We narrowed to 396 results for: promoter
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TypeBlog Post...was shown that putting the operon under a strong promoter results in high R-body expression (Schrallhammer...
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CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...binding proteins with Cas9 guided by different promoters. The challenge in building genetic circuits is... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...plasmid with a promoter that will be functional in your host organism. Host Relevant Promoters Representative...interest to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated ...proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable marker. They are frequently...T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker Also see more... expression under the metallothionein promoter pFastBac LIC and pFastBac Dual LIC - Vectors from...Gileadi lab Worm unc-54, variety of worm gene promoters C. elegans vector kit - Collection... -
Plant Plasmids and Resources
TypeCollection...containing promoters that drive general and species-specific gene expression (see the column ‘promoter’ in the...for, such as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI... nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots - Vladimir... for features you are looking for, such as the promoter or fluorescent protein of choice. Visit our Fluorescent...Proteins Guide for more. ID Plasmid Description Promoter PI Return to top Other Resources Addgene Blog ... (Link opens in a new window) - The initiative promotes interdisciplinary exchange, open technologies,... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection...Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable...CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...top Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Drosophila ...to top Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Return to top... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...complex, to temporarily inhibit this process and promote copying of the edited strand to the unedited strand... co-express mismatch repair inhibitor MLH1dn Promote desired resolution of heteroduplex ✓ ... -
Adenovirus Plasmids
TypeCollection...of viruses containing transgene under a chosen promoter Vogelstein 16403 pShuttle-CMV Shuttle For production...production of viruses containing transgene under CMV promoter Vogelstein 16404 pAdTrack Shuttle For production...GFP-trackable viruses containing transgene under a chosen promoter Vogelstein 16405 pAdTrack-CMV Shuttle For production...GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407 pAdEasy 2-GFP beta-gal Shuttle... transgene under Neuron-specific enolase (NSE) promoter Bamburg 50959 ShuttleMCP Shuttle For production...viruses containing transgene under mouse cofilin promoter (MCP) Bamburg 62621 pShuttle-CMV-F2A-T2A-Venus... -
TALEN Plasmids and Kits
TypeCollection...DNA sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into...the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter. Truncations ...directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed for in vitro...galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built...for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence to drive efficient TALEN expression...double-transfected mammalian cells. In addition, a T7 promoter was included for generating TALEN mRNAs through...expression. The vectors listed below have the EF1α promoter for efficient mammalian cell expression, and encode... -
CRISPR Plasmids - Drosophila
TypeCollection...lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants...-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused...edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select...variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed... -
CRISPR Plasmids - Plants
TypeCollection...opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas...-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9...edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression... -
CRISPR Plasmids - Base Edit
TypeCollection...Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable...CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources... -
The Pleiades Promoter Project
TypeCollection...Collections Pleiades Promoter Plasmids Pleiades Promoter Project The Pleiades Promoter Project (Link opens...Positive Control CAG promoter pEMS1293 cre N/A CAG promoter pEMS1164 EGFP/cre N/A CAG promoter pEMS1114 EGFP/...NLS N/A CAG promoter pEMS1157 EGFP/NLS N/A CAG promoter pEMS1277 EGFP/NLS N/A CAG promoter pEMS1294 intron-lacZ...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective expression...mouse brain through these human MiniPromoters. Browse the Pleiades Promoter Plasmids using the table below...intron-lacZ/NLS N/A CAG promoter pEMS1488 intron-lacZ/NLS References Portales-Casamar, E., Swanson, D....Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters MiniPromoter Source Gene Construct Reporter Ple2 ADORA2A... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...several weeks which may also depend on transgene or promoter choice. Behaviors you can study using viral vectors... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...its paralog RC2H2 are RNA binding proteins that promote RNA decay and their upregulation is correlated ... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...under the control of Murine Stem Cell retrovirus promoter for mammalian gene knockdown. Vector provided ... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...nuclear localization, binding to the appropriate promoter sequence and activation of gene expression. In... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post...dopamine is an important neurotransmitter that promotes reward-motivated behavior, if the channelrhodopsins... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression pattern... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog Post...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post... had multiple integration events. In addition, promoter choice can also impact transgene expression. The...