We narrowed to 279 results for: mel

1. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   ...labeling for neural circuit analysis in Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell...

2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   ...Miura et al. (2015) developed the ivTRT method to ameliorate this problem. Briefly, you design your donor ...

3. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   ...intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light...

4. A Control for All Seasons

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   Blog Post

   ...2009). If the fluorescence from your target is extremely bright, then the noise likely will not interfere...

5. Generating Mouse Models Using CRISPR/Cas9

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   Blog Post

   ...mouse gene-targeting field. Mice have long been extremely useful in the lab – they are relatively small ...

6. Important Considerations When Using AAVs

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   Blog Post

   ...1740-1743. PubMed PMID: 17882145. 2. Zincarelli, Carmela, et al. "Analysis of AAV serotypes 1–9 mediated...

7. Antibodies 101: Antibody Engineering and Directed Evolution

   Type

   Blog Post

   ...Evolving the future of antibodies Antibodies are an extremely common lab reagent, so maintaining high-quality...

8. With an Eye Towards the Future, We Look Back at the March for Science

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   Blog Post

   ...anti-science activists. However, I must say that I was supremely proud of Nashville that morning. Despite the rain...

9. Special Delivery: Fluorophore Targeting for FRET Studies

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   Blog Post

   ...quantitative Ca2+ measurements using improved cameleons." Proceedings of the National Academy of Sciences...

10. CRISPR Guide

    Type

    Guide

    ...A., Waldhauer, M. C., Börner, K., Fakhiri, J., Schmelas, C., Dietz, L., Grimm, D., Correia, B. E., Eils...Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N., Kim, J. Y., Chung, A., ...

11. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    ...S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek...

12. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    ... E. coli, the system was used successfully in Tatumella citrea, another species of Enterobacteriaceae,...

13. Trimmer Lab NeuroMab Collection

    Type

    Collection

    ...2020. Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research. Curr...

14. Molecular Biology Reference

    Type

    Guide

    ...may cause certain diseases. Sequencing DNA is extremely important when verifying plasmids to ensure each...

15. Adeno-associated virus (AAV) Guide

    Type

    Guide

    ...occurring replication-competent rAAV vectors is extremely reduced by dividing the viral genome between the...

16. Antibody Guide

    Type

    Guide

    ...kD single heavy domain fragments derived from a camelid antibody called a heavy chain antibody (HCAb). ...

17. Viral Vectors 101: Systemic Capsids

    Type

    Blog Post

    ...doi.org/10.1016/j.neuron.2021.03.011 Hanlon, K. S., Meltzer, J. C., Buzhdygan, T., Cheng, M. J., Sena-Esteves...

18. AAV Titration by qPCR Using SYBR Green Technology

    Type

    Protocol

    ... 2019 Estimate of time required: ~3 h Workflow Timeline Plate set-up: 2 h qPCR run: 1.5 h Data analysis...°C 30 sec / read plate/ repeat 39x from step 3 / melt curve Example of plate set-up: 1 2 3 4 5 6 7 8 A...accurately determine differences between samples. Melt curve analysis: a single peak should be seen. The...

19. What is Polymerase Chain Reaction (PCR)

    Type

    Protocol

    ...Set annealing temperature 5°C below the primer melting temperature (Tm). Set extension step at 1-2 minutes... 3’ primers should be designed to have similar melting temperatures (Tm). Set the annealing temperature...

20. Protocol - pLKO.1 – TRC Cloning Vector

    Type

    Protocol

    ... Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma #A9414 Luria Broth Agar (LB...37°C for 2 hours. Run digested DNA on 0.8% low melting point agarose gel until you can distinctly see ...