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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 275 results for: mel

Showing: 241 - 260 of 275 results

  1. A Control for All Seasons

    Type
    Blog Post
    ...2009). If the fluorescence from your target is extremely bright, then the noise likely will not interfere...

  3. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...2020. Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research. Curr...

  7. Molecular Biology Reference

    Type
    Guide
    ...may cause certain diseases. Sequencing DNA is extremely important when verifying plasmids to ensure each...only sequence short pieces of DNA. This is not extremely useful when trying to sequence an entire plasmid...

  10. CRISPR Guide

    Type
    Guide
    ...A., Waldhauer, M. C., Börner, K., Fakhiri, J., Schmelas, C., Dietz, L., Grimm, D., Correia, B. E., Eils...Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N., Kim, J. Y., Chung, A., ...

  13. Antibody Guide

    Type
    Guide
    ...kD single heavy domain fragments derived from a camelid antibody called a heavy chain antibody (HCAb). ...

  14. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... 2019 Estimate of time required: ~3 h Workflow Timeline Plate set-up: 2 h qPCR run: 1.5 h Data analysis...°C 30 sec / read plate/ repeat 39x from step 3 / melt curve Example of plate set-up: 1 2 3 4 5 6 7 8 A...accurately determine differences between samples. Melt curve analysis: a single peak should be seen. The...

  15. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...Set annealing temperature 5°C below the primer melting temperature (Tm). Set extension step at 1-2 minutes... 3’ primers should be designed to have similar melting temperatures (Tm). Set the annealing temperature...

  16. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma #A9414 Luria Broth Agar (LB...37°C for 2 hours. Run digested DNA on 0.8% low melting point agarose gel until you can distinctly see ...

  17. Protocol - How to Design Primers

    Type
    Protocol
    ...60% G/C content Start and end with 1-2 G/C pairs Melting temperature (Tm) of 50-60°C Primer pairs should...

  18. Ligation Independent Cloning

    Type
    Protocol
    ...Use web-based primer design software to ensure a melting temperature between 50-60°C for your PCR primers...

  19. Fluorescence Titering Assay

    Type
    Protocol
    ... institution’s biosafety regulations. Workflow Timeline Day 0: Seed 293T cells Day 1: Transduce cells ...

  20. General Transfection

    Type
    Protocol
    ... and different transfection reagents. Workflow Timeline Day 0: Seed HEK293T cells (or a subclone of HEK293T...
Showing: 241 - 260 of 275 results