We narrowed to 396 results for: promoter
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TypeBlog Post...-loops described above play distinct roles in promoting cellular processes and functions, but they also...
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Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...released into the environment. Various factors promote natural transformation in different bacteria such... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F, Chiu LY, Cox... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...sequence! In bacteria, Cas13d-mediated cleavage promotes collateral cleavage of other RNAs. As with other... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post... 2008). Obligate dimers (such as dTomato) that promote interactions between the FPs interfere with native...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...science and engineering. “Fix the Institutions” promotes gender equality in careers through structural ... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...visualization and expressed from a heart-specific promoter. Fish were labeled with MG-2I, either by incubating... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...was shown that putting the operon under a strong promoter results in high R-body expression (Schrallhammer... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...binding proteins with Cas9 guided by different promoters. The challenge in building genetic circuits is... -
CRISPR Plasmids - Base Edit
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable ...PI Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions... -
The Pleiades Promoter Project
TypeCollection...Collections Pleiades Promoter Plasmids Pleiades Promoter Project The Pleiades Promoter Project (Link opens...Positive Control CAG promoter pEMS1293 cre N/A CAG promoter pEMS1164 EGFP/cre N/A CAG promoter pEMS1114 EGFP/...NLS N/A CAG promoter pEMS1157 EGFP/NLS N/A CAG promoter pEMS1277 EGFP/NLS N/A CAG promoter pEMS1294 intron-lacZ...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective expression...mouse brain through these human MiniPromoters. Browse the Pleiades Promoter Plasmids using the table below...intron-lacZ/NLS N/A CAG promoter pEMS1488 intron-lacZ/NLS References Portales-Casamar, E., Swanson, D....Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters MiniPromoter Source Gene Construct Reporter Ple2 ADORA2A... -
CRISPR Plasmids - Prime Edit
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...complex, to temporarily inhibit this process and promote copying of the edited strand to the unedited strand... co-express mismatch repair inhibitor MLH1dn Promote desired resolution of heteroduplex ✓ ... -
Recombinases AAV Preps
TypeCollection...information about these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40....PI.Cre.rBGe TBG none 8 Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)...DreO-bGHpA Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)...* Janelia Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV...pAAV-EF1a-iFlpV EF1a none 1, PHPeB Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...specific times or locations using cell-specific promoters or inducible systems, you can precisely control...fragments and placed under the control of different promoters. Expression of both fragments in the same cell... lox-STOP-lox shRNA constructs, Cre expression promotes shRNA expression. Gene Switch: These constructs...plasmids based on your expression system of choice, promoter, or search by inducible system (e.g., search for...Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Explore our full...Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Search our full... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression pattern... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...several weeks which may also depend on transgene or promoter choice. Behaviors you can study using viral vectors... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...nuclear localization, binding to the appropriate promoter sequence and activation of gene expression. In... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog Post...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...its paralog RC2H2 are RNA binding proteins that promote RNA decay and their upregulation is correlated ...