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Showing: 391 - 420 of 783 results

  2. To Each HIS Own

    Type
    Blog Post
    ...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in ...information, sign up to receive alerts for antibody posts from our blog!  Resources and References  Resources...

  3. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme. Fast facts – PAM requirement...cleavage). You can read about Cas7-11 in our blog post on its development and use. Fast facts – PAM requirement...

  4. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...degradation of important cellular RNA molecules with post translational modifications. One of the most common...picked your perfect guide; now check out our blog posts on choosing the best Cas9 enzyme to pair with your...

  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...continue where the guide left off - namely, a blog post devoted to the other affinity reagents.  Affinity... antibody?  That is the question… or is it? This post isn’t meant to convert anyone to downsize their ...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    ... subscribe to our CRISPR blog content.   In this post we will cover: Miniature CRISPR-Cas systems SEND...Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    ...researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter. In...!” — Julien Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016,...

  8. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...science career related blog posts Read our science communication blog posts Resources on Addgene.org ...widely. The way you communicate your science at a poster is going to vary depending on the conference you... in an academic lab setting, where student and postdoc turnover happens regularly and it behooves everyone... words like this when starting rotations or my postdoc would have simplified the first weeks or months...you discover during grad school or during your postdoc that you enjoy and have a propensity for cross ...

  9. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...suggests changes in RC2H1 protein expression and post-transcriptional regulation of SM1v1-containing RNAs... by IRE binding proteins, IREB1 and IREB2, which post-transcriptionally regulate iron metabolism pathway...

  10. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...microinjection experiments in S phase zygotes at 18 hr post fertilization. They observed higher targeting efficiency...observed that 41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed a repair...

  11. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... an in-depth review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review ...the other a crRNA array plus a GFP tag. Four weeks post-infection, there was strong expression of Cpf1 and...

  12. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can...leverage this system in new, creative ways. Today’s post will focus on one such strategy--the FLEx switch...

  16. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...IgG2a? Will my choice affect my experiment? In this post we will review some of the main factors that affect...using polyclonal primary antibodies.  I hope this post has explained when and why antibody isotypes matter..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

  17. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...Half (113) expressed in retinal cells four weeks post-injection and 14% had targeted expression in specific...technologies Browse all of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find...mouse retinas were injected into macaques and postmortem human retinas. About 25% of these AAV synthetic...Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez,...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students use molecular models...

  19. 6 Tips for Grant Writing

    Type
    Blog Post
    ...This post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No ...was a PhD student at University College Dublin & a post-doc at Cambridge University where he studied mechanisms...published in a paper, but positive outcomes can include poster presentations, talks about your work, or even the...

  20. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...This post was contributed by guest bloggers Keith Pardee and Alexander A. Green. Zika background First... the Addgene Blog Read other Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab... switches for pathogen detection In 2014, as postdoctoral fellows with Jim Collins and Peng Yin at the...2016). We quickly assembled a team of students, postdocs and professors to move the project forward as ...

  21. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...Taylor-Parker contributed to the writing of this post. Additional Resources on the Addgene Blog Visit...

  24. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...This post was updated on Aug 20, 2020. What genes are important in your phenotype of interest? Many scientists...”, it is certainly the most robust. In this blog post, we will discuss how CRISPR libraries are being ...
Showing: 391 - 420 of 783 results