We narrowed to 532 results for: lias

...musculus) Let’s start with the most preferred mammalian model organism of many researchers: the mouse ...Mus musculus). Mice have many advantages as a mammalian model organism for scientists as they have a relatively...

...cell populations using the Confetti mouse For mammalian systems, the Clevers lab developed the Confetti...the Fate of Transplanted K14-Confetti Corneal Epithelia in a Mouse Model of Limbal Stem Cell Deficiency...

...facilitate gene expression in bacteria, yeast, mammalian cells, insects, and even Xenopus oocytes. The ...segments of choice into either an E. coli or a mammalian expression vector in a one-pot reaction. Using...

...HIV-1 latent T cell lines, as well as a latent microglial line, treatment with CRISPR/Cas9 SAM increased...Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations Both Inhibit HIV...

...deliver the alkaline phosphatase gene to airway epithelial cells in mice. This vector transduced ~10% of...A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio A (2008) Serotype-dependent...

...aExtinction coefficient. bQuantum yield. cDetermined in mammalian cells. dDetermined as effective NIR fluorescence...Brightness of Near-Infrared Fluorescent Proteins in Mammalian Cells. Cell Chem Biol 24, 758-766 e753 (2017)....

...please do not hesitate to email me at snappe@janelia.hhmi.org." You can find his original blog post below...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...

...Mangement for Scientists. Back when I used to do mammalian cell culture, I would often respond to friends...side note, I’d recommend the book, “Bored and Brilliant” for learning more fantastic reasons to remove...

... al. described CRISPR-based genome editing in mammalian cell culture. Five years later, PubMed lists more...permanent genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require a specific... this non-specific cleavage does not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also ...has CRISPR plasmids for the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. ...

...nuclease system to facilitate genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ....Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9... are two sets of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene ...In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich...

...visualization inside living or fixed bacterial and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent... Fluorescent Protein Guide for a selection of mammalian and bacterial FRET-related vectors and standards...Paracoccus denitrificans , Methylobacterium extorquens Julia Vorholt 84693 pMyNT-kan Acetamidase promoter Acetamide... that vary in strength and that can be used to reliably drive expression of a gene of interest in E. coli...

... available for expression in mammalian systems and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

...successful implementation of GRSMs depends on the reliability of the parts used to build them. We recommend...23034349. PubMed Central PMCID: PMC3914141. 5. Brown, William RA, et al. "Serine recombinases as tools for genome...

...successfully used for genetic manipulation in mammalian cells until 2005 [1]. Like other transposons, ...Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.,” Cell Rep...

...of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ..., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann, P., & Wiegert, J....

...promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or tyrosine-hydroxylase...Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, L. I. (2017). AAV-Mediated...

... CRISPR Plasmids: CRISPR Transposases (CASTs) Mammalian Bacteria Resources CRISPR-associated transposases... used in bacteria genome editing, though some mammalian systems have been developed. Figure 1: Overview... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...

...Director of Student and Postdoctoral Programs at theJanelia Research Campus of the Howard Hughes Medical ...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...

...optical and genetic methods to photo-stimulate mammalian neurons and alter synaptic events: a new technology...Diester. "Optogenetic approaches to study the mammalian brain." Current opinion in structural biology ...

...development of viral vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic... differentiated cells such as macrophages and microglia. This property makes the lentivirus an attractive...