... methods.   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay...in the saliva (Lucia et al., 2020). Detecting coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​...ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter...2020). An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on...

...reports 5 (2015). BIOFAB plasmid set 3000 Series Confronted with the problem of creating vectors for predictable...Shine-Delgarno sequences used in this so-called bicistronic design (BCD). The 49 vectors in this kit can ...of varying strengths centered within GFP/RFP bicistronic reporters characterized in Cambray et al., 2013...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...

...aggregation in your prep. If your proteins are the wrong size on your gel or blot, Table 1 (at the end of...and keep an eye on it and the leading line (dye front) visible in the sample buffer. You’ll want to run...interest, but you don’t want to run so long your dye front or ladder runs off the gel. Luckily, this is easily...easily monitored in real time, as the dye front (and many ladders!) are visible to the naked eye.   If ... Troubleshooting Suggestion Proteins are the wrong size Proteins may be aggregating Lyse at lower...

...to reduce false positives within a test.   The Bonferroni Correction and the Benjamini-Hochberg procedure...control for large numbers of comparisons. The Bonferroni does this by establishing a significance threshold...However, if many comparisons need to be performed, Bonferroni and Benjamini will greatly reduce family-wise...Dependent Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume 2 (2016). doi.org/10.3389...

... (3): An optics system A fluidics system An electronics system  Cells are first resuspended in a pressurized...fluorophore or fluorochrome-stained protein will emit a stronger photon than a cell expressing low levels of the...triton-x, saponin, or tween. Take care when using strong detergents like triton-x and tween as extended ...FACS In addition to the fluidics, optics, and electronics systems, FACS instruments have specialized components...

...in E. coli by co-expressing the molecule chaperone Skp. Front Cell Infect Microbiol 3: . https://doi.org...Antibodies: Historical Perspective and Future Outlook. Front Immunol 8: . https://doi.org/10.3389/fimmu.2017.01589...biosensor biorecognition elements. Biosensors and Bioelectronics 85:32–45 . https://doi.org/10.1016/j.bios.2016.04.091...

...many scientists operate in a hyper-competitive environment where getting hired hinges on performing near...government scientists to the de-funding of crucial environmental research programs, Canada has been here before...researcher in the University of Michigan Department of Astronomy. She studies how planets form around young stars...community of science supporters is much larger and stronger that I expected. Jennifer Stancill, Ph.D., is ...

...Heinrichs, 2009). When a fluorophore absorbs light, electrons are elevated from a ground state to an excited...After a brief elevation in this excited state, the electron returns to its ground state by emitting a photon...Pyrgiotakis, G., Zhang, Z., Purington, E., Gramlich, W., Grondin, Y., Rogers, R., Bousfield, D., & Demokritou, P...nanocellulose for nanotoxicological studies. Environmental Science: Nano, 6(5), 1516–1526. https://doi....

... Studying cell mobility within these complex environments has never been easy and, for many years, researchers...same laser) cooperate to cause a higher-energy electronic transition in a fluorescent molecule (see figure...adaptive and innate immunity in diverse tissue environments. These studies provide quantitative measurements...-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii." The Journal...

... present in the sample. Other techniques, like electron microscopy and FRET, can offer more information...well described in the closed conformation, recent electron microscopy studies have also found Cas9:RNA complexes...pairing in the seed sequence.   Figure 6: Electron microscopy structure of S. pyogenes Cas9 bound...structure (left, PDB ID 4ZT0) with Cas9:RNA:DNA electron microscopy structure (right, PDB ID 7S4X), viewed...

...particularly energy-intensive systems in a more environmentally responsible manner.  Other ways to make the...in-depth look at Addgene’s current lab-oriented environmental efforts check out Leila Haery’s blog series,... we're always striving to set an example of environmental stewardship.  Additional resources on the Addgene...

... connections between BLA projection neurons and mPFC interneurons can be labelled with an AAV1 Cre virus...AAV9 tdTomato virus in the mPFC.  The AAV9 is so strong that you can even see dendritic spines!  Curious...

...users. Thanks to our guest blogger Cameron MacPherson! Cameron R. MacPherson is Lead Data Scientist...This post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software...worthwhile buying a dedicated machine. On the offline front, there is a Python script called SSFinder, but I...

...Chinmaya Sadangi, a postdoc at the University of Toronto. The Addictive Brain was founded in early 2018 ...Chinmaya Sadangi, a postdoc at the University of Toronto, created The Addictive Brain to spread awareness...Sadangi is currently a postdoc at the University of Toronto and founder of The Addictive Brain. Prior to this...

...ways that I could change the world around me. My strong interest in the intersections of media, art, and...forward but I have learned to push myself to the forefront, no longer allowing myself to just simply exist... an Addgenie. I thought about all that could go wrong with this internship but in the end it turned out...

...Moriarty, an Associate Professor at the University of Toronto, co-founded the COVID-19 Resources Canada site....groups have focused on iterating on methods for coronavirus research, sharing resources for COVID-19 research...our eye recently (not a comprehensive list). #coronavirus #SARSCoV2 #Covid19researchA thread ⬇️⬇️⬇️ — Addgene...

...image the calcium response in the dendrites of T5 neurons by placing the calcium indicator GCaMP6f in the...optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an optogenetic tool...projection-specific brain-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/...

...the workforce, but my main concerns were building strong connections with coworkers and doing something ...to engage with the work of others. Having an environment filled with people who are interested in understanding...interested in knowing you as a person, builds a strong sense of trust between coworkers. Since I trusted...

...Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The first thing you'll...you need to know is your expression system or environment. The host organism will determine the type of...overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression vectors are...

...requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits. ...information resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved...