We narrowed to 873 results for: LIC

... original CAPTURE and CAPTURE 2.0 methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased rate ...identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously identified with the original... of chromatin interaction networks  A second application for CAPTURE 2.0 is the construction of spatial...temporal changes in chromatin interactions A third application for CAPTURE 2.0 is detection of temporal changes...

... feeling of conflict many of us associate with negotiating (check out more about conflict resolution)....examples of negotiation have been: A job offer conflicts with a pre-planned vacation I’m looking forward...blog More from the transferable skills guide: Conflict Resolution Time management Managing a team Leadership...

... as a helicase. Following recruitment by Cascade, Cas3 nicks the non-target strand. The helicase domain...After these initial steps, Cas3 can perform its helicase and nuclease functions simultaneously, by continuously...Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system. Gene and Genome...

...setting—especially if job applications require teaching experience or high numbers of publications, which may take...people involved is staggering. The process is complicated. If you’re interested in the drug discovery process... proprietary compounds or information. Where publication is expected, the process is similar to academia...

... millions of people die from cancer-related complications each year. Despite these grim statistics, new...non-cancerous cells, such as those within hair follicles and lining the digestive system, causing discomfort...any of these genes through several different applications such as fluorescent protein fusion studies and...of interest to their laboratory. A single mouse click on a gene of interest will direct the investigator...

...loci seem to be associated with the disease and implicate a wide range of cellular processes. The vast majority...oligodendrocytes from human induced pluripotent stem cells. (Ehrlich et al. Proc Natl Acad Sci USA. 2017) Reprogram ...

... and have been adapted for a wide variety of applications - as mutagenesis tools for genetic studies (... The process also creates a 5-bp target-site duplication (TSD), a hallmark characteristic of the Tn7-family...at one or multiple desired genomic loci. Many applications of these systems have already been demonstrated..., 2020), to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology...

...subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426 plasmid requests ...14%). Spikes in plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests...effects fairly well. However, we observed one complication in the China data – there is a period of 1 month...fundamental scientific discovery and high-impact applications.       Alvin Hsu is a graduate student at Harvard...

...early I’m fortunate to live in a town with a good public school system that has made strong efforts in the...simplistic and I would insult the students. Too complicated and I would quickly lose their interest. I won...opinion, it was more important to communicate the applications of CRISPR over the details of how it worked....examples I used in my presentation had only become public in the previous two weeks, which demonstrated to...

...with an abundance of protocols and examples of applications from across disciplines now available. In this...experiment or evaluating ExM images in a new publication.         Why Expansion Microscopy?  Traditional...Tissue expansion is not compatible with in vivo applications and there’s just not much to be done about that...are typically easier to expand than mouse brain slices, which are easier to expand than, say, a C. elegans...

... and are ready to move on to your downstream application — but wait! Do you know how much plasmid DNA ...sample will have minimal impact on downstream applications, but they can impair your ability to get an ...be sure it is compatible with your downstream applications and that you use the same solution for blanking...A230 ratios in RNA preparations on downstream applications. Qiagen Gene Expression Newsletter. Issue 15...

...describe this technology and some of its potential applications? Fujii: We generated a series of retroviral ...the peer-review system of journals and grant applications, for example. However, at the same time, it'.... So, young researchers need to publish good publications in a short period of time and may need to do...available. Visit Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts...

..., mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine et al developed...finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver larger...Brown MT, Deshmukh H, Kemp ML, Bao G (2015) Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human...

...hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection system The Zhang lab...portability. This method can be used for an array of applications including clinical diagnostics (e.g. pathogen...patient samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR allows for...an array of molecular and clinical diagnostic applications. The company Mammoth Biosciences has been started...

...it down until you have something tangible and applicable to your situation.   Thinking Outside the Box...give people more work. Identify your enemies In Felicia Day’s book Embrace your Weird, Day identifies nine...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...

...-strand breaks like those created by Cas12a. Applications of TnpB One of the major challenges in genome...especially valuable for regulatory approval and public acceptance. Recently, viral vectors have been successfully...viral vectors and for multiplexed gene editing applications. As research into TnpB continues to evolve, ...elaborate protocols are available in our earlier publications (Karmakar et al., 2024; Karmakar et al., 2025...

