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We narrowed to 929 results for: Comp;

Showing: 651 - 700 of 929 results

  1. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    ... this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly generating zebrafish transgenic.... Listen to Our Segement on the Tol 2 Gateway-Compatible Toolbox  Hot Plasmid 2: AAV Vectors for tageting...

  2. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...Get Tips on Verifying Your Plasmid Table 1: Comparing Typical Comericial Ladders with the PSU Ladders...1000 Lanes Cost $200-$500  <$10 $20-30 Cost Comparison: While convenient, commercial ladders cost between...

  3. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...experiment. Search engines let you easily find and compare available antibodies from many vendors, while data...Search engine May include validation data  Biocompare Any Any Search engine     Are we missing...

  4. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ... 1. Lab B needs a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave...species for the challenge, but one species always outcompeted the other species.  When he found the chromoprotein...

  5. CRISPR Cheat Sheet

    Type
    Blog Post
    ...genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences of RNA that direct...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases...

  7. Pouring LB Agar Plates

    Type
    Protocol
    ...autoclave. Pro-Tip Once your autoclave cycle is complete, we recommend opening the door to the autoclave...

  8. Western Blot

    Type
    Protocol
    ...proteins >150 kDa. Select Start Run. When the run is complete, select Done. Section 5: Blocking Prepare 1X TBST...

  9. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...decorated office! Below, they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem ...all who participated in the 2018 #DeckTheLab competition. The four winners will receive a holiday care...

  12. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...measure will ever adequately capture a notion as complex as “scientific impact.” As we look ahead to the...Science - The Book Bollen J, et al. "A Principal Component Analysis of 39 Scientific Impact Measures" (2009...

  13. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...’s role in infection also makes it a critical component of vaccines and the target of neutralizing antibodies...Science Translational Medicine have made their complete collection of relevant papers freely available...

  14. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells. The Feng Zhang...efficiency can be controlled by manipulating complementarity between the gRNA and target. Read the paper...

  17. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You have learned...technique and be able to talk and own these accomplishments. Employers and academic departments want to...

  18. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...which decrease the rate of off-target genome edits compared to rat APOBEC1. Now, they’ve engineered SECURE... increased editing efficiency of a target site compared to a mixture of two base editor enzymes in mammalian...

  22. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...plasmids are available either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros...Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications...

  23. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ... online calendar and can be accessed from your computer or smartphone. Evernote allows you to create notes...ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to adapt, likely...

  26. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...Award, the Rare Genomics Institute partners with companies to provide technological resources for those studying...peritoneal mesothelioma. Dr. Nabavi and colleagues have compiled data from primary peritoneal mesothelioma tumor...

  29. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...with the previously established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

  30. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ... next steps and follow up especially in large companies. It is fine to address the cover letter “To Whom...If the organization is small, any person at the company might be able to help you. If the organization ...

  31. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...dire:  The current system is unsustainable, hypercompetition is corroding values, we are shifting away ...such as the National Postdoc Association Core Competencies and the Science Careers’ myIDP). Dr. Galit Lahav...

  33. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    .... “Initial and sustained motivation is a huge component of success.” 2. “Enjoy Teamwork.” Be friendly...friendly, open, sharing, rather than intensely competitive. Be curious and interdisciplinary.   3. “There are...

  36. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...optimal growth temperature of 55 °C. For a brief comparison of GeoCas9 vs. SpCas9, see the table below and...Yes Longevity in Serum?* No Yes Table 1. Comparing SpCas9 and GeoCas9. *For in vitro Cas9 RNP activity...

  37. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...an ‘-omics’ study or is resistant to specific compounds, links to publication references, and cross-references... publications cited. If the information seems incomplete or unclear, Bairoch follow up via email to the...

  39. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...started running on a treadmill. After being with the company for about six months, I learned there was a Genzyme-based...visit us and watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run and play...

  40. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...or leading. All three roles are important to accomplishing any goal or finishing any project. Doers are...Leadership Insights for Engineers, Scientists and Computer Professionals as Leaders, by Ken Graham, Ph.D....

  41. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...training from an organization such as COMPASS. Although COMPASS has a special interest in ocean science...

  42. Advice for Choosing a Research Project

    Type
    Blog Post
    ...typical research project can take several years to complete and has multiple benchmarks. Think about what ...Researchers should  pick a topic that “looks at the complexity of biology and break apart a piece that can address...

  43. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...protospacer adjacent motif (PAM). Once the CRISPR/Cas complex binds to the foreign DNA, a cut is made to destroy...eight evolutionarily conserved CRISPR subtypes comprised of more than 40 cas gene families have been characterized...

  45. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...CellTagging. By constructing lineage trees and comparing them with the gene expression changes of more ...CellTags for tagging two cell populations in a competitive transplant assay in the mouse intestine, demonstrating...

  47. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ...secondary structure and high GC content, but some companies now offer Sanger sequencing conditions which can...

  48. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...given antibody is available through different companies (Bandrowski et al., 2016). It’s also been well...creating an array of educational materials to accompany our antibody collection.  Addgene is sharing our...

  49. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...epitopes available if it is being selected out of a complex mix of proteins that have been extracted from a...Picking the Right Antibody  It can get even more complex if you’re looking for proteins that are interacting...

  50. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome datasets...
Showing: 651 - 700 of 929 results