...jacket, home to a questionable collection of memorabilia, even made an appearance during co-founder and...

...gave to me: a miniature CRISPR-Cas system for mammalian use. On the second day of CRISPR, Addgene gave...

...Yit Heng Chooi Lab at the University of Western Australia.    🤩 our lab in Bayliss Building @SMS_UWA is...

... the international controls prescribed by the Australia Group, for these plasmids for the majority of ...

...strategy yields C to A base edits, whereas in mammalian cells, it produces C to G edits. Adenine transversion...recombinase with improved integration efficiencies in mammalian cells. PASTE ( P rogrammable A ddition via S ite-specific...not suitable for all cell types or experiments. Mammalian CRISPR libraries have also been created in AAV...that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available, such as those...examples of activation systems to target genes in mammalian cells include: SunTag system - co-expression of...unique to bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the Feng Zhang ...video reprint: Protocol for genomic deletions in mammalian cell lines Viral service at Addgene Viral plasmids...

... time by quickly relaying key points about an unfamiliar topic or lab process. Along with our role as ...

... to anything goes. We've shared the ones we're familiar with in Table 1.  Table 1: Antibody data repositories...

...manuscripts, talks, etc…!)  Deep Dives You probably are familiar with our 101 series, which introduces common techniques...

...received, we’ll review for technical and legal compliance, and then send you an email with a link to order...

...next million! @CarolineBME @AllostEricSite @LiannaSwanson etc. Missed you Dave... pic.twitter.com/eJbI0lLiGQ...

...microbiology and subsequently obtained a J.D. from William and Mary Law School. Check out our recent Q&A with...

... Let's Go! By Kendall Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick Will You Be My Mentor...

...introduction to plasmids with Director of Biology Lianna Swanson was very popular, with the students excited...

...streamlines the process and saves you time. Anyone affiliated with the recipient organization can create an...

...employment infrastructure. A Stakeholder Workshop at Janelia Research Campus in 2019 solidified the project ...

... high correlation for protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed...

...mix of high-risk, high-reward and low-risk but reliable projects. Chapter 9: Using stem cells as in-vitro...

... for noninvasive imaging of microorganisms in mammalian hosts." Nature 553.7686 (2018): 86.PubMed PMID...

...says). In their hands, BsaI-HF has produced less reliable results. She also notes that for reasons that ...

...Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher at Sanofi Pharmaceuticals...