We narrowed to 541 results for: SHI

1. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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   Blog Post

   ...changes in the AraC dimer. In doing so, the dimer shifts from one that represses transcription to one that... plate containing the pattern of the image, and shined blue light over the plate. Areas on the plate that...

2. What Do I Do Now? Academic v. Non-Academic Career Decisions

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   ... like you What is different about academia? Publishing in peer reviewed journals is absolutely necessary... important first step. Developing mentoring relationships, especially with peer mentors, to discuss your...

3. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   ... is: could NgAgo replace CRISPR? Such a drastic shift won’t happen overnight, but there are a few reasons...impaired NgAgo activity, with three mismatches abolishing activity. One-guide faithful NgAgo guide loading...

4. Don’t Wait To Launch Your Health Science Startup

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   ...become something tangible.  When you think of entrepreneurship in the 21st century, who do you think of?  ...internal operations and building external strategic partnerships with universities, companies, hospital institutions...

5. Hot Plasmids: FRET-Based Biosensors

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   ...sensors are always bright, they change color as they shift from "off" to "on" in the presence of calcium. This...resulted in the construction of redCALWY-1, a red-shifted FRET sensor for zinc using variants of mOrange2...

6. Celebrate National Book Month with Addgene

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   ...Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music: how we perceive...roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals, and plants...

7. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   ...yeast, as well as mammalian and human cell lines (Nishimura et al., 2009, Natsume et al., 2016).  The Kanemaki...SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018. PubMed PMID: 30166441...

8. The Power Behind NGS Plasmid Validation: seqWell

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   ..., and how we are using this technique in our partnership with Addgene to sequence large numbers of plasmids...Addgene produces plates of plasmid DNA, they are shipped to seqWell, where they are prepared as plexWell...

9. Viral Vectors 101: Inverted Terminal Repeats

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   ...transgene expression and transduction efficiency (Shitik et al. 2023). Transgene expression can be altered.../doi.org/10.1089/hgtb.2016.133. PMID: 28967288. Shitik, E. M., Shalik, I. K., & Yudkin, D. V. (2023). ...

10. Trading Spaces - Visiting Scientist Edition

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    ...researchers are open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach ...grant? A patent? It is never too early to discuss authorship and rights for anything that could be generated...

11. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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    ...Klebsiella spp. Dr. Devarshi Gajjar, The M. S. University of Baroda, India  Devarshi Gajjar has 10 years...

12. Management for Scientists: Delegating is Key

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    ...dependent on the manager to succeed. More people in leadership roles leads to more ideas, initiative and creative...Professional Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through...

13. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    ...in the production of pseudotyped VSV particles (Fukushi et al., 2008).  Browse the Ginkgo Bioworks COVID...COVID-19 Plasmids and Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped Vesicular...

14. Antibodies 101: Monoclonal Antibodies

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    ...2005) Monoclonal Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications, and Information...Monoclonal Antibodies. Monoclonal Antibody Production. Washington (DC): National Academies Press (US); 1999. 1,...

15. Plasmids 101: Screens vs. Selections

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    ... biological system of interest, the so-called “fishing expedition.” Your first question is: to screen ...library resources or our RNAi resources and go fishing! References and resources References Wang, T.,...

16. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    ... Sample6 has changed focus and names, swapped leadership, and grown to 25 members.  What is Sample6?...money to sustain a startup, he went to work for Flagship Ventures – a local Cambridge biotech venture capital...

17. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    ...are not typically persuaded to fire when light is shined on them, but the expression of light-gated ion ... (ChRs) makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons...

18. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    ...cause changes in plasmid copy number. During the shift from exponential to stationary phase, the number... able to better modulate gene expression during shifts in growth rate as compared to a constitutive promoter...

19. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    ...long distances. When it comes to breast cancer, Hoshinho and colleagues found that after injecting breast...cell 21.3 (2012): 309-322. PubMed PMID: 22439926. Hoshino, Ayuko, et al. "Tumour exosome integrins determine...

20. Transferable Skills Guide: Problem Solving

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    ...systematically and logically is as useful for finishing your thesis as it is for helping your team reach... organization Time management Managing a team Leadership Resources on Addgene.org Check out our careers...