We narrowed to 1,115 results for: STI

...storage. Biosafety Guide Documentation for your institutional biosafety office and your lab, information on...

...negotiate for a win-win decision An institute/department is investing in you, and is counting on your research... the institute time and energy that would typically be wasted going through some of the questions we mentioned...a junior investigator turned down a faculty position because none of the offering institutes were able...poor outcomes; not enough funds to get started, wasting funds on unnecessary materials, or even a loss ...specific aims. This is actually why a department/institute is interested in you. Your science will define...based on these findings. Most often we suggest estimating the time it will take to secure your first (R-level...greater than $5,000 but this can depend on the institution/funding source. Major equipment includes large...

...we account for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled...mail. Recycle plastic packaging Packaging is a huge contributor to the increase in plastic manufacturing...services don’t offer plastic recycling, specialized companies can recycle plastic-- you just have to find.... This service does cost money, but your institute may still be interested as it offsets the cost of regular...recycling! With the popularity of single-use items, plastic is a substantial portion of lab waste. Despite ...label. To return the box, simply peel your address sticker off the box, remove the ice pack (it’s not recyclable...manufacturing, accounting for 40% of total plastic usage (Geyer et al., 2017). While most single-stream or city ...

...thus individual laser stimulation is essential. In terms of the laser stimulation itself, the output of...battle when using optogentics to answer a research question. The flip side of the optogenetics coin is materials...the dorsal/ventral location of your region of stimulation (the z-axis bregma coordinate). Because the light...it enters tissue, placement above the site of stimulation guarantees that the majority of the opsins will...should be about 0.4-0.5mm above the site of your stimulation. The scatter effect is also an important consideration... polished to significant smoothness to prevent drastic loss of efficiency between patch cable and implant...during the entire protocol, otherwise optical stimulation will be erratic, opsin activation/inactivation...

...Plasmid Collection and Resource Center Do you have suggestions for other plasmids that should be added to this...

...last reviewed: 25 September 2025 Do you have suggestions for other plasmids that should be added to this...

...CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur has deposited a collection of CRISPR arrays...

... host lab or institute provides something that you can’t obtain at your current institute. A common reason...don’t have, or maybe both, a visiting scientist stint may be ideal for you. Here, we will chat about who...scientist, in academia, is someone who visits an institution to perform research there temporarily. The length...The researcher remains employed by their home institute, meaning the host university does not hire or ... pay them (typically). A visiting scientist is distinct from equipment sharing or a collaboration – it...requires re-location of the scientist to the new institute where their typical workdays will be carried out...back to your home lab. Alternatively, the host institute may have a piece of equipment that is custom-built...

...specific statistical test.  Preregistration makes clear the distinction between hypothesis testing work, ... and other questionable research practices (QRPs) can quickly lead us to find statistically significant...encouraged to present that work using hypothesis testing statistical tools that generate a p-value.  As an example...authors are able to make that clear distinction between hypothesis testing and generation, can be sure that...biomedical and psychological sciences were called into question after credible attempts to replicate major findings...instead of the methods. Unreported flexibility and questionable research practices In an attempt to present ...and hypothesis generation, in which data are investigated to look for unexpected trends or differences...

...last reviewed: 25 September 2025 Do you have suggestions for other plasmids that should be added to this...

...colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created...

...process. This early research is still relevant to his work at the Broad Institute today, where he helps researchers... to answer their own research questions. Starting at the Broad Institute For his post-doc, Doench worked...towards the Broad. In order to ask interesting biological questions, you need a tremendous amount of technology...with scientists asking biological questions. Doench loves that he still gets to see data as it’s coming ...the Broad Institute here Read other blog posts about CRISPR Learn more about the Broad Institute and the...the Genetic Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve... School and then applied to work at the Broad Institute: “The model that the Broad was building that had...

...(BRET) were developed by the Johnson Lab . Characteristics of the pHlash reporter protein include: H+...large changes in binding affinity with light stimulation. Indeed oLID showed only a two-fold change in...50 fold change in binding affinity with light stimulation2. The Kuhlman lab created two improved Light ...specific genes via user-defined guide RNAs. Upon stimulation with blue light, the complex recruits a CIB1 ...pUCXKT, removing the stop codons during backbone digestion and providing the missing codons for functional...C-terminal tags. Additionally, by using an adapted destination vector with a heterologous promoter outside of...create an Entry vector containing a promoter for constitutive (CMV, SV40, SFFV) or inducible (CMV-TO) cDNA...

...components into a destination vector in a predefined order. Finally, the destination vectors are used ...the positional groups. Eight final destination vectors, consisting of all four final RVD modules in each... this by selecting the important elements from existing transposon and vector systems and creating an ...transport. pMitoTimer from Yan’s lab, which is constitutively expressed, reports on the balance of biogenesis...fluorescence microscope 8 hours post-pulse. The distinct differences of red-to-green fluorescence in individual...modular system for easier exchange and re-use of existing elements. DNA sequences for a desired component...be used for each Multisite Gateway compatible destination vector for a maximum of 24 elements assembled...

...Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should be added to this...

...interest, and stimulate or manipulate responses through neurological means like electric stimulation, drug administration...Due to the soft nature of the intestines and the requirement for digestive motility to not be disturbed...response to both of these stimulations. The best part? The chemical stimulation was sucrose…..sounds like...validated simultaneously in the brain and the intestines of mice. The result? A very cool tool to advance...Anikeeva lab are microelectronic fibers made from thermoplastic polymers that can work with all the bells and...engineering road map of sorts, giving an idea of the stiffness the fibers would need to be, as well as other ...function (the gut twists and contorts during the digestion of food, while brain tissue is relatively static...

...Hubrecht Institute in The Netherlands used the CRISPR/Cas9 genome editing system to correct the cystic fibrosis...homologous recombination in cultured intestinal stem cells of patients with cystic fibrosis (8).   The difference...therapeutic.” There are many fundamental questions about CRISPR/Cas biology still left to answer as well, she says...Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem ...and precise, researchers are now using it to investigate potential therapeutics for complex genetic diseases...adult stem cells isolated from two patients with cystic fibrosis. They were able to demonstrate functional...stable,” said Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over ...

...sized digestion products (that would co-migrate on a gel) instead of two different sized digestion products...need/want to put the digestion site in the middle of the PCR product, you would still be able to identify...undigested control can help distinguish between a smear and a faint digestion product. Also, depending on...transduced (MOI = 37) and selected with 1 µg/mL blasticidin for 9 days. Single cells from a stable cell pool...then expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed...cells in the population, so some of the cells will still appear green despite having active Cas9 (see tip... target different regions of your gene. I used existing BRAF gRNA viruses. There are a few reasons to ...

...developed this tool, there were so many interesting questions that we could apply [it to].” Using ClonTracer...Library, deposited by Carrie Bhang, a research investigator in the In Vivo Pharmacology group at Novartis...environment” with a strong focus on publishing, but still made it so she could “get exposure to the drug discovery...started using the tool to look at a variety of questions surrounding cancer development, metastasis, and...drug treatments arise during treatment or are preexisting in the parent population prior to the start of...from the same parent cells that represent the preexisting resistant subpopulation 2. Resistant cells among...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

... last reviewed: 12 November 2025 Do you have suggestions for other plasmids that should be added to this...