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We narrowed to 569 results for: CIT

Showing: 261 - 280 of 569 results

  4. Antibody Guide

    Type
    Guide
    ... of 10 Antigen Specificity Antibodies are available in two types of antigen specificity: monoclonal, meaning...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a specific... Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling Visualization...variety in the antibody allowing for their extreme specificity occurs in the B cell itself prior to antibody..., and can be used to increase flexibility or specificity in antibody-based methods. Antibodies can recognize...isotype-specific or even isotype subclass-specific. This specificity can be used to ensure secondary antibodies bind...cost-effective. They are useful when epitope specificity is not a concern or in cases where you may want...

  6. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...for our newsletter and a free plasmid) were very excited about our new viral services. If you’re not familiar...educational content on the blog. Overall, there are many exciting things going on in the world of neuroscience and...

  7. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...starting our viral vector service in 2016...we’re excited to help scientists share their reagents with the... for supporting scientific sharing. We’re very excited for more reagent sharing projects in the years ...

  8. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity and would like to get it working in microglia...development of more and smaller CRISPR tools is an exciting area for future AAV application to epigenetic ...

  10. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades.  So let’s break ...engineered receptors to interact with small molecules to elicit a response in a certain cell type. Chemogenetics...

  11. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...internships share a common set of criteria such as citizenship status, academic year, and grades that serve ...applying to several opportunities. In an age of scarcity, the rational human being will tend to clinch ...

  13. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...perform retrograde tracing, but often at the cost of toxicity, low expression, or poor scalability.  Scientists...neurons within their natural environment, all while reciting their favorite slogan, “look how cool the brain...

  14. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...active throughout the cell cycle and has a higher capacity for repair, as there is no requirement for a repair...RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016...

  15. Adenovirus Guide

    Type
    Guide
    ...due to their well-established methods, relative simplicity of production, and higher titers. However, the... and E4. These modifications aimed to lower immunogenicity issues observed with first-generation rAdV ...vectors and further increase the transgene packaging capacity up to ~10.5 kb. The deleted E2 and E4 regions ...lines. Second-generation rAdV vectors were shown to elicit less immune response when administered in vivo,...vectors are also referred to as "gutless" or high capacity AdV (HCAdV) vectors. Here, all viral sequences...of pAdEasy-2 (Addgene #16401) can increase the capacity of the rAdV vector. pAdEasy-2 does not contain...such as transduction efficiency, tropism and immunogenicity. For example, Ad5/35 and Ad5/26 combine the...

  17. Addgene: The First Twenty Years

    Type
    Blog Post
    ...big plans for our future. And while we’ve been excited to share them with you, we realized our 20th anniversary... millionth item, and had a cumulative 120,000 citations of individual items in our repository, a tribute...

  18. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...protein to create three distinct colors. The specificity of these orthologs is key: since each ortholog...

  19. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...can fail but, in our current setup, there are no explicit instructions on what to do when something fails... We can set ourselves up for success by giving explicit instructions to lab members through the software...
Showing: 261 - 280 of 569 results