We narrowed to 958 results for: ache

...love, and even some you’ve likely never heard of. Each entry displays the following information for a Cas... get you started, we’ll give a quick overview of each CasPEDIA feature. Activity features: CasID CasPEDIA... will display both the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently...host factors required for CRISPR processing. For each enzyme, it will also show you how multiplexed arrays...site. In the Experimental Considerations section on each enzyme page, you'll find a link to search Addgene's... as research progresses, but you are invited to reach out to suggest new entries or even new features ...

...non-interacting with) the cells’ own natural translation machinery; these are often incorporated into the cells via...existing GCE tools are not very robust. When using GCE, each component of the experiment system (including media...temperature) may need to be individually optimized for each of the three factors: the ncAA, the tRNA, and the...trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact both translation...scientist interested in, or currently using, GCE to reach out to them and let them know directly what protocols...  Rachel Leeson...

...associations: common colds, COVID-19, norovirus (the stomach flu), and many more. Their infectious nature allows... viral particles, the functional infection unit. Each particle has a viral envelope, which fuses with ...They can also be directly injected into hard-to-reach organs, such as the brain. Secondly, some viruses... post. If a viral vector seems like the right approach for your experiment, you’ll need to decide exactly...adenovirus, and adeno-associated virus (AAV). While each virus allows you to infect cells and deliver cargo...and retro viruses do. They are generally used to achieve transient, high expression of the relevant cargo...

...of ways you could change your variables - your approach, your workflow, or your physical setup - to prevent...protocol when you work and make a checkmark next to each step as you complete it. Yes, this can be incredibly...physical copy. If you’ve got messy handwriting, reach for spreadsheets, printouts, and even label makers...workflow can have a huge impact. Test different approaches, like whether it's better for you to use cold...resistant to. Just like any other experimental approach, use small, incremental changes to understand ... Rachel Leeson...

... disengagement. By tailoring my leadership approach to each person, I was able to create a more productive...differencesManaging a team isn’t “one size fits all”. Each team member has unique motivations, strengths, and...guidance and structure. I quickly learned that my approach needed to adapt based on individual needs. For...tension between two team members with differing approaches—one detail-focused and the other big-picture ...LinkedIn Learning, or edX. Podcasts: HBR IdeaCast Coaching for Leaders More resources on the Addgene blog...

...confirmed the expected features and sequences in each plasmid, none of these constructs have been functionally...those found in the bacterial expression plasmids. Each combination is present in two sets of backbones ...standards are met, we are taking a unique hybrid approach compared to our standard pipeline. For the first... are free of mutation.  Once the samples arrive, each group of plates will have select samples prepared...These new processes have been instrumental in achieving our mission to help science move faster in the...the middle of a global pandemic. And the reach of these plasmids is indeed global. Helping scientists speed...

... I will describe some of the many ways you can approach finding someone to give you advice and guidance...see someone with a great professional presence, approach them for advice on what makes them that way. Is...some of their time. There are many professional coaches who charge for their advice and time. Consider ...highly recommended from someone you trust. A good coach can make a big difference, if you can find one. ...responsible for leading the discussion and for being “coachable”. That means the mentee needs to be open to change...interactions. Watch for the people that are already teaching you and see if there are other ways they can help...

...you can't distinguish the different proteins from each other. To distinguish different target proteins ... analysis, you'll need to use fluorophores that attach to the target. When you design a flow experiment...experiment, you'll need to pick a fluorophore for each target and ensure the fluorophores, together, are able...cells with fluorophore-conjugated antibodies that attach to a specific target. By using a mix of different...respective colors of a few commonly used fluorophores. Each fluorophore has a defined emission maximum. Note...to as bleeding. When two fluorophores bleed into each other, it means that the cytometer could detect ...used in flow cytometry. Let's briefly go through each section of this article again. In the beginning,...

...fluorophores can undergo FRET when they are near each other. How do you label your biomolecule of interest... harder due to tens of thousands of biomolecules each containing multiple binding crevices that can nonspecifically...resulting in site-specific incorporation of the attached fluorophore. However, since tRNA suppression competes...can be enhanced by coupling two NTA molecules to each Cy dye, as well as by extending the length of the...acceptor fluorophore binding sites are adjacent to each other on the same protein and a negative control... 269-272. PubMed PMID: 9657724. 6. Kapanidis, Achillefs N., Yon W. Ebright, and Richard H. Ebright. "Site-specific...Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept...

