We narrowed to 527 results for: MEA;

... for self-sponsorship of the I-140 application meaning that the category does not require a foreign national...attractive option.    Your Expert Letters of support can mean that your case gets approved, denied or a Request...

...Biosafety Levels One and Two (BSL-1 and BSL-2) Safety measures for laboratories operating at BSL-1 and BSL-2 ...extract, and precipitate DNA DNA Quantification Measure DNA concentration with a spectrophotometer Restriction...Lentivirus Quantify virus using fluorescence measurements Colony Formation Titering Assay for Lentivirus...by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer of your adeno-associated virus...the Video! AAV Titration by ddPCR Use ddPCR to measure the physical titer of your adeno-associated virus...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...

... However, it is also more expensive. Procedure Measure 37g of pre-mixed LB-agar powder per L of molten...agar you’d like to make. The precise mass you measure out will be based on the number of plates you’d...case we spill anything or have small errors in measurement. You should also always make a bit more gel-ager...LB-agar powder. Transfer the LB-agar powder you’ve measured out into an appropriately sized bottle for autoclaving... solution of 100,000 ug/mL (100 mg/mL). Simply measure out 100 mg of ampicillin powder, add it to 1 mL...at a time next to the flame and begin pouring. Measure your desired amount of agar with a pipete for the...

...signaling pathways to the cell cycle. Good tools for measuring intracellular pH are required in order to determine...aptamer that fluoresces upon binding of a cell-permeable dye. Bunch of Baby Spinach, or BoBS, consists ...reporter plasmid that provides a more sensitive means of studying promoter function, pXPG is an excellent...

...effectively either, but scientists have devised means of co-opting phage genetic systems to facilitate... In contrast, in E. coli, CRISPR is primarily a means of selection that targets cells in which homologous...

...rocking platform in 500 µL permeabilization buffer. Remove the permeabilization buffer and dispose of it...antibodies raised in a mouse. Reagent Preparation Permeabilization buffer: Dilute 20 µL of Triton X-100 in 10...density before labeling. Section 2: Fixing and permeabilizing cells Gently aspirate the media from the 24...for 5 min in 500 µL PBS on a rocking platform. Permeabilize cells for 10 min at room temperature ( RT ) ...

...pipette for an accurate measurement in the next step. The second stop is meant to expel excess liquid ...Pipette tips Waste container Containers to hold measured liquid (ex: microfuge tube, bottle, etc.) Labels...tip is so that the same pipette can be used for measuring different samples without cross contamination ...tip into the container you are using to hold the measured liquid (ex: another microcentrifuge tube, a bottle...

... as FRET donors in experiments simultaneously measuring the activities of CaMKII and RhoA during induction... molecular weight markers) that can be used to measure the size of DNA fragments. Depending on which restriction...

...lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional screens. For more information...several observations: Rapamycin is a membrane-permeable pharmacological agent that can rapidly induction...

...cells in C57BL/6J mice, but not in BALB/cJ mice, meaning that systemic capsids that rely on LY6A interactions...detectable transduction in the CNS. In the ENS, it has a mean transduction efficiency of ~17%, higher than both...

...gel extraction kit. Elute in 30 μL of ddH 2 O. Measure the DNA concentration. C.4 Ligating and Transforming...TRC-cloning vector from step C.3 (If you were unable to measure the DNA concentration, use 1 μL) 2 μL 10x NEB T4...distribution of any literature by Addgene is not meant to carry with it, and does not grant any license...The distribution of materials by Addgene is not meant to carry with it, and does not grant any license...

... courses. The Intro to the Lab Bench series is meant to be an informative introduction for those who ...

...you to help us make the decision between some meaningful names…. Watch the video to find out the final...

...It's December, and that means it's officially the start of Addgene's winter holiday celebrations! We'...

...teams enough space at HQ and for some of us, that means spending more time with our pets. These new “co-...

...keep an eye on our blog and social media. In the meantime, thank you all so much for being part of our community...

...lentiviruses to infect many different types of cells. This means researchers who use VSV-G can deliver their genes...

...scientists. Mammalian cells provide scientists with the means to study biological processes on the cellular level...

...your blog on a variety of social media outlets meaning you’ll have the opportunity to interact with and...

...weather is finally warming up in Boston and that means summer is right around the corner. Here at Addgene...