Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 526 results for: MEA;

Showing: 261 - 280 of 526 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...signaling pathways to the cell cycle. Good tools for measuring intracellular pH are required in order to determine...aptamer that fluoresces upon binding of a cell-permeable dye. Bunch of Baby Spinach, or BoBS, consists ...reporter plasmid that provides a more sensitive means of studying promoter function, pXPG is an excellent...

  3. Pouring LB Agar Plates

    Type
    Protocol
    ... However, it is also more expensive. Procedure Measure 37g of pre-mixed LB-agar powder per L of molten...agar you’d like to make. The precise mass you measure out will be based on the number of plates you’d...case we spill anything or have small errors in measurement. You should also always make a bit more gel-ager...LB-agar powder. Transfer the LB-agar powder you’ve measured out into an appropriately sized bottle for autoclaving... solution of 100,000 ug/mL (100 mg/mL). Simply measure out 100 mg of ampicillin powder, add it to 1 mL...at a time next to the flame and begin pouring. Measure your desired amount of agar with a pipete for the...

  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... as FRET donors in experiments simultaneously measuring the activities of CaMKII and RhoA during induction... molecular weight markers) that can be used to measure the size of DNA fragments. Depending on which restriction...

  5. Immunocytochemistry

    Type
    Protocol
    ...rocking platform in 500 µL permeabilization buffer. Remove the permeabilization buffer and dispose of it...antibodies raised in a mouse. Reagent Preparation Permeabilization buffer: Dilute 20 µL of Triton X-100 in 10...density before labeling. Section 2: Fixing and permeabilizing cells Gently aspirate the media from the 24...for 5 min in 500 µL PBS on a rocking platform. Permeabilize cells for 10 min at room temperature ( RT ) ...

  6. Pipetting Protocol

    Type
    Protocol
    ...pipette for an accurate measurement in the next step. The second stop is meant to expel excess liquid ...Pipette tips Waste container Containers to hold measured liquid (ex: microfuge tube, bottle, etc.) Labels...tip is so that the same pipette can be used for measuring different samples without cross contamination ...tip into the container you are using to hold the measured liquid (ex: another microcentrifuge tube, a bottle...

  7. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional screens. For more information...several observations: Rapamycin is a membrane-permeable pharmacological agent that can rapidly induction...

  8. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...cells in C57BL/6J mice, but not in BALB/cJ mice, meaning that systemic capsids that rely on LY6A interactions...detectable transduction in the CNS. In the ENS, it has a mean transduction efficiency of ~17%, higher than both...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...gel extraction kit. Elute in 30 μL of ddH 2 O. Measure the DNA concentration. C.4 Ligating and Transforming...TRC-cloning vector from step C.3 (If you were unable to measure the DNA concentration, use 1 μL) 2 μL 10x NEB T4...distribution of any literature by Addgene is not meant to carry with it, and does not grant any license...The distribution of materials by Addgene is not meant to carry with it, and does not grant any license...

  14. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...keep an eye on our blog and social media. In the meantime, thank you all so much for being part of our community...

  17. Call for Guest Bloggers

    Type
    Blog Post
    ...your blog on a variety of social media outlets meaning you’ll have the opportunity to interact with and...

  18. Summer Fun at Addgene!

    Type
    Blog Post
    ...weather is finally warming up in Boston and that means summer is right around the corner. Here at Addgene...

  20. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...up as Addgene's favorite lunch... a BonMe noodle meal, crammed right into the box. Which is your favorite...
Showing: 261 - 280 of 526 results