...optogenetics experiment and what are the tools required for each one? Behavioral Optogenetics can be broken down ...experimentation I’ll discuss some of the key features of each of these steps. Today we’ll discuss selection and...membrane hyperpolarization but unlike HR, this is achieved through hydrogen ion efflux; evidence suggests...expression of your opsin is no longer required to achieve cell-type specific opsin expression. However, the...take as long as 6-8 weeks for sufficient opsin to reach the axon terminal. Again, this must be empirically...neuronal populations using combinatorial viral approaches. Current protocols in neuroscience / editorial...PMC3191888. 9. Zeng H, Madisen L. Mouse transgenic approaches in optogenetics. Progress in brain research. ...

...approximately the length of your average PhD to achieve!) Each field has its own framework: a particular way...audience - like the professor who once decided that to teach high school students PCR, they first had to understand...but the following tips can help:  If there’s a teacher, mentor, or other educator working with your audience...working out, adjust the ask.  I used to do a lot of outreach to student groups and being aware of cognitive... Rachel Leeson...

...existing antibodies. Each cleaved antibody will produce two ~50kD Fab fragments, one for each arm of the antibody...bispecific, meaning that each scFv can target a different antigen; binding of each can be either simultaneous...antibodies can allow you to be flexible in your approach to affinity reagent applications. Whatever affinity...  Rachel Leeson...

...previously developed small molecule degraders that achieve selective degradation of endogenous proteins (notably...Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close proximity with the target protein, ...to co-opt the endogenous ubiquitin proteasome machinery to degrade any protein. We are particularly excited...that are not possible with traditional genetic approaches. In addition, the dTAG system is versatile and...FKBP12F36V-fusion and the ubiquitin proteasome machinery through binding with cereblon, leading to rapid...Confirm the functionality of your fusion chimeras For each protein-of-interest, it is important to determine...

...depending on the conference you’re attending and who approaches you. You might also have cross-team projects ...picture of virtually every container we used, then for each listed: An “official” name for the container to...pretty Addgene specific) The types or purposes of each given container, when applicable Any other relevant...enough. We also had to enforce and maintain it. Teaching people to use words in a new way is not necessarily...Words are important!”  If you try any of these approaches in your lab or workplace, let me know. If you...you have come upon any other effective approaches for clearing up communications lines in your professional...

...be empirically determined for each new batch of 1 mg/mL PEI and for each cell line. Considerations Before...magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter...concentrations and ratios should be optimized for each transfer plasmid. Pro-Tip Endotoxins can inhibit...

...Picogreen quantification on each and every isolated plasmid sample and normalize each to fall within a 10 fold...make use of a pipeline that takes the raw data from each sample in our pool and individually assembles the...plasmid sequences). As with all things at Addgene, each part of this new process involves a significant ...Confirming tags and fusion proteins. We’ll break down each of these steps below. Aligning the sequence The ... making sure to note the appropriate barcode for each sample. About 24 hours later we unload the FASTQ...

...looking for outreach volunteers as well. 4) Blog - Hone your writing skills Take the DIY approach and start...engagement - Participate in science festivals and outreach If you are comfortable talking in public, volunteer...Volunteer – Step into science education Do you aim to reach young people? Try networking with your local education... a blog is not only a great form of scientific outreach, it also helps you to hone your writing skills...Center for Communicating Science. If you choose to teach yourself, check out MIT’s OpenCourseware for course...

...to be less expensive than an ELISA kit. In this approach, viral RNA is first converted to cDNA and then...several different lentiviruses in parallel, this approach should be considered. Similar to the p24 assay... Fluoresence Titering Protocol Another popular approach utilizes the antibiotic resistance genes carried...have integrated into a target cell’s genome. This approach can discriminate multiple integration events, ...underestimating the viral titer. The main drawback of this approach is that it can be quite labor intensive. Target...virus may want to choose more straightforward approaches such as the FACS-based or colony-forming unit...

...-colored protein from the jellyfish Cassiopea xamachana (Blanquet and Phelan, 1987). Eight years later...allows photosynthetically active wavelengths to reach zooxanthellae, the coral’s symbiotic dinoflagellates...Chromoproteins also enhance corals’ resistance to mass bleaching, the loss of their symbiotic algae, during heat...cost and resources are limited or for bioart and teaching applications. Find a chromoprotein for your experiment...agar plate. Image: Liljeruhm et al., 2018. For each chromoprotein, the lab determined the color intensities...protein from the mangrove jellyfish Cassiopea xamachana. Marine Biology 94:423–430 . https://doi.org/10.1007...

...is to make it detectable. Visualization can be achieved using fluorescent microscopy - even in living ...membrane permeabilized in order for the antibodies to reach their intracellular targets. Therefore, it can be...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...make sure that the selected FPs do not influence each other and thus potentially falsify the signal. As...Pages 2. Cooper GM. 2000. The Cell: A Molecular Approach. 2nd Edition. 3.  CureFFI.org. Cell Biology 04... Susanna Bachle...