We narrowed to 532 results for: lias

...proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, 2013). The number of interactions...identifies proximal and interacting proteins in mammalian cells." J Cell Biol 196.6 (2012): 801-810. PubMed...

...musculus) Let’s start with the most preferred mammalian model organism of many researchers: the mouse ...Mus musculus). Mice have many advantages as a mammalian model organism for scientists as they have a relatively...

...deliver the alkaline phosphatase gene to airway epithelial cells in mice. This vector transduced ~10% of...A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio A (2008) Serotype-dependent...

...Mangement for Scientists. Back when I used to do mammalian cell culture, I would often respond to friends...side note, I’d recommend the book, “Bored and Brilliant” for learning more fantastic reasons to remove...

...aExtinction coefficient. bQuantum yield. cDetermined in mammalian cells. dDetermined as effective NIR fluorescence...Brightness of Near-Infrared Fluorescent Proteins in Mammalian Cells. Cell Chem Biol 24, 758-766 e753 (2017)....

...cell populations using the Confetti mouse For mammalian systems, the Clevers lab developed the Confetti...the Fate of Transplanted K14-Confetti Corneal Epithelia in a Mouse Model of Limbal Stem Cell Deficiency...

...facilitate gene expression in bacteria, yeast, mammalian cells, insects, and even Xenopus oocytes. The ...segments of choice into either an E. coli or a mammalian expression vector in a one-pot reaction. Using...

...HIV-1 latent T cell lines, as well as a latent microglial line, treatment with CRISPR/Cas9 SAM increased...Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations Both Inhibit HIV...

... al. described CRISPR-based genome editing in mammalian cell culture. Five years later, PubMed lists more...permanent genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require a specific... this non-specific cleavage does not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also ...has CRISPR plasmids for the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. ...

...nuclease system to facilitate genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ....Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9... are two sets of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene ...In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich...

...visualization inside living or fixed bacterial and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent... Fluorescent Protein Guide for a selection of mammalian and bacterial FRET-related vectors and standards...Paracoccus denitrificans , Methylobacterium extorquens Julia Vorholt 84693 pMyNT-kan Acetamidase promoter Acetamide... that vary in strength and that can be used to reliably drive expression of a gene of interest in E. coli...

... available for expression in mammalian systems and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

...of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ..., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann, P., & Wiegert, J....

...promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or tyrosine-hydroxylase...Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, L. I. (2017). AAV-Mediated...

...successful implementation of GRSMs depends on the reliability of the parts used to build them. We recommend...23034349. PubMed Central PMCID: PMC3914141. 5. Brown, William RA, et al. "Serine recombinases as tools for genome...

...successfully used for genetic manipulation in mammalian cells until 2005 [1]. Like other transposons, ...Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.,” Cell Rep...

... CRISPR Plasmids: CRISPR Transposases (CASTs) Mammalian Bacteria Resources CRISPR-associated transposases... used in bacteria genome editing, though some mammalian systems have been developed. Figure 1: Overview... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...

...Director of Student and Postdoctoral Programs at theJanelia Research Campus of the Howard Hughes Medical ...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...

...optical and genetic methods to photo-stimulate mammalian neurons and alter synaptic events: a new technology...Diester. "Optogenetic approaches to study the mammalian brain." Current opinion in structural biology ...

...CRISPR/Cas9 editing strategies in C. elegans As brilliantly and irreverently illustrated in the parable of...innovation for our community. Developments in mammalian and yeast cells using modified Cas9 proteins to...