We narrowed to 253 results for: sms

1. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

   Type

   Blog Post

   ...specific enzymes are used which trigger cellular mechanisms that generate luminescence. This can then be ...

2. Finding Your Science Policy Path

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   Blog Post

   ...key in science policy. Luckily, there are many mechanisms in place to ensure your effective networking-...

3. With an Eye Towards the Future, We Look Back at the March for Science

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   Blog Post

   ...look for evidence through education.  My only criticisms of what I saw are that we, as a society, need...

4. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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   Blog Post

   ...dynamic processes are largely absent in adult organisms, where tissues structures are more stable and ...

5. A Needle in a Base-Stack: Cas9 Structural Biology

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   Blog Post

   ...Doudna, J. A. (2017). CRISPR-Cas9 Structures and Mechanisms. Annual Review of Biophysics, 46, 505–529. https...

6. 22 Hot Plasmid Technologies from 2014

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   Blog Post

   ...alternative pathway or induce other compensatory mechanisms. How can protein function be dissected while ...

7. CRISPR Guide

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   Guide

   ...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have... In this section, we will introduce the basic mechanisms of CRISPR and some of the ways the CRISPR components...efficiency, or flexibility of the Cas enzyme. Basic Mechanisms of CRISPR The requirements for a CRISPR knockout...Examples of increased fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions ...family members inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition...different locations, by combining other gene editing mechanisms, including transposons, integrases, and recombinases...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...

8. Adeno-associated virus (AAV) Guide

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   Guide

   ...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...later reconstructed within the cell via different mechanisms. One of these methods uses splice site donor ...

9. Molecular Biology Reference

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   Guide

   ...machinery from a particular organism or group of organisms. Meaning, if a plasmid in intended for use in ...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured...

10. Guide to Using Pooled Libraries

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    Guide

    ...being studied. Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance...

11. Plan Your Experiment

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    Guide

    ...these principles apply to using CRISPR in other organisms. First, consider the genetic manipulation that...

12. Optogenetics Guide

    Type

    Guide

    ...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...

13. Adenovirus Guide

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    Guide

    ...G). Different serotypes exhibit varying tissue tropisms, meaning they preferentially infect certain tissues...