We narrowed to 279 results for: mel

...Miura et al. (2015) developed the ivTRT method to ameliorate this problem. Briefly, you design your donor ...

...2009). If the fluorescence from your target is extremely bright, then the noise likely will not interfere...

...intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light...

...1740-1743. PubMed PMID: 17882145. 2. Zincarelli, Carmela, et al. "Analysis of AAV serotypes 1–9 mediated...

...Evolving the future of antibodies Antibodies are an extremely common lab reagent, so maintaining high-quality...

...quantitative Ca2+ measurements using improved cameleons." Proceedings of the National Academy of Sciences...

...anti-science activists. However, I must say that I was supremely proud of Nashville that morning. Despite the rain...

...2020. Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research. Curr...

...S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek...

... E. coli, the system was used successfully in Tatumella citrea, another species of Enterobacteriaceae,...

..., M. A., Compton-Toth, B. A., Hufeisen, S. J., Meltzer, H. Y., & Roth, B. L. (2004). The highly efficacious...Presymptomatic targeted circuit manipulation for ameliorating Huntington's disease pathogenesis. iScience,...Carpenter, J. C., Snowball, A., Knauss, S., von Schimmelmann, M., During, M. J., Lignani, G., Schorge, S....

...doi.org/10.1016/j.neuron.2021.03.011 Hanlon, K. S., Meltzer, J. C., Buzhdygan, T., Cheng, M. J., Sena-Esteves...

...A., Waldhauer, M. C., Börner, K., Fakhiri, J., Schmelas, C., Dietz, L., Grimm, D., Correia, B. E., Eils...Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N., Kim, J. Y., Chung, A., ...

..., Yan, Z., Zhang, Y., Chow, B. Y., Surek, B., Melkonian, M., Jayaraman, V., Constantine-Paton, M., Wong...

...may cause certain diseases. Sequencing DNA is extremely important when verifying plasmids to ensure each...

...occurring replication-competent rAAV vectors is extremely reduced by dividing the viral genome between the...

...kD single heavy domain fragments derived from a camelid antibody called a heavy chain antibody (HCAb). ...

... 2019 Estimate of time required: ~3 h Workflow Timeline Plate set-up: 2 h qPCR run: 1.5 h Data analysis...°C 30 sec / read plate/ repeat 39x from step 3 / melt curve Example of plate set-up: 1 2 3 4 5 6 7 8 A...accurately determine differences between samples. Melt curve analysis: a single peak should be seen. The...

...Set annealing temperature 5°C below the primer melting temperature (Tm). Set extension step at 1-2 minutes... 3’ primers should be designed to have similar melting temperatures (Tm). Set the annealing temperature...

... Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma #A9414 Luria Broth Agar (LB...37°C for 2 hours. Run digested DNA on 0.8% low melting point agarose gel until you can distinctly see ...