...have real conversations with other scientists. Click Here to Visit Joanne’s AMA I've heard of Reddit,...Anything” was appealing, but also scary. I do a lot of public speaking where I enjoy communicating face to face...posters (this advice included leaving out the term “implicit bias” which is apparently a troll trigger phrase...recommend taking every opportunity you get to speak publically about your work (you can find my tips for a perfect...

...Institute, Public Domain, National Institutes of Health, Wikipedia user Aimecie~commonswiki, Public Domain...let alone the human cost. Here are just a few publications which have since been retracted due to cell ...profiles are available for all the major cell lines on public databases. Image attributions (from top to bottom...

... of a mystical "Force," this quote is equally applicable to many microscopy experiments with fluorescent...How can one ensure that an FP fusion with a non-cytosolic protein functions properly? We suggest starting... non-optimized parental proteins. Our recent publication illustrated the quantitative increase in fluorescent...and Jack Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus...

...resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells...chicken beta actin promoter, and rabbit beta-globin splice acceptor. TRE General expression mRNA Tetracycline... General expression Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite...

...information on NIR FPs, their properties and applications. At the end, there is a Table that summarizes...their enhanced versions named emiRFPs in your applications.  There is a whole set of spectrally distinct... about the characteristics, performance, and applications of near-infrared calcium indicators in this ...of Medicine. Her expertise is development and applications of fluorescent proteins, genetically encoded...

...cell types, and research applications. Labeling neurons with Brainbow The Lichtman group developed the original... Brainbow 3.1 and 3.2 (described in the same publication) are unique because by default, they are not ...level of these XFP is not strong enough for all applications. To resolve this, similar systems have been ...

...al. 2025 under a CC-BY-NC-ND 4.0 International license. While the GENIE team tested and deposited...popular choice for a variety of synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A....DNA vector that contains everything needed to replicate inside a host cell but with space to insert your...bioRxiv, under a CC-BY-NC-ND 4.0 International license. These plasmids are part of the BRAIN Initiative...

...Yizhar, and Karl Deisseroth. "The development and application of optogenetics." Annual review of neuroscience...SSharma, Suveena, and Jinfang Zhu. "Immunologic applications of conditional gene modification technology ...Thompson. "Overview on research and clinical applications of optogenetics." Current protocols in pharmacology... J. Simon, et al. "Silencing neurons: tools, applications, and experimental constraints." Neuron 95.3 ...

...equipment on a lab bench. The best part? Less complicated math. Bioengineering is a field that has been...methods is that they all follow the same general cyclical process (Figure 2). The differences typically ...extensively characterized and validated, with the application data from IPI and other labs easily viewed in...2021). Directed Evolution: Methodologies and applications. Chemical Reviews, 121(20), 12384–12444. https...

...sample. Therefore, when a large number of optical slices are acquired all fluorophores throughout the sample...manufacturers have similar information References 1. Lichtman JW, Conchello JA. Fluorescence microscopy. Nature...2005). PubMed PMID: 16299476. 2. Conchello JA, Lichtman JW. Optical sectioning microscopy. Nature methods...2005). PubMed PMID: 16299478. 9. Richardson DS, Lichtman JW. Clarifying Tissue Clearing. Cell 162, 246-...

... required for a growing organism to make the complicated transition from a clump of cells to a fully differentiated...track where GFP labelled B cells were in mounted slices of these organs. These GFP labelled cells could...been created to fate map Langerhans cells and follicular dendritic cells within the skin and lymph node...of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes." Nature immunology 9.2...

...up. Applications of photoactivatable fluorescent proteins Some of the most important applications for ...level of background fluorescence. For any PA-FP application, the brighter the fluorescence the better. More...photostable green and red states. Beyond these imaging applications PA-FPs have been developed for use in yeast,...

...cellular impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e...innovation, valuable commodities in any NIH grant application! Using FRET, you may gain a new structural understanding...experiments. Donor Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact...discovery–a review of theoretical aspects and recent applications." Current Chemical Genomics and Translational...

...resource for anyone looking to use antibody-based applications in their research.  Like all our eBooks, the...through the process. If you’re confident in your application and protocols, but can’t quite seem to find an...for Your Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place...