PMID: 24328707...

  7. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...References 1. Jones, Douglas H., and Bruce H. Howard. "A rapid method for recombination and site-specific mutagenesis..., Peter, Monica Winkler, and Wilfried Bautsch. "Rapid cloning by homologous recombination in vivo." Nucleic...

  9. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...analysis is complete, a new screen appears with a graphical representation of the results and a list of the... on the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth analysis...

  11. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  Ultimately, it is...critical analysis of codon optimization in human therapeutics. Trends in Molecular Medicine 20:604–613  https...

  12. Bioinformatics at Addgene

    Type
    Blog Post
    ...various pieces were connected, as this enabled us to rapidly iterate and optimize our pipeline. After reviewing...plasmid sequences on a 96-well plate basis, but for rapid testing and refinements, we specified a select small...

  13. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...one-size-fits-all approach to gene replacement therapy for most patients. But how does one go from “cut...possibilities at the bench and potentially in therapeutic applications. The potential of CRISPR has yet...

  14. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... developed, though, researchers at HuidaGene Therapeutics guessed that fusing MPG to an ABE would allow...recognize T and/or C substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE...

  17. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...known functions has the potential to be used in therapeutics, as it can increase gene expression at certain..., Garnett MJ (2019) Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens. Nature 568...

  19. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Weaker. Commonly found in pBAD vectors. Good for rapid regulation and low basal expression; however, not...to scientists. Plasmids are oftentimes put to therapeutic uses, and in those cases it's important to identify...

  20. Fluorescence Titering Assay

    Type
    Protocol
    ...associated with freeze-thaw. If using frozen virus, rapidly thaw the lentiviral aliquot on ice prior to use...
Showing: 241 - 260 of 497